The Management of Craniofacial Microsomia in a Craniofacial Center

Hutagalung, Magda Rosalina; Tan, Andy

doi:10.1097/scs.0000000000007105

Cited by 1 publication

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“…For example, DO is now used to promote bone in a variety of conditions with congenital skeletal deficiency (e.g. Treacher Collins Syndrome, Pierre Robin Sequence, and hemifacial microsomia), resulting in reduced rates of airway obstruction and tracheostomy, and improved health-related quality of life 2 – 5 .…”

Section: Introductionmentioning

confidence: 99%

Denervation during mandibular distraction osteogenesis results in impaired bone formation

Tevlin

Chen

Januszyk

et al. 2023

Sci Rep

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Mandibular distraction osteogenesis (DO) is mediated by skeletal stem cells (SSCs) in mice, which enact bone regeneration via neural crest re-activation. As peripheral nerves are essential to progenitor function during development and in response to injury, we questioned if denervation impairs mandibular DO. C57Bl6 mice were divided into two groups: DO with a segmental defect in the inferior alveolar nerve (IAN) at the time of mandibular osteotomy (“DO Den”) and DO with IAN intact (“DO Inn”). DO Den demonstrated significantly reduced histological and radiological osteogenesis relative to DO Inn. Denervation preceding DO results in reduced SSC amplification and osteogenic potential in mice. Single cell RNA sequencing analysis revealed that there was a predominance of innervated SSCs in clusters dominated by pathways related to bone formation. A rare human patient specimen was also analyzed and suggested that histological, radiological, and transcriptional alterations seen in mouse DO may be conserved in the setting of denervated human mandible distraction. Fibromodulin (FMOD) transcriptional and protein expression were reduced in denervated relative to innervated mouse and human mandible regenerate. Finally, when exogenous FMOD was added to DO-Den and DO-Inn SSCs undergoing in vitro osteogenic differentiation, the osteogenic potential of DO-Den SSCs was increased in comparison to control untreated DO-Den SSCs, modeling the superior osteogenic potential of DO-Inn SSCs.

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Section: Introductionmentioning

confidence: 99%