The measurement of haemoglobin A1c by isoelectric focussing in diabetic patients

Stickland, M. H.; Perkins, C. M.; Wales, J. K.

doi:10.1007/bf00253573

Cited by 29 publications

(4 citation statements)

References 5 publications

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“…We believe that it is especially important to eliminate the labile component before HbA 1c estimation (15)(16)(17)(18)(19)(20), although this idea is not universally accepted (39-41). Some methods, e.g., affinity chromatography, isoelectric focusing, and thiobarbituric acid colorimetry, are not affected by labile HbA, c (11)(12)(13)(14). In the most commonly used method based on charge differences, elimination of labile HbA lc is achieved very simply with our method.…”

Section: Discussionmentioning

confidence: 99%

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Immediate Elimination of Labile HbA1c With Allosteric Effectors of Hemoglobin

Nakashima

Hattori²,

Yamazaki³

et al. 1990

Diabetes

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Our aim was to find a simple method of removing labile glycosylated hemoglobin (HbA1c) from blood samples before it is measured by cation-exchange chromatography. Labile HbA1c is formed by the binding of glucose to the NH2-terminal valine of the beta-chain of HbA. We sought a more competitive binder for the same site to dissociate labile HbA1c to glucose and HbA. Inorganic phosphates were found to have a strong allosteric effect and a great ability to eliminate labile HbA1c. We developed our method with 4 mM tetrapolyphosphate in the hemolyzing solution to eliminate labile HbA1c during the automatic processing (at pH 6 and heated for 2 min at 45 degrees C) of blood samples for HbA1c estimation. This may be useful when estimating HbA1c by the manual method.

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Section: Discussionmentioning

confidence: 99%

“…There are many methods of estimating HbA lc . Although some measure only stable HbA lc and some separate out labile HbA lc , most of these methods are impractical for routine use (11)(12)(13)(14). Cation-exchange chromatography is the most commonly used laboratory method.…”

mentioning

confidence: 99%

Immediate Elimination of Labile HbA1c With Allosteric Effectors of Hemoglobin

Nakashima

Hattori²,

Yamazaki³

et al. 1990

Diabetes

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“…The association of retinopathy and Albustixpositive proteinuria has long been recognized and it has been demonstrated that those Type 1 diabetic patients with an albumin excretion rate above 15 ILg/min are more likely to progress to proliferative retinopathy" as well as to persistent proteinuria. Our findings are consistent with this.…”

Section: Discussionmentioning

confidence: 99%

Glomerular and Tubular Proteinuria in Type 1 (Insulin-Dependent) Diabetic Patients with and without Retinopathy

et al. 1992

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SUMMARY. We compared the urinary excretion of albumin, transferrin, N-acetyl--D-glucosaminidase and o-l-microglcbulin in 78 Type 1 (insulin-dependent) diabetic patients: 39 with retinopathy and 39 without. The two groups were matched for age, sex and duration of diabetes. The patients with retinopathy had increased excretion (median and range) of albumin [1'7 (0' 3-399·1) versus 1·0 (0' 3-116'6) mg/mmol creatinine, P<0'05j, transferrin [114'2 (4'1-37126'2) versus 33·4 (1'0-4176'7) JLg/mmol creatinine, P< 0'01] and N-acetyl-~-D-glucosaminidase [23' 8 (1'1-119'1) versus 15·0 (0'1-65 '1) JLmol/h/mmol creatinine, P

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“…Serum insulin was assayed by a double antibody technique [19] using a human insulin standard. Glycosylated haemoglobin was measured by isoelectric focussing [20].…”

Section: Other Analysesmentioning

confidence: 99%

Decreased insulin sensitivity and muscle enzyme activity in elderly subjects

Kruszynska

Petrányi

Alberti

1988

Eur J Clin Investigation

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Abstract. Skeletal muscle glycogen deposition, and the activation of muscle glycogen synthase and pyruvate deh y drogenase during a hyerinsulinaemic euglycaemic clamp have been measured in six young and six elderly males matched for body mass index, physical activity and diet. Clamp glucose requirement (insulin, 0.1 U kg-' h-') was significantly lower in the older subjects (8.0f0.4 mg kg-' min-I) than in younger subjects (10.5f0.6 mg kg-' min-I, P<0.02). Although the older subjects had a 6.5% decrease in lean body mass, clamp glucose requirement expressed per unit of lean body mass was also significantly decreased in the older subjects (10.2f0.5 vs. 12.4f0.6 mg kg-* min-I, P

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The measurement of haemoglobin A1c by isoelectric focussing in diabetic patients

Cited by 29 publications

References 5 publications

Immediate Elimination of Labile HbA1c With Allosteric Effectors of Hemoglobin

Immediate Elimination of Labile HbA1c With Allosteric Effectors of Hemoglobin

Glomerular and Tubular Proteinuria in Type 1 (Insulin-Dependent) Diabetic Patients with and without Retinopathy

Decreased insulin sensitivity and muscle enzyme activity in elderly subjects

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