1994
DOI: 10.1006/dbio.1994.1042
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The Maternal Histone H1 Variant, H1M (B4 Protein), Is the Predominant H1 Histone in Xenopus Pregastrula Embryos

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Cited by 77 publications
(61 citation statements)
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“…We therefore conclude that xNAP-1 is a linker histone chaperone in Xenopus eggs. NAP-1 binding to linker histones may be specific to early embryos, because B4 is the linker histone subtype specific to early embryonic cells (13)(14)(15), and NAP-1 has been shown to be a chaperone for H2A and H2B in other cells (19,20). However, recent findings that histone H1 binding to chromatin is dynamic in mammalian culture cells (36,37) suggest a role for a similar linker histone chaperone to effect rapid exchange of histone H1 in somatic chromatin.…”
Section: Resultsmentioning
confidence: 99%
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“…We therefore conclude that xNAP-1 is a linker histone chaperone in Xenopus eggs. NAP-1 binding to linker histones may be specific to early embryos, because B4 is the linker histone subtype specific to early embryonic cells (13)(14)(15), and NAP-1 has been shown to be a chaperone for H2A and H2B in other cells (19,20). However, recent findings that histone H1 binding to chromatin is dynamic in mammalian culture cells (36,37) suggest a role for a similar linker histone chaperone to effect rapid exchange of histone H1 in somatic chromatin.…”
Section: Resultsmentioning
confidence: 99%
“…In Xenopus eggs, the core histones H2A-H2B and H3-H4 stored in the cytoplasm are associated with histone chaperones nucleoplasmin and N1͞N2, respectively (11,12). To investigate whether B4, the linker histone of Xenopus eggs (13)(14)(15), is found complexed to other proteins, egg extract was fractionated by gel filtration (Superose 12), and fractions were probed with Abs to B4. The result showed that B4 contained in egg extract was eluted considerably faster from the column than purified B4 in physiological buffer, whereas it was eluted at the same position as purified B4 in high-salt buffer containing 0.5 M KCl (Fig.…”
Section: Incorporation Of Linker Histones Into Dinucleosomesmentioning
confidence: 99%
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“…2B}. In this experiment the abundance of histone HI is compared with that of histone B4, because the amount of total B4 protein in the embryo remains constant through gastrulation (i.e., from fertilization to 14 hr postfertilization; Dworkin-Rastl et al 1994). However, because of the rapid increase in the number of embryonic nuclei, the amount of B4 per nucleus decreases during development (Dimitrov et al 1993).…”
Section: Histone Hi Inhibits the Selective Activation Of Chromosomal mentioning
confidence: 99%
“…The only exception was the maternal H1M protein of X . laevis (also referred to as B4; Smith et al, 1988;Dworkin-Rastl et al, 1994). The H1M (B4) protein shows not only the highest degree of sequence identity (47%) with the CS H1 protein, but also contains several of its unique amino acid residues (arrowheads; Fig.…”
Section: Ipakgaat-e-kqavks-~s:rtgdkk-r-r~-~-imentioning
confidence: 99%