The maximum and minimum water content and cell volume of human erythrocytes in vitro

Kageyama, Katsuhiro; Onoyama, Y; Kogawa, Hiroshi; Gotō, Eiji; Tanabe, Kazuyuki

doi:10.1016/0301-4622(89)80044-4

Cited by 21 publications

(14 citation statements)

References 2 publications

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“…Individual cell masses were obtained by multiplying their estimated volumes with their physical density. The density of each cell was taken to be 1 g/cm 3 , the same as distilled water, as water is the major component of eukaryotic cells (Omori & Ikeda 1984, Kageyama et al 1989. Total ciliate biomass per milliliter was calculated as the sum of the masses of all ciliate cells found per milliliter of surveyed.…”

Section: Ciliate Samplingmentioning

confidence: 99%

Substratum‐dependent responses of ciliate assemblages to temperature: a natural experiment in Icelandic streams

et al. 2015

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1. Ciliate assemblages play a significant role in the microbial food web. The effects of environmental temperature on assemblage composition may be influenced by abiotic factors such as seasonality and disturbance, but the effects of temperature on ciliate assemblages found on different substrata have not been explored. Sandy bottoms and submerged rocks harbour dissimilar ciliate assemblages, and it might be expected that their ciliate assemblages will respond differently to temperature. 2. We studied how alpha diversity, beta diversity and total biomass of ciliate protist assemblages found on sandy bottoms and submerged rocks differed in 13 geothermally heated streams in Iceland whose mean temperatures range from 5 to 20°C. We recorded number of operational taxonomic units (OTUs) and measured the size of cells in ciliate assemblages from both substrata. 3. Effects of temperature on natural ciliate assemblages were substratum dependent. On rock surfaces, both total ciliate biomass and alpha diversity declined with increasing temperature, and beta diversity increased with increasing temperature difference due to OTU nestedness (assemblages from warm streams being composed chiefly of subsets of the OTUs found in colder streams). In sandy substrata, however, ciliate assemblage composition was independent of temperature. 4. Substratum-specific responses may be due to differences in mechanical disturbance, nutrient availability or exposure to invertebrate grazers. Rock-surface assemblages may be more exposed to the flow and retain less nutrient than those of sandy substratum; thus, they may be more strongly resource limited and more responsive to direct effects of temperature on metabolism. Alternatively, rocksurface assemblages may be more exposed to grazing by invertebrates, which intensifies with temperature. 5. Our study highlights the need to account for environmental context such as substratum type to fully understand the effect of temperature on microbial assemblages in streams. Future increases in global temperatures may affect fresh waters differently depending on their prevalent substratum. Those dominated by hard substrata may have their ciliate assemblages, and thus, food-web structures and ecosystem functioning more strongly affected by warming relative to systems dominated by soft substrata. 2 ABSTRACT 1. Ciliate assemblages play a significant role in the microbial food web, and the effects of environmental temperature on their composition can be influenced by abiotic factors such as seasonality and disturbance. The effects of temperature on ciliate assemblages found on different substrates, however, have not been explored. Different substrate types such as sandy bottoms and submerged rocks harbour dissimilar ciliate assemblages; thus we could expect ciliate assemblages from these two substrates to respond differently to temperature.2.We studied how alpha diversity, beta diversity, and total biomass of ciliate protist assemblages found on sandy bottoms and submerged rocks changed in 13 geother...

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Section: Ciliate Samplingmentioning

confidence: 99%

Substratum‐dependent responses of ciliate assemblages to temperature: a natural experiment in Icelandic streams

et al. 2015

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“…In vitro experiments have shown that hypoosmotic solutions cause erythrocyte lysis due to the passive movement of free water into the cytoplasm, causing the cell to assume a spheroid shape. In one experiment, it was found that maximal membrane surface tension was exceeded at mean cellular volume of 98.5 fL, leading to cell rupture (7). Other investigations have shown that normal erythrocytes will begin hemolyzing when placed in a saline concentration of Յ0.5%, corresponding to an osmolality threshold of approximately 170 -180 mOsm/L (7,8).…”

Section: Discussionmentioning

confidence: 96%

“…In one experiment, it was found that maximal membrane surface tension was exceeded at mean cellular volume of 98.5 fL, leading to cell rupture (7). Other investigations have shown that normal erythrocytes will begin hemolyzing when placed in a saline concentration of Յ0.5%, corresponding to an osmolality threshold of approximately 170 -180 mOsm/L (7,8). The osmotic hemolysis that occurred in the cases described, therefore, did not occur as would have been predicted by these in vitro studies.…”

Section: Discussionmentioning

confidence: 96%

Hemolysis due to inadvertent hemodialysis against distilled water: Perils of bedside dialysate preparation

Pendergrast

Hladunewich²,

Richardson³

2006

Critical Care Medicine

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“…Literature data indicate that cells contain about 70% of water [Kageyama et al, 1989]. The human epidermis mostly consisting of keratinocytes contains approximately the same amount of total water [Warner et al, 1988;Caspers et al, 2003].…”

Section: Calculation Of Sar In Cell Layermentioning

confidence: 99%

Microscale temperature and SAR measurements in cell monolayer models exposed to millimeter waves

Алексеев

Sauleau

Page

et al. 2016

Bioelectromagnetics

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Due to shallow penetration of millimeter waves (MMW) and convection in liquid medium surrounding cells, the problem of accurate assessment of local MMW heating in in vitro experiments remains unsolved. Conventional dosimetric MMW techniques, such as infrared imaging or fiber optic (FO) sensors, face several inherent limits. Here we propose a methodology for accurate local temperature measurement and subsequent specific absorption rate (SAR) retrieval using microscale thermocouples (TC). SAR was retrieved by fitting the measured initial temperature rise to the numerical solution of an equivalent thermal model. It was found that the accuracy of temperature measurement depends on thermosensor size, that is, the smaller TC, the more accurate the temperature measurement. SAR determined using TC with lead diameters of 25 and 75 μm demonstrated 98.5% and 80.4% match with computed SAR, respectively. However, both TC provided the same temperature rises in long run (> 10 min). FO probe failed to measure adequately local heating both for short and long exposures due to the relatively large size of the probe sensor (400 μm) and time constant (0.6 s). Calculated SAR in the cell monolayer was almost two times lower than that in the surrounding liquid. It was shown that the impact of the cell monolayer on heating due to its small thickness (5 to 10 μm) can be considered as negligible. Moreover, we demonstrated the possibility of accurate measurement of MMW-induced thermal pulses (up to 10 °C) using 25 μm TC. Bioelectromagnetics. 38:11-21, 2017. © 2016 Wiley Periodicals, Inc.

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The maximum and minimum water content and cell volume of human erythrocytes in vitro

Cited by 21 publications

References 2 publications

Substratum‐dependent responses of ciliate assemblages to temperature: a natural experiment in Icelandic streams

Substratum‐dependent responses of ciliate assemblages to temperature: a natural experiment in Icelandic streams

Hemolysis due to inadvertent hemodialysis against distilled water: Perils of bedside dialysate preparation

Microscale temperature and SAR measurements in cell monolayer models exposed to millimeter waves

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