The MAP30 protein from bitter gourd (Momordica charantia) seeds promotes apoptosis in liver cancer cells in vitro and in vivo

Fang, Evandro Fei; Zhang, Chris Zhi Yi; Wong, Jack Ho; Shen, Jia; Li, Chuanhao; Ng, Tzi Bun

doi:10.1016/j.canlet.2012.05.005

Cited by 98 publications

(79 citation statements)

References 40 publications

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“…Some pharmacological and safety studies of this herb have been carried out 16,17 . In addition to hypoglycaemic activity, M.charantia has been shown to have antioxidant [18][19][20] , anti-tumour [21][22][23][24][25] , neuroprotective 26 , anti-inflammatory [27][28][29] and antimicrobial activity 30,31 . It has a resistance modifying effect for aminoglycosides against methicillin-resistant Staphylococcus aureus 32 .…”

Section: Introductionmentioning

confidence: 99%

In vivo hypoglycemic effect of methanolic fruit extract of <i>Momordica charantia L</i>

Nkambo¹,

Anyama²,

Onegi³

2014

Afr H. Sci.

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Background: Momordica charantia L. is a medicinal plant commonly used in the management of diabetes mellitus. Objectives: We investigated the blood glucose lowering effect of the methanolic fruit extract of the Ugandan variety of M. charantia L. in alloxan-induced diabetic albino rats. Methods: 500g of M. charantia powder were macerated in methanol and the extract administered to two groups of alloxaninduced diabetic rats. The first group received 125mg/kg, the second 375mg/kg and a third group 7mg/ kg of metformin. A fourth group received 1ml normal saline. Fasting blood glucose (FBG) levels were measured at 0.5,1,2,3,5,8 and 12 hours and compared using one-way ANOVA. Results: There was an initial rise in FBG for 1 hour after administration of extracts followed by steep reductions. Significant reduction in FBG occurred at 2 hours for 125mg/kg of extract (-3.2%, 313±25.9 to 303±25.0mg/dL, p = 0.049), 375mg/ kg of extract (-3.9%, 356±19.7 to 342±20.3mg/dL, p = 0.001), and metformin (-2.6%, 344±21.7 to 335±21.1mg/dL, p = 0.003) when compared to normal saline. The maximum percentage reduction in FBG by both extracts occurred between 3 and 12 hours post dose. Conclusions: The methanolic fruit extract of M. charantia exhibits dose dependent hypoglycaemic activity in vivo.

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Section: Introductionmentioning

confidence: 99%

In vivo hypoglycemic effect of methanolic fruit extract of <i>Momordica charantia L</i>

Nkambo¹,

Anyama²,

Onegi³

2014

Afr H. Sci.

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“…The apoptosis-promoting and cell cycle-arresting abilities of MAP30 were identified in previous studies (14,(28)(29)(30). MAP30 was considered to cleave adenine-ribose glycosidic bonds by targeting the conserved host protein synthesis machinery, and prevent the elongation of DNA/RNA, resulting in DNA replication arrest in S-phase (3,16,17).…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, development of novel and effective therapeutic agents is highly desirable. Although previous studies have identified that MAP30 possesses antitumor effects in various tumor cells, including glioblastoma cells, the underlying molecular mechanism of MAP30 on glioblastoma cells has not yet been elucidated (4,6,(12)(13)(14)16,21,22). The results of the present study demonstrated that MAP30 displayed significant inhibition of proliferation, invasion and migration on U87 and U251 cell lines in a dose-and time-dependent manner.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies indicated that MAP30 exhibits a variety of anti-infection, anti-diabetic, antiviral and antitumor bioactive effects (4)(5)(6)(7)(8)(9)(10). The antitumor ability of MAP30 has been the subject of previous studies (11)(12)(13)(14)(15). However, certain researchers have already noted the effects of MAP30 on the inhibition of U87 cells in screening for anticancer effects (3,7,16,17).…”

Section: Introductionmentioning

confidence: 99%

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MAP30 promotes apoptosis of U251 and U87 cells by suppressing the LGR5 and Wnt/β-catenin signaling pathway, and enhancing Smac expression

et al. 2018

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Abstract. Significant antitumor activity of Momordica anti-human immunodeficiency virus protein of 30 kDa (MAP30) purified from Momordica charantia has been the subject of previous research. However, the effective mechanism of MAP30 on malignant glioma cells has not yet been clarified. The aim of the present study was to investigate the effects and mechanism of MAP30 on U87 and U251 cell lines. A Cell Counting Kit-8 assay, wound healing assay and Transwell assay were used to detect the effects on U87 and U251 cells treated with different concentrations of MAP30 (0.5, 1, 2, 4, 8 and 16 µM) over different periods of time. Proliferation, migration and invasion of each cell line were markedly inhibited by MAP30 in a dose-and time-dependent manner. Flow cytometry and fluorescence staining demonstrated that apoptosis increased and the cell cycle was arrested in S-phase in the two investigated cell lines following MAP30 treatment. Western blot analysis demonstrated that leucine-rich-repeat-containing G-protein-coupled receptor 5 (LGR5) expression and key proteins in the Wnt/β-catenin signaling pathway were apparently decreased, whereas second mitochondria-derived activator of caspase (Smac) protein expression significantly increased with MAP30 treatment in the same manner. These results suggest that MAP30 markedly induces apoptosis in U87 and U251 cell lines by suppressing LGR5 and the Wnt/β-catenin signaling pathway, and enhancing Smac expression in a dose-and time-dependent manner.

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“…In one study, a water extract blocked the growth of rat prostate carcinoma; another study reported that a hot water extract of the entire plant inhibited the development of mammary tumors in mice. Numerous in vitro studies have also demonstrated the anti-cancerous and anti-leukemic activity of bitter gourd against numerous cell lines, including liver cancer, human leukemia, melanoma and solid sarcomas [34][35]. The other realm showing that bitter gourd is as an effective immunomodulator.…”

Section: Anti-tumor Propertiesmentioning

confidence: 99%

Nutritional, Pharmacological and Medicinal Properties of Momordica Charantia

Kumar¹,

Nallamuthu²

2015

IJNFS

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Abstract:Momordica charantia L. (Bitter gourd) is a flowering vine in the family of Cucurbitaceae. It contains an array of novel and biologically active phytochemicals including triterpenes, proteins and steroids. Medicinally, the plant has a long history of use by the indigenous people as a folk medicine. Bitter gourd is often used in Chinese cooking for its bitter flavor, typically in stir-fries, soups, and also as tea. Pakistan, Philippines, Panama and Nepal also use this bitter vegetable for culinary purposes in addition to India. Several medicinal properties of the bitter gourd have been studied by various researchers, such as anti-diabetic, anti-ulcerogenic, anti-mutagenic, antioxidant, anti-tumour, anti-lipolytic, analgesic, abortifacient, anti-viral, hypoglycemic and immunomodulatory. In vitro studies reveals that the bitter gourd proteins (α-and β-monorcharin) have inhibitory effect against HIV virus, and leaf extracts have broad-spectrum anti-microbial activity as well. Many in vivo studies have demonstrated the relatively low toxicity of all parts of the bitter gourd plant when ingested orally. This review also addresses taxonomy, phytochemical, culinary practices and pharmacological properties in detail. Over the years scientists have verified many of the traditional uses of this bitter plant that continues to be an important natural remedy in herbal medicine systems. Bitter gourd products such as concentrated fruit and seed extracts can be found in capsules and tablets, as well as in whole herb/vine powder forms and these supplements are becoming more widely available in many countries nowadays as prophylactic or therapeutic agents.

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The MAP30 protein from bitter gourd (Momordica charantia) seeds promotes apoptosis in liver cancer cells in vitro and in vivo

Cited by 98 publications

References 40 publications

In vivo hypoglycemic effect of methanolic fruit extract of <i>Momordica charantia L</i>

In vivo hypoglycemic effect of methanolic fruit extract of <i>Momordica charantia L</i>

MAP30 promotes apoptosis of U251 and U87 cells by suppressing the LGR5 and Wnt/β-catenin signaling pathway, and enhancing Smac expression

Nutritional, Pharmacological and Medicinal Properties of Momordica Charantia

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