The Mammalian Reticulocyte

Lowenstein, Leah M.

doi:10.1016/s0074-7696(08)62730-8

Cited by 65 publications

(15 citation statements)

References 225 publications

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“…Our data are in agreement with the idea that down-regulation requires active protein synthesis [1,2], The changes that occur in plasma membranes and intracel lular organelles [18,19] during red cell matu ration can then explain the mature RBC inha bility to down-regulate insulin receptors.…”

Section: Discussionsupporting

confidence: 91%

Insulin Receptor Regulation in Human Mature Red Cells in vitro

Gherzi¹,

Andraghetti²,

Adezati³

et al. 1985

Horm Res

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We have studied the ability of mature red cells to regulate the number and affinity of their insulin receptors, in vitro. Our data show that mature red cells are not able to change either the number and the affinity of their insulin receptors, after preincubation with high concentrations of insulin alone or insulin and glucose. We conclude that mature red cells possess an insulin receptor system not completely similar to that of major target cells such as hepatocytes and adipocytes, and therefore we suggest some criticism in evaluating these cells in clinical studies, regarding the insulin receptor status.

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Section: Discussionsupporting

confidence: 91%

Insulin Receptor Regulation in Human Mature Red Cells in vitro

Gherzi¹,

Andraghetti²,

Adezati³

et al. 1985

Horm Res

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“…[27] While our study measured unstained reticulocytes in a wet preparation, smears of post-vitally stained reticulocytes also show a noticeable reduction in cellular diameter. It is a bit surprising that Heilmeyer neglected to reflect the obvious change in reticulocyte size in the figure he used illustrate his classification system.…”

Section: Discussionmentioning

confidence: 69%

Significant Biochemical, Biophysical and Metabolic Diversity in Circulating Human Cord Blood Reticulocytes

et al. 2013

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BackgroundThe transition from enucleated reticulocytes to mature normocytes is marked by substantial remodeling of the erythrocytic cytoplasm and membrane. Despite conspicuous changes, most studies describe the maturing reticulocyte as a homogenous erythropoietic cell type. While reticulocyte staging based on fluorescent RNA stains such as thiazole orange have been useful in a clinical setting; these ‘sub-vital’ stains may confound delicate studies on reticulocyte biology and may preclude their use in heamoparasite invasion studies.Design and MethodsHere we use highly purified populations of reticulocytes isolated from cord blood, sorted by flow cytometry into four sequential subpopulations based on transferrin receptor (CD71) expression: CD71high, CD71medium, CD71low and CD71negative. Each of these subgroups was phenotyped in terms of their, morphology, membrane antigens, biomechanical properties and metabolomic profile.ResultsSuperficially CD71high and CD71medium reticulocytes share a similar gross morphology (large and multilobular) when compared to the smaller, smooth and increasingly concave reticulocytes as seen in the in the CD71low and CD71negativesamples. However, between each of the four sample sets we observe significant decreases in shear modulus, cytoadhesive capacity, erythroid receptor expression (CD44, CD55, CD147, CD235R, and CD242) and metabolite concentrations. Interestingly increasing amounts of boric acid was found in the mature reticulocytes.ConclusionsReticulocyte maturation is a dynamic and continuous process, confounding efforts to rigidly classify them. Certainly this study does not offer an alternative classification strategy; instead we used a nondestructive sampling method to examine key phenotypic changes of in reticulocytes. Our study emphasizes a need to focus greater attention on reticulocyte biology.

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“…The erythrocyte has a lifespan of approximately 120 days in the circulation, which means that the parameter MCH is a measure of the of the iron availability for the erythropoiesis during this time period. In contrast to this, CHr gives a snapshot of the iron availability for erythropoiesis, since the lifespan of the reticulocyte is just 1-2 days [3]. Thus, a decrease in reticulocyte hemoglobin content precedes a similar decrease in erythrocytes and is an early and reliable indicator of iron deficiency anemia [4, 5].…”

Section: Introductionmentioning

confidence: 99%

Comparative Evaluation of the Reticulocyte Hemoglobin Content Assay When Screening for Iron Deficiency in Elderly Anemic Patients

Karlsson

2011

Anemia

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The aim of this study was to evaluate sensitivity and specificity for reticulocyte hemoglobin content (CHr) compared to other hematimetric and biochemical iron parameters, in particular, mean corpuscular hemoglobin (MCH), when screening for iron deficiency in elderly anemic patients. Bone marrow staining negative for iron was used as the gold standard criterion for iron deficiency anemia (IDA). Sensitivity and specificity for CHr, soluble transferrin receptor (sTfR), soluble transferrin receptor/log ferritin (TfR-F index), ferritin, MCH, and transferrin saturation were determined. The best cut-off point for CHr was 30.5 pg corresponding to a sensitivity and specificity of 93% and 69% for IDA, respectively. For MCH, a sensitivity of 93% and a specificity of 86%, respectively, correspond to an optimal cut-off of 28.5 pg. Analysis of CHr was not superior to MCH with respect to sensitivity and specificity when screening for IDA in elderly anemic patients.

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The Mammalian Reticulocyte

Cited by 65 publications

References 225 publications

Insulin Receptor Regulation in Human Mature Red Cells in vitro

Insulin Receptor Regulation in Human Mature Red Cells in vitro

Significant Biochemical, Biophysical and Metabolic Diversity in Circulating Human Cord Blood Reticulocytes

Comparative Evaluation of the Reticulocyte Hemoglobin Content Assay When Screening for Iron Deficiency in Elderly Anemic Patients

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