The measurement of fibronectin concentrations in human aqueous humor

Abstract

The concentrations of fibronectin in aqueous humor, measured by ELISA which was developed to detect fibronectin, ranged from 5 ng/ml to 100 ng/ml. Aqueous humor was aspirated from human eyes with cataracts and glaucomas using a 26 gauge needle through the peripheral cornea before making the limbal incision into the anterior chamber during surgery. The results of the study show that the average concentration and standard deviation of fibronectin was 0.136 +/- 0.192 microgram/ml in cataract eyes, and 0.962 +/- 0.918 microgram/ml in glaucoma eyes respectively. There was a statistically significant difference between both groups (p = 0.000). However, no significant differences according to age and sex were noted. There was no influence due to preoperative intravenous mannitol injection on fibronectin concentration. The source of aqueous fibronectin is still not clearly known and the mechanism of the higher concentration of fibronectin in glaucoma has not been clearly disclosed, however it is thought that normally present fibronectin is accumulated in the anterior chamber because it can not pass the aqueous outflow pathway, or that fibronectin production may be increased in glaucoma.

Aqueous Humor sCD44 Concentration and Visual Field Loss in Primary Open-angle Glaucoma

“…In POAG aqueous, sCD44, 24 fibronectin, 43 TGF-b, 44,45 plasminogen activator inhibitor-1, 46 TIMP-2, 47 hepatocyte growth factor, 48 and VEGF 49 are increased. Prostaglandin E 2 50 and glutathione 51 levels are decreased.…”

Confirmation of the presence of lens epithelial cells in the anterior chamber after phacoemulsification

“…[19][20][21] Various substances like growth factors, fibronectin, and so on are released into the aqueous humour after the surgery due to the breakdown of blood aqueous humour, which facilitates the proliferation and differentiation of the adhered LECs. [22][23][24] It would be worthwhile to further investigate the role of these live LECs in the incidence and severity of PCO. Our study may have clinical relevance in the context of both ACO and PCO.…”

Role of Fibronectin in Primary Open Angle Glaucoma

“…Increased levels of fibronectin have also been found in the JCT in some patients with POAG and in aqueous humor obtained from patients with glaucoma. Studies comparing the levels of fibronectin in aqueous humor found in patients with cataracts to that of glaucoma patients showed that the levels of fibronectin were nearly sevenfold higher in glaucomatous patients (0.136 ± 0.192 µg/mL vs. 0.962 ± 0.918 µg/mL, respectively) [14,15].…”