The many faces of hematopoietic stem cell heterogeneity

Crisan, Mihaela; Dzierzak, Elaine

doi:10.1242/dev.114231

Cited by 83 publications

(68 citation statements)

References 118 publications

(162 reference statements)

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“…We do not intend here to provide detailed overview of non-human models, as this extensive topic has been reviewed recently by us and by others Kim et al, 2014;Ciau-Uitz et al, 2016;Kauts et al, 2016;Robertson et al, 2016;Crisan and Dzierzak, 2016). Owing to the high accessibility of model organisms for experimentation and the genetic variability and rarity of human embryonic material, the analysis of human haematopoietic development has lagged behind these wellestablished experimental models and for a long time was limited to immunohistological and in vitro studies.…”

Section: Introductionmentioning

confidence: 99%

Human haematopoietic stem cell development: from the embryo to the dish

et al. 2017

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Haematopoietic stem cells (HSCs) emerge during embryogenesis and give rise to the adult haematopoietic system. Understanding how early haematopoietic development occurs is of fundamental importance for basic biology and medical sciences, but our knowledge is still limited compared with what we know of adult HSCs and their microenvironment. This is particularly true for human haematopoiesis, and is reflected in our current inability to recapitulate the development of HSCs from pluripotent stem cells in vitro. In this Review, we discuss what is known of human haematopoietic development: the anatomical sites at which it occurs, the different temporal waves of haematopoiesis, the emergence of the first HSCs and the signalling landscape of the haematopoietic niche. We also discuss the extent to which in vitro differentiation of human pluripotent stem cells recapitulates bona fide human developmental haematopoiesis, and outline some future directions in the field.

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Section: Introductionmentioning

confidence: 99%

Human haematopoietic stem cell development: from the embryo to the dish

et al. 2017

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“…Several studies have shown that contact between HSCs and stromal cells is important for maintaining HSC function [48,49]. The stromal cells from bone marrow include osteoblasts, macrophages, endothelial cells, and mesenchymal cells [50,51]. Mesenchymal cells are multipotent progenitor cells that can differentiate into mesenchymal cells, such as osteoblasts, adipocytes, and chondrocytes, and also have the potential for differentiation into cells such as neurons and lung cells [52].…”

Section: Stem Cell Culture Methodsmentioning

confidence: 99%

Traditional and Advanced Cell Cultures in Hematopoietic Stem Cell Studies

Ribeiro-Filho

Levy

Ruiz

et al. 2019

Cells

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Hematopoiesis is the main function of bone marrow. Human hematopoietic stem and progenitor cells reside in the bone marrow microenvironment, making it a hotspot for the development of hematopoietic diseases. Numerous alterations that correspond to disease progression have been identified in the bone marrow stem cell niche. Complex interactions between the bone marrow microenvironment and hematopoietic stem cells determine the balance between the proliferation, differentiation and homeostasis of the stem cell compartment. Changes in this tightly regulated network can provoke malignant transformation. However, our understanding of human hematopoiesis and the associated niche biology remains limited due to accessibility to human material and the limits of in vitro culture models. Traditional culture systems for human hematopoietic studies lack microenvironment niches, spatial marrow gradients, and dense cellularity, rendering them incapable of effectively translating marrow physiology ex vivo. This review will discuss the importance of 2D and 3D culture as a physiologically relevant system for understanding normal and abnormal hematopoiesis.

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“…With the discovery of markers that can subdivide the niches, more exciting biology awaits. For example, HSCs are heterogenous in their cell cycle status, marker expression, lineage output and response to extrinsic signals . This raises the question as to whether there is heterogeneity within the niches.…”

Section: Perspectivesmentioning

confidence: 99%

Extrinsic regulation of hematopoietic stem cells in development, homeostasis and diseases

Lee

Decker

Lee

et al. 2017

WIREs Developmental Biology

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Lifelong generation of blood and immune cells depends on hematopoietic stem cells (HSCs). Their function is precisely regulated by complex molecular networks that integrate and respond to ever changing physiological demands of the body. Over the past several years, significant advances have been made in understanding the extrinsic regulation of HSCs during development and in homeostasis. Propelled by technical advances in the field, the cellular and molecular components of the microenvironment that support HSCs in vivo are emerging. In addition, the interaction of HSCs with their niches is appreciated as a critical contributor to the pathogenesis of a number of hematological disorders. Here, we review these advances in detail and highlight the extrinsic regulation of HSCs in the context of development, homeostasis and diseases.

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The many faces of hematopoietic stem cell heterogeneity

Cited by 83 publications

References 118 publications

Human haematopoietic stem cell development: from the embryo to the dish

Human haematopoietic stem cell development: from the embryo to the dish

Traditional and Advanced Cell Cultures in Hematopoietic Stem Cell Studies

Extrinsic regulation of hematopoietic stem cells in development, homeostasis and diseases

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