The MdMYB16/MdMYB1‐miR7125‐MdCCR module regulates the homeostasis between anthocyanin and lignin biosynthesis during light induction in apple

Hu, Yujing; Cheng, Hao; Zhang, Yan; Niu, Shuqing; Wang, Xingsui; Li, Wenjing; Zhang, Jie; Yao, Yuncong

doi:10.1111/nph.17431

Cited by 66 publications

(40 citation statements)

References 66 publications

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“…Both our expression pro les and a previous study on MaMYB54 (TT2L3) and MaMYB39 (MYB4) support this postulation [24]. The homeostasis between anthocyanin and lignin biosynthesis regulated by MYBs was also reported in apples [30].…”

Section: Discussionsupporting

confidence: 88%

Comprehensive Analysis of the MYB Transcription Factor Gene Family in Morus Alba

Liu

Chen

Yidilisi

et al. 2022

Preprint

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The V-myb myeloblastosis viral oncogene homolog (MYB) family of proteins is large, containing functionally diverse transcription factors. In the present study, a genome-wide identification of MYBs in Morus alba was performed. In total 166 MaMYBs were identified, including 103 R2R3-MYBs and four 3R-MaMYBs. Comprehensive analyses, including the phylogenetic analysis with putative functional annotation, motif and structure analysis, gene structure organization, promoter analysis, chromosomal localization, and syntenic relationships of R2R3-MaMYBs and 3R-MaMYBs, provided primary characterization for these MaMYBs. R2R3-MaMYBs covered the subgroups reported for R2R3-MYBs in Arabidopsis and Populus, and had two Morus-specific subgroups, indicating the high retention of MYBs in Morus. Motif analysis revealed high conservative residues at the start and end of each helix and residues consisting of the third helix in R2 and R3 repeats. Thirteen intron/exon patterns (a–m) were summarized, and the intron/exon pattern of two introns with phase numbers of 0 and 2 was the prevalent pattern for R2R3-MaMYBs. Various cis-elements in promoter regions were identified, and were mainly related to light response, development, phytohormone response, and abiotic and biotic stress response and secondary metabolite production. Expression patterns of R2R3-MaMYBs in different organs showed that MaMYBs involved in secondary cell wall components and stress responsiveness were preferentially expressed in roots or stems. R2R3-MaMYBs involved in flavonoid biosynthesis and anthocyanin accumulation were identified and characterized based on functional annotation and correlation of their expression levels with anthocyanin contents. Overall, this work provides an informative reference for further functional dissection of MYBs in Morus.

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Section: Discussionsupporting

confidence: 88%

Comprehensive Analysis of the MYB Transcription Factor Gene Family in Morus Alba

Liu

Chen

Yidilisi

et al. 2022

Preprint

View full text Add to dashboard Cite

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“…As transgenic apple plants exhibit a prolonged juvenile stage, the present work conducted transient transfection for silencing MdMYB306‐like in juvenile ‘Yinv’ fruit and for overexpressing MdMYB306‐like in coloring ‘Yinv’ fruit, mediated by Agrobacterium infection. This approach has been extensively applied to investigate the roles of genes in apple fruit (Hu et al., 2021; Ma et al., 2021; Yang et al., 2021). Compared with the control, the transient silencing of MdMYB306‐like significantly induced anthocyanin accumulation near the injection site; conversely, the overexpression of MdMYB306‐like inhibited anthocyanin accumulation near the injection site (Figure 3a–c), which suggested that MdMYB306‐like plays an inhibitory role in anthocyanin synthesis.…”

Section: Resultsmentioning

confidence: 99%

Apple MdMYB306‐like inhibits anthocyanin synthesis by directly interacting with MdMYB17 and MdbHLH33

Wang

Zhang

et al. 2022

The Plant Journal

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Anthocyanin is one of the most important pigments and nutrients in fruits. Genes encoding R2R3-MYB transcription factors (TFs) are key to anthocyanin regulation. R2R3-MYB activators have been widely studied in the anthocyanin synthesis pathway. However, the mechanism by which R2R3-MYB repressors negatively regulate anthocyanin synthesis remains poorly understood. In the present study, we characterized a subfamily 1 R2R3-MYB anthocyanin repressor gene, MYB306-like, from apple (Malus 3 domestica) and demonstrated its significance in anthocyanin regulation. The MdMYB306-like protein activates the expression of an anthocyanin repressor gene, MdMYB17, and inhibits the expression of MdDFR (an anthocyanin structural gene) by directly binding to the corresponding promoters. The MdMYB306-like protein interacts with MdMYB17 and MdbHLH33 through its N-terminus. In addition, MdMYB306-like interacts with MdbHLH33 and MdMYB17 to enhance its regulatory activities on MdMYB17 and MdDFR, respectively. Our results revealed that the MdMYB306-like protein, in conjunction with MdMYB17 and MdbHLH33, forms a key regulatory module to fine-tune anthocyanin synthesis in apple.

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“…Both our expression profiles and a previous study on MaMYB 54 ( TT2L3 ) and MaMYB39 ( MYB4 ) support this postulation [ 24 ]. The homeostasis between anthocyanin and lignin biosynthesis regulated by cooperation of repressor MdMYB16 and activator MdMYB1 was also reported in apples [ 92 ].…”

Section: Discussionmentioning

confidence: 91%

Comprehensive analysis of the MYB transcription factor gene family in Morus alba

et al. 2022

View full text Add to dashboard Cite

Background The V-myb myeloblastosis viral oncogene homolog (MYB) family of proteins is large, containing functionally diverse transcription factors. However, MYBs in Morus are still poorly annotated and a comprehensive functional analysis of these transcription factors is lacking. Results In the present study, a genome-wide identification of MYBs in Morus alba was performed. In total 166 MaMYBs were identified, including 103 R2R3-MYBs and four 3R-MaMYBs. Comprehensive analyses, including the phylogenetic analysis with putative functional annotation, motif and structure analysis, gene structure organization, promoter analysis, chromosomal localization, and syntenic relationships of R2R3-MaMYBs and 3R-MaMYBs, provided primary characterization for these MaMYBs. R2R3-MaMYBs covered the subgroups reported for R2R3-MYBs in Arabidopsis and Populus, and had two Morus-specific subgroups, indicating the high retention of MYBs in Morus. Motif analysis revealed high conservative residues at the start and end of each helix and residues consisting of the third helix in R2 and R3 repeats. Thirteen intron/exon patterns (a–m) were summarized, and the intron/exon pattern of two introns with phase numbers of 0 and 2 was the prevalent pattern for R2R3-MaMYBs. Various cis-elements in promoter regions were identified, and were mainly related to light response, development, phytohormone response, and abiotic and biotic stress response and secondary metabolite production. Expression patterns of R2R3-MaMYBs in different organs showed that MaMYBs involved in secondary cell wall components and stress responsiveness were preferentially expressed in roots or stems. R2R3-MaMYBs involved in flavonoid biosynthesis and anthocyanin accumulation were identified and characterized based on functional annotation and correlation of their expression levels with anthocyanin contents. Conclusion Based on a comprehensive analysis, this work provided functional annotation for R2R3-MYBs and an informative reference for further functional dissection of MYBs in Morus.

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The MdMYB16/MdMYB1‐miR7125‐MdCCR module regulates the homeostasis between anthocyanin and lignin biosynthesis during light induction in apple

Cited by 66 publications

References 66 publications

Comprehensive Analysis of the MYB Transcription Factor Gene Family in Morus Alba

Comprehensive Analysis of the MYB Transcription Factor Gene Family in Morus Alba

Apple MdMYB306‐like inhibits anthocyanin synthesis by directly interacting with MdMYB17 and MdbHLH33

Comprehensive analysis of the MYB transcription factor gene family in Morus alba

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