I. The compositions of the fatty acids in ryegrass were determined in fresh samples, and in samples dried at room temperature for 26 h, at 50' for 18 h or at 100' for 12 h. The drying of grass resulted in a small increase in palmitic acid and a decrease in linolenic acid in the total fatty acids.2. Samples of grass dried at 50' and IOOO were stored at three relative humidities (rh < 3 yo, 47 yo and 80 %) for 13 months.3. There were marked changes in the samples stored at 80% rh, with an onset of mould growth and a loss of dry matter. The samples stored at lower humidities had no mould.4. There were two types of change in the fatty acids during storage. The deterioration brought about by mould was accompanied by a decrease in the concentration of linolenic acid and an increase in the concentrations of oleic and linoleic acids. The other type of change observed at the lower humidities resulted in a decrease in the content of linolenic and an increase in the content of palmitic, and did not affect the amounts of oleic and linoleic cells.5. There was little difference between the changes that occurred in the composition of the total fatty acids of the grass dried at 50' and of that dried at 100". The changes that were at all significant appeared to occur less rapidly, particularly in the early months of storage, in the grass dried at the higher temperature for the shorter time.The successful preservation of dried forages in an intact form is of economic importance, and various aspects of this problem were studied intermittently at this Institute and elsewhere, particularly during the Second World War and immediately afterwards. On long storage in this country feeding-stuffs can be affected by moulding, and this aspect of the problem has received much attention (cf. Wright, 1941). The onset of moulding is related to the moisture content of the feeding-stuff and the latter was shown to be a direct function of the relative humidity (rh) of the atmosphere (Snow, Crichton & Wright, 1944a, b), but the emphasis was usually on prevention of moulding rather than on the changes that occurred in the composition of the material studied. The problem was reinvestigated by Waite (1949), who pointed out that the development of moulding depended on the storage temperature and that the critical humidity derived from laboratory experiments did not necessarily apply under practical conditions where the temperature was normally lower. Changes in the content of some components of the feeding-stuffs were investigated by Snow, Smith & Wright (1945) and by Snow & Wright (1945), and deterioration caused by agents other than mould was also considered.Lipid is a minor but important constituent of roughages (Garton, 1960), but any serious investigation of changes in this constituent during storage had to await the development of the new chromatographic techniques. Collins & Shorland ( I 944-5) studied the change that occurred in the total amounts of fatty acids and phospholipids in grass during drying and storage periods of relatively short dur...