1934
DOI: 10.1017/s0007485300012608
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The Measurement and Control of Humidity

Abstract: A few years ago a paper appeared in this Bulletin (Buxton, 1931) describing methods of controlling and measuring humidity. Since that paper was written, we have evolved a number of improvements which tend to make the methods easier in practice, though none of them are novel in principle. It is now known that humidity has diverse and unpredictable effects on many insects. In experimental work it is advisable that humidity should always be measured and controlled even if it is not a factor in the experiment. Mor… Show more

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Cited by 135 publications
(42 citation statements)
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“…alstoniae was studied in the laboratory at temperatures of 20, 25 and 30+ 1 ~ each in combination with a relative humidity (RH) of 50, 70 and 90 + 3%, which was maintained after the method of Buxton and Mellanby (1934). In each experiment, one gravid female was released in a colony ofB.…”
Section: Methodsmentioning
confidence: 99%
“…alstoniae was studied in the laboratory at temperatures of 20, 25 and 30+ 1 ~ each in combination with a relative humidity (RH) of 50, 70 and 90 + 3%, which was maintained after the method of Buxton and Mellanby (1934). In each experiment, one gravid female was released in a colony ofB.…”
Section: Methodsmentioning
confidence: 99%
“…Larvae were moved to fresh diet in new wells each day and given progressively more room to grow by placing them five, three, two and then one larva per well over consecutive days. The tissue-culture plates were kept on racks in a desiccator with a super saturated solution of K 2 SO 4 in its base (to create an environment with 95% relative humidity, Sweetman, 1933) at 35 o C. At the first sign of defaecation (yellow colour in the diet), which occurs just prior to pupation, larvae were gently transferred to clean wells lined with two layers of Kimwipes ® tissue paper and placed in a second desiccator containing a supersaturated solution of NaCl (75% relative humidity, Buxton and Mellanby, 1934) at 35 o C. Prepupae and pupae were checked daily. When close to adult emergence, each pupa was provided with a small quantity of bee bread and sugar candy, so that the newly-emerged adult bee could obtain some food immediately.…”
Section: Larval Bee/avidin Assaymentioning
confidence: 99%
“…They were then observed under different temperature and humidity conditions. The temperatures used were 20, 25, 30 and 35ºC and the relative humidities were maintained at 50, 70, 90% following the procedure of Buxton and Mellanby (1934). All the studies were carried out at 12:12 light: dark photoperiods.…”
Section: Methodsmentioning
confidence: 99%