The many faces of ribosome translocation along the mRNA: reading frame maintenance, ribosome frameshifting and translational bypassing

Poulis, Panagiotis; Peske, Frank; Rodnina, Marina V.

doi:10.1515/hsz-2023-0142

Cited by 4 publications

(2 citation statements)

References 135 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Translocation is in large part mediated by the GTPase activity of elongation factor G (EF-G) in prokaryotes ( Rodnina et al, 1997 ; Liu et al, 2011 ; Poulis et al, 2023 ) or eEF2 in eukaryotes ( Kaul et al, 2011 ; Djumagulov et al, 2021 ; Milicevic et al, 2024 ). EF-G binds to the A-site to transfer deacylated P/E-site tRNA and peptidyl A/P-site tRNA to the E and P-sites, respectively.…”

Section: Translocation Through the Ribosomementioning

confidence: 99%

Tuning tRNAs for improved translation

Weiss,

Decker,

Bolano

et al. 2024

Front. Genet.

View full text Add to dashboard Cite

Transfer RNAs have been extensively explored as the molecules that translate the genetic code into proteins. At this interface of genetics and biochemistry, tRNAs direct the efficiency of every major step of translation by interacting with a multitude of binding partners. However, due to the variability of tRNA sequences and the abundance of diverse post-transcriptional modifications, a guidebook linking tRNA sequences to specific translational outcomes has yet to be elucidated. Here, we review substantial efforts that have collectively uncovered tRNA engineering principles that can be used as a guide for the tuning of translation fidelity. These principles have allowed for the development of basic research, expansion of the genetic code with non-canonical amino acids, and tRNA therapeutics.

show abstract

Section: Translocation Through the Ribosomementioning

confidence: 99%

Tuning tRNAs for improved translation

Weiss,

Decker,

Bolano

et al. 2024

Front. Genet.

View full text Add to dashboard Cite

show abstract

“…To complement our chromosomal UUA analysis, we have experimentally investigated the proposed Hau3 bypassing. Bypassing is known to be required in the decoding of E. coli phage T4 [ 22 , 53 , 54 ]. Fifty nucleotides are bypassed with 40% efficiency with bypassing of the 3’ part of the coding gap involving the scanning of each overlapping triplet in a process that uses 2 GTP per nucleotide bypassed [ 55 , 56 ].…”

Section: Introductionmentioning

confidence: 99%

Streptomyces rare codon UUA: from features associated with 2 adpA related locations to candidate phage regulatory translational bypassing

Antonov,

O’Loughlin,

Gorohovski

et al. 2023

RNA Biology

View full text Add to dashboard Cite

In Streptomyces species, the cell cycle involves a switch from an early and vegetative state to a later phase where secondary products including antibiotics are synthesized, aerial hyphae form and sporulation occurs. AdpA, which has two domains, activates the expression of numerous genes involved in the switch from the vegetative growth phase. The adpA mRNA of many Streptomyces species has a UUA codon in a linker region between 5’ sequence encoding one domain and 3’ sequence encoding its other and C-terminal domain. UUA codons are exceptionally rare in Streptomyces, and its functional cognate tRNA is not present in a fully modified and acylated form, in the early and vegetative phase of the cell cycle though it is aminoacylated later. Here, we report candidate recoding signals that may influence decoding of the linker region UUA. Additionally, a short ORF 5’ of the main ORF has been identified with a GUG at, or near, its 5’ end and an in-frame UUA near its 3’ end. The latter is commonly 5 nucleotides 5’ of the main ORF start. Ribosome profiling data show translation of that 5’ region. Ten years ago, UUA-mediated translational bypassing was proposed as a sensor by a Streptomyces phage of its host’s cell cycle stage and an effector of its lytic/lysogeny switch. We provide the first experimental evidence supportive of this proposal.

show abstract

On the response of elongating ribosomes to forces opposing translocation

Moore

2024

Biophysical Journal

View full text Add to dashboard Cite

The many faces of ribosome translocation along the mRNA: reading frame maintenance, ribosome frameshifting and translational bypassing

Cited by 4 publications

References 135 publications

Tuning tRNAs for improved translation

Tuning tRNAs for improved translation

Streptomyces rare codon UUA: from features associated with 2 adpA related locations to candidate phage regulatory translational bypassing

On the response of elongating ribosomes to forces opposing translocation

Contact Info

Product

Resources

About