The malignant social network

Hale, James S.; Li, Meizhang; Lathia, Justin D.

doi:10.4161/cam.21294

Cited by 43 publications

(17 citation statements)

References 146 publications

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“…The traditional two-dimensional (2D) cell culture systems are adequate to study cancer cells in vitro , but cannot completely simulate the in vivo cellular environment. This important difference in the cellular surroundings [9] may influence CSC properties and prevent their differentiation [10].…”

Section: Introductionmentioning

confidence: 99%

Breast Cancer Stem Cell Culture and Enrichment Using Poly(ε-Caprolactone) Scaffolds

et al. 2016

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Abstract:The cancer stem cell (CSC) population displays self-renewal capabilities, resistance to conventional therapies, and a tendency to post-treatment recurrence. Increasing knowledge about CSCs' phenotype and functions is needed to investigate new therapeutic strategies against the CSC population. Here, poly(ε-caprolactone) (PCL), a biocompatible polymer free of toxic dye, has been used to fabricate scaffolds, solid structures suitable for 3D cancer cell culture. It has been reported that scaffold cell culture enhances the CSCs population. A RepRap BCN3D+ printer and 3 mm PCL wire were used to fabricate circular scaffolds. PCL design and fabrication parameters were first determined and then optimized considering several measurable variables of the resulting scaffolds. MCF7 breast carcinoma cell line was used to assess scaffolds adequacy for 3D cell culture. To evaluate CSC enrichment, the Mammosphere Forming Index (MFI) was performed in 2D and 3D MCF7 cultures. Results showed that the 60˝scaffolds were more suitable for 3D culture than the 45˝and 90˝ones. Moreover, 3D culture experiments, in adherent and non-adherent conditions, showed a significant increase in MFI compared to 2D cultures (control). Thus, 3D cell culture with PCL scaffolds could be useful to improve cancer cell culture and enrich the CSCs population.

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Section: Introductionmentioning

confidence: 99%

Breast Cancer Stem Cell Culture and Enrichment Using Poly(ε-Caprolactone) Scaffolds

et al. 2016

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“…Cell density is closely dependent on cell adhesion and cell-to-cell communication. Our group and many other researchers have previously outlined the importance of adhesion and cell communication molecules in CSC maintenance and expansion (20,(68)(69)(70)(71). However, in an experimental setting, cell density is often overlooked and poorly controlled for across studies in CSC field.…”

Section: Discussionmentioning

confidence: 99%

High‐Throughput Automated Single‐Cell Imaging Analysis Reveals Dynamics of Glioblastoma Stem Cell Population During State Transition

et al. 2019

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Cancer stem cells (CSCs) are a heterogeneous and dynamic self‐renewing population that stands at the top of tumor cellular hierarchy and contribute to tumor recurrence and therapeutic resistance. As methods of CSC isolation and functional interrogation advance, there is a need for a reliable and accessible quantitative approach to assess heterogeneity and state transition dynamics in CSCs. We developed a high‐throughput automated single cell imaging analysis (HASCIA) approach for the quantitative assessment of protein expression with single‐cell resolution and applied the method to investigate spatiotemporal factors that influence CSC state transition using glioblastoma (GBM) CSCs (GSCs) as a model system. We were able to validate the quantitative nature of this approach through comparison of the protein expression levels determined by HASCIA to those determined by immunoblotting. A virtue of HASCIA was exemplified by detection of a subpopulation of SOX2‐low cells, which expanded in fraction size during state transition. HASCIA also revealed that GSCs were committed to loose stem cell state at an earlier time point than the average SOX2 level decreased. Functional assessment of stem cell frequency in combination with the quantification of SOX2 expression by HASCIA defined a stable cutoff of SOX2 expression level for stem cell state. We also developed an approach to assess local cell density and found that denser monolayer areas possess higher average levels of SOX2, higher cell diversity, and a presence of a sub‐population of slowly proliferating SOX2‐low GSCs. HASCIA is an open source software that facilitates understanding the dynamics of heterogeneous cell population such as that of GSCs and their progeny. It is a powerful and easy‐to‐use image analysis and statistical analysis tool available at https://hascia.lerner.ccf.org. © 2019 International Society for Advancement of Cytometry

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“…This was more apparent in CSCs and vascular niches. As previous studies have reported that CSCs or vascular niches may be the base for metastasis and network-like information export and exchange ( 15 , 49 , 50 ), it was hypothesized that cellular communication would be more strongly observed near these niches in vivo . However, the transmission of information to the distant areas of the tissues may still need to occur through microtubule-like channels, cell-associated cords or tumor angiogenesis.…”

Section: Discussionmentioning

confidence: 99%

Cellular communication promotes mammosphere growth and collective invasion through microtubule‑like structures and angiogenesis

et al. 2018

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Networks of nanotubes and microtubules are highly valued in cellular communication, and collective cancer movement has been revealed to be associated with cell information exchange. In the present study, cellular communication was demonstrated to participate in mammosphere growth, differentiation and collective invasion. By promoting differentiation, networks of cells and microtubule-like structures were verified. Analyses of cell cycle progression, stemness markers and gene expression indicated that mammospheres had collective characteristics of stemness and differentiation. Invasion assays revealed that networks of microtubule-like structures promoted collective invasion. Conversely, using anti-angiogenic intervention, the growth of stem-like mammospheres and cellular communication links were effectively inhibited. In vivo experiments revealed that cellular communication promoted tumor growth and metastasis through the formation of nodular fusion, cluttered microtubule-like structures and cancer stem cells, as well as vascular niches. In conclusion, the present results demonstrated that a network of cells and structures were largely present in mammosphere cellular communication in vitro and in vivo. Therefore, blocking cellular communication may prove beneficial in halting the progression of mammary tumors.

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The malignant social network

Cited by 43 publications

References 146 publications

Breast Cancer Stem Cell Culture and Enrichment Using Poly(ε-Caprolactone) Scaffolds

Breast Cancer Stem Cell Culture and Enrichment Using Poly(ε-Caprolactone) Scaffolds

High‐Throughput Automated Single‐Cell Imaging Analysis Reveals Dynamics of Glioblastoma Stem Cell Population During State Transition

Cellular communication promotes mammosphere growth and collective invasion through microtubule‑like structures and angiogenesis

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