The maternal coordinate system: Molecular-genetics of embryonic axis formation and patterning in the zebrafish

Fuentes, Ricardo; Tajer, Benjamin; Kobayashi, Manami; Pelliccia, José L; Langdon, Yvette; Abrams, Elliott W.; Mullins, Mary C.

doi:10.1016/bs.ctdb.2020.05.002

Cited by 20 publications

(25 citation statements)

References 242 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Main Textmentioning

confidence: 99%

“…Early embryonic zebrafish development is tightly regulated by various signaling cascades that should be sequentially activated in restricted domains and time windows [ 6 , 7 ]. The default state of the fertilized embryos is the ventral fate, determined by the expression of maternal genes related to Transforming growth factor beta (TGF-β) including radar, bone morphogenetic protein 2 (bmp2), bmp4 and bmp7 [ 7 – 9 ]. Ventral tissue specification during early embryonic development is abruptly interrupted at the onset of maternal-zygotic transition stage by the expression of various BMP antagonists such as bozozok, noggin and chordin .…”

Section: Main Textmentioning

confidence: 99%

“…Ventral tissue specification during early embryonic development is abruptly interrupted at the onset of maternal-zygotic transition stage by the expression of various BMP antagonists such as bozozok, noggin and chordin . The expression of these genes is triggered by the canonical Wnt (Wnt/β-catenin) and Fibroblast growth factor (FGF) signaling, and is restricted to putative organizer regions [ 7 , 10 ]. Formation of these organizer regions is a prerequisite for the subsequent anterior–posterior (AP) body axis specification.…”

Section: Main Textmentioning

confidence: 99%

See 2 more Smart Citations

Transgenic fluorescent zebrafish lines that have revolutionized biomedical research

et al. 2021

View full text Add to dashboard Cite

Since its debut in the biomedical research fields in 1981, zebrafish have been used as a vertebrate model organism in more than 40,000 biomedical research studies. Especially useful are zebrafish lines expressing fluorescent proteins in a molecule, intracellular organelle, cell or tissue specific manner because they allow the visualization and tracking of molecules, intracellular organelles, cells or tissues of interest in real time and in vivo. In this review, we summarize representative transgenic fluorescent zebrafish lines that have revolutionized biomedical research on signal transduction, the craniofacial skeletal system, the hematopoietic system, the nervous system, the urogenital system, the digestive system and intracellular organelles.

show abstract

Section: Main Textmentioning

confidence: 99%

Section: Main Textmentioning

confidence: 99%

Section: Main Textmentioning

confidence: 99%

See 1 more Smart Citation

Transgenic fluorescent zebrafish lines that have revolutionized biomedical research

et al. 2021

View full text Add to dashboard Cite

show abstract

“…The specification of dorsal axis in zebrafish also requires maternal ß-catenin signaling to trigger the expression of organizer genes (Kelly et al, 2000;Bellipanni et al, 2006;Fuentes et al, 2020). However, the involvement of upstream regulators has not been conclusively established.…”

Section: Maternal Contributions Of Dvl Proteins To Axis Patterning Anmentioning

confidence: 99%

Decoding Dishevelled-Mediated Wnt Signaling in Vertebrate Early Development

Shi

2020

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Dishevelled proteins are key players of Wnt signaling pathways. They transduce Wnt signals and perform cellular functions through distinct conserved domains. Due to the presence of multiple paralogs, the abundant accumulation of maternal transcripts, and the activation of distinct Wnt pathways, their regulatory roles during vertebrate early development and the mechanism by which they dictate the pathway specificity have been enigmatic and attracted much attention in the past decades. Extensive studies in different animal models have provided significant insights into the structure-function relationship of conserved Dishevelled domains in Wnt signaling and the implications of Dishevelled isoforms in early developmental processes. Notably, intra-and intermolecular interactions and Dishevelled dosage may be important in modulating the specificity of Wnt signaling. There are also distinct and redundant functions among Dishevelled isoforms in development and disease, which may result from differential spatiotemporal expression patterns and biochemical properties and post-translational modifications. This review presents the advances and perspectives in understanding Dishevelled-mediated Wnt signaling during gastrulation and neurulation in vertebrate early embryos.

show abstract

“…Maternal contribution is indispensable to drive early embryonic development, which is essentially “My mother made me do it” [ 1 ]. During oogenesis in zebrafish, meiosis temporally halts in a prolonged diplotene stage, when mRNAs and proteins are synthesized and stored for oocyte maturation, fertilization, and early development [ 2 , 3 , 4 , 5 , 6 , 7 , 8 ]. The gene products accumulated at this stage are termed maternal factors, which are generated by over half of the coding genes in zebrafish [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

A Time-Saving Strategy to Generate Double Maternal Mutants by an Oocyte-Specific Conditional Knockout System in Zebrafish

Zhang

Tarique

et al. 2021

Biology

View full text Add to dashboard Cite

Maternal products are those mRNAs and proteins deposited during oogenesis, which play critical roles in controlling oocyte formation, fertilization, and early embryonic development. However, loss-of-function studies for these maternal factors are still lacking, mainly because of the prolonged period of transgenerational screening and technical barriers that prevent the generation of maternal (M) and maternal and zygotic (MZ) mutant embryos. By the transgenic expression of multiple sgRNAs targeting a single gene of interest in the background of a transgenic line Tg(zpc:zcas9) with oocyte-specific cas9 expression, we have successfully obtained maternal or maternal–zygotic mutant for single genes in F1 embryos. In this work, we tandemly connected a maternal GFP marker and eight sgRNA expression units to target dvl2 and dvl3a simultaneously and introduced this construct to the genome of Tg(zpc:zcas9) by meganuclease I-Sce I. As expected, we confirmed the existence of Mdvl2;Mdvl3a embryos with strong defective convergence and extension movement during gastrulation among outcrossed GFP positive F1 offspring. The MZdvl2;MZdvl3a embryos were also obtained by crossing the mutant carrying mosaic F0 female with dvl2+/−;dvl3a−/− male fish. This proof-of-principle thus highlights the potential of this conditional knockout strategy to circumvent the current difficulty in the study of genes with multiple functionally redundant paralogs.

show abstract

The maternal coordinate system: Molecular-genetics of embryonic axis formation and patterning in the zebrafish

Cited by 20 publications

References 242 publications

Transgenic fluorescent zebrafish lines that have revolutionized biomedical research

Transgenic fluorescent zebrafish lines that have revolutionized biomedical research

Decoding Dishevelled-Mediated Wnt Signaling in Vertebrate Early Development

A Time-Saving Strategy to Generate Double Maternal Mutants by an Oocyte-Specific Conditional Knockout System in Zebrafish

Contact Info

Product

Resources

About