The Maturity of Tissue-Engineered Cartilage<i>In Vitro</i>Affects the Repairability for Osteochondral Defect

Jin, Cheng; Cho, Jae-Ho; Choi, Byung Hyune; Wang, Li Ming; Kim, Moon Suk; Park, Sora; Yoon, Jeong Ho; Oh, Hyun Ju; Min, Byoung‐Hyun

doi:10.1089/ten.tea.2010.0605

Cited by 46 publications

(27 citation statements)

References 24 publications

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“…Further maturation of the repair tissue is likely to occur over longer implantation times, which would enhance the delineation of the cartilage and bone interface. Two recent publications that used scaffolds combined with biological materials implanted into osteochondral defects showed mixed hyaline and fibrous repair cartilage below the osteochondral junction at comparable time points to those in the present study (32,33). However, both research groups evaluated the repair tissue at 12 wk, which showed segregation of the cartilage and bone layers, whereby the cartilage layer was similar in thickness to, and better resembled, the surrounding hyaline cartilage.…”

Section: Discussionsupporting

confidence: 63%

Bioinspired nanofibers support chondrogenesis for articular cartilage repair

Coburn¹,

Gibson

Monagle

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

198

135

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Articular cartilage repair remains a significant and growing clinical challenge with the aging population. The native extracellular matrix (ECM) of articular cartilage is a 3D structure composed of proteinaceous fibers and a hydrogel ground substance that together provide the physical and biological cues to instruct cell behavior. Here we present fibrous scaffolds composed of poly(vinyl alcohol) and the biological cue chondroitin sulfate with fiber dimensions on the nanoscale for application to articular cartilage repair. The unique, low-density nature of the described nanofiber scaffolds allows for immediate cell infiltration for optimal tissue repair. The capacity for the scaffolds to facilitate cartilage-like tissue formation was evaluated in vitro. Compared with pellet cultures, the nanofiber scaffolds enhance chondrogenic differentiation of mesenchymal stems cells as indicated by increased ECM production and cartilage specific gene expression while also permitting cell proliferation. When implanted into rat osteochondral defects, acellular nanofiber scaffolds supported enhanced chondrogenesis marked by proteoglycan production minimally apparent in defects left empty. Furthermore, inclusion of chondroitin sulfate into the fibers enhanced cartilage-specific type II collagen synthesis in vitro and in vivo. By mimicking physical and biological cues of native ECM, the nanofiber scaffolds enhanced cartilaginous tissue formation, suggesting their potential utility for articular cartilage repair.

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Section: Discussionsupporting

confidence: 63%

Bioinspired nanofibers support chondrogenesis for articular cartilage repair

Coburn¹,

Gibson

Monagle

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

198

135

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“…The quality of in vitro cartilage regeneration and its potential of endochondral ossification are key factors influencing the efficacy of osteochondral defect repair22. Therefore, gross and histological evaluations of BEC-vitro were first performed to investigate in vitro cartilage formation and its hypertrophic character.…”

Section: Resultsmentioning

confidence: 99%

Repair of osteochondral defects with in vitro engineered cartilage based on autologous bone marrow stromal cells in a swine model

Liu

Luo

et al. 2017

Sci Rep

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Functional reconstruction of large osteochondral defects is always a major challenge in articular surgery. Some studies have reported the feasibility of repairing articular osteochondral defects using bone marrow stromal cells (BMSCs) and biodegradable scaffolds. However, no significant breakthroughs have been achieved in clinical translation due to the instability of in vivo cartilage regeneration based on direct cell-scaffold construct implantation. To overcome the disadvantages of direct cell-scaffold construct implantation, the current study proposed an in vitro cartilage regeneration strategy, providing relatively mature cartilage-like tissue with superior mechanical properties. Our strategy involved in vitro cartilage engineering, repair of osteochondral defects, and evaluation of in vivo repair efficacy. The results demonstrated that BMSC engineered cartilage in vitro (BEC-vitro) presented a time-depended maturation process. The implantation of BEC-vitro alone could successfully realize tissue-specific repair of osteochondral defects with both cartilage and subchondral bone. Furthermore, the maturity level of BEC-vitro had significant influence on the repaired results. These results indicated that in vitro cartilage regeneration using BMSCs is a promising strategy for functional reconstruction of osteochondral defect, thus promoting the clinical translation of cartilage regeneration techniques incorporating BMSCs.

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“…The current hot spot is to find and use the degradable materials, or the combination of degradable (MR Functional Nano-HA [18], superparamagnetic iron doped HA nanoparticles [19], nanocomposite magnetic scaffolds [20], RGD [12], and homogeneously plasma [21], etc.) and non-degradable materials, to construct the 3d tissue engineering scaffold, providing the cells a 3d growth space, at the same time, the scaffold itself has the biological activity, which could induce the cell differentiation and vascular ingrowth [22,23]. With the development of 3D manufacturing technology, in recent years, the concept of constructing the 3D anatomical tissue engineering scaffold (ABTES), namely assisted by the characteristics, such as molding, manufacturing and biodegradation, of the biomedical polymer materials (abbrev.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the cell delivery-based bone repairing technology, which is currently available in clinic, is still limited to the repairing of focal lesions [5]. In recent years, the high polymer-inorganic substances has become the hotspot in the bone tissue engineering (BTE) research because of its biodegradability and easy 3D processing [6], Alothman et al [7] found that the composite of high-density polyethylene (HDPE)-hydroxyapatite (HA) exhibited the tensile strength as up to [22][23][24][25][26][27][28] MPa and the changes of HA content might adjust the characteristic of composite; Zein et al [8] found that the compressive strength of poly-e-caprolactone (PCL) could reach 4-77 MPa, and the yield strength cold reach 0.4-3.6 MPa when the pore size was 160-700 mm and the porosity was 48-77 %, which was much more suitable for the requirements of BTE.…”

Section: Introductionmentioning

confidence: 99%

Design, construction and mechanical testing of digital 3D anatomical data-based PCL–HA bone tissue engineering scaffold

Yao

Wei

Guo

et al. 2015

J Mater Sci: Mater Med

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The study aims to investigate the techniques of design and construction of CT 3D reconstructional data-based polycaprolactone (PCL)-hydroxyapatite (HA) scaffold. Femoral and lumbar spinal specimens of eight male New Zealand white rabbits were performed CT and laser scanning data-based 3D printing scaffold processing using PCL-HA powder. Each group was performed eight scaffolds. The CAD-based 3D printed porous cylindrical stents were 16 piece × 3 groups, including the orthogonal scaffold, the Pozi-hole scaffold and the triangular hole scaffold. The gross forms, fiber scaffold diameters and porosities of the scaffolds were measured, and the mechanical testing was performed towards eight pieces of the three kinds of cylindrical scaffolds, respectively. The loading force, deformation, maximum-affordable pressure and deformation value were recorded. The pore-connection rate of each scaffold was 100 % within each group, there was no significant difference in the gross parameters and micro-structural parameters of each scaffold when compared with the design values (P > 0.05). There was no significant difference in the loading force, deformation and deformation value under the maximum-affordable pressure of the three different cylinder scaffolds when the load was above 320 N. The combination of CT and CAD reverse technology could accomplish the design and manufacturing of complex bone tissue engineering scaffolds, with no significant difference in the impacts of the microstructures towards the physical properties of different porous scaffolds under large load.

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The Maturity of Tissue-Engineered CartilageIn VitroAffects the Repairability for Osteochondral Defect

Cited by 46 publications

References 24 publications

Bioinspired nanofibers support chondrogenesis for articular cartilage repair

Bioinspired nanofibers support chondrogenesis for articular cartilage repair

Repair of osteochondral defects with in vitro engineered cartilage based on autologous bone marrow stromal cells in a swine model

Design, construction and mechanical testing of digital 3D anatomical data-based PCL–HA bone tissue engineering scaffold

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