The male gamete membrane protein DMP9/DAU2 is required for double fertilization in flowering plants

Takahashi, Taro; Mori, Toshiyuki; Uéda, Kenji; Yamada, Lixy; Nagahara, Shiori; Higashiyama, Tetsuya; Sawada, Hitoshi; Igawa, Tomoko

doi:10.1242/dev.170076

Cited by 68 publications

(49 citation statements)

References 31 publications

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“…Double fertilization in vascular plants involves three cellular fusions and three nuclear fusions as sperm fertilize egg sacs (Maruyama et al ., ; Dresselhaus et al ., ). Omics and genetic approaches have successfully discovered critical players in which mutations abolish nuclear/organellar fusions during plant fertilization, and have revealed how intricately these events relate to embryo and endosperm development (Maruyama et al ., , ; Takahashi et al ., ); however, a complex network of multiple events makes it difficult to investigate the molecular mechanisms of individual cellular events. Our study offers a unique experimental system where the diploidization process can be easily tracked with labeled sexual gametes in the developing zygotes.…”

Section: Discussionmentioning

confidence: 99%

TALE homeobox heterodimer GSM1/GSP1 is a molecular switch that prevents unwarranted genetic recombination in Chlamydomonas

et al. 2019

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Eukaryotic sexual life cycles alternate between haploid and diploid stages, the transitions between which are delineated by cell fusion and meiotic division. Transcription factors in the TALE-class homeobox family, GSM1 and GSP1, predominantly control gene expression for the haploid-to-diploid transition during sexual reproduction in the unicellular green alga, Chlamydomonas reinhardtii. To understand the roles that GSM1 and GSP1 play in zygote development, we used gsm1 and gsp1 mutants and examined fused gametes that normally undergo the multiple organellar fusions required for the genetic unity of the zygotes. In gsm1 and gsp1 zygotes, no fusion was observed for the nucleus and chloroplast. Surprisingly, mitochondria and endoplasmic reticulum, which undergo dynamic autologous fusion/fission, did not undergo heterologous fusions in gsm1 or gsp1 zygotes. Furthermore, the mutants failed to resorb their flagella, an event that normally renders the zygotes immotile. When gsm1 and gsp1 zygotes resumed the mitotic cycle, their two nuclei fused prior to mitosis, but neither chloroplastic nor mitochondrial fusion took place, suggesting that these fusions are specifically turned on by GSM1/GSP1. Taken together, this study shows that organellar restructuring during zygotic diploidization does not occur by default but is triggered by a combinatorial switch, the GSM1/GSP1 dyad. This switch may represent an ancient mechanism that evolved to restrict genetic recombination during sexual development.

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Section: Discussionmentioning

confidence: 99%

TALE homeobox heterodimer GSM1/GSP1 is a molecular switch that prevents unwarranted genetic recombination in Chlamydomonas

et al. 2019

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“…These genes are excluded from the EMB dataset because the protein functions are specific to fertilization, not embryo development. A third example (DMP9) is excluded because insertion mutants were not available and the phenotype was observed with RNAi alone (Takahashi et al, 2018). A different mechanism of paternal inheritance is observed in knockouts of SSP, which encodes a receptor-associated protein kinase.…”

Section: Diversity Of Emb Protein Functionsmentioning

confidence: 99%

Genome‐wide identification of EMBRYO‐DEFECTIVE (EMB) genes required for growth and development in Arabidopsis

Meinke

2019

New Phytologist

140

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Summary With the emergence of high‐throughput methods in plant biology, the importance of long‐term projects characterized by incremental advances involving multiple laboratories can sometimes be overlooked. Here, I highlight my 40‐year effort to isolate and characterize the most common class of mutants encountered in Arabidopsis (Arabidopsis thaliana): those defective in embryo development. I present an updated dataset of 510 EMBRYO‐DEFECTIVE (EMB) genes identified throughout the Arabidopsis community; include important details on 2200 emb mutants and 241 pigment‐defective embryo (pde) mutants analyzed in my laboratory; provide curated datasets with key features and publication links for each EMB gene identified; revisit past estimates of 500–1000 total EMB genes in Arabidopsis; document 83 double mutant combinations reported to disrupt embryo development; emphasize the importance of following established nomenclature guidelines and acknowledging allele history in research publications; and consider how best to extend community‐based curation and screening efforts to approach saturation for this diverse class of mutants in the future. Continued advances in identifying EMB genes and characterizing their loss‐of‐function mutant alleles are needed to understand genotype‐to‐phenotype relationships in Arabidopsis on a broad scale, and to document the contributions of large numbers of essential genes to plant growth and development.

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“…DUO1 is responsible for division of germ cell into two sperms cells via regulation of mitotic cyclin CYCB1 accumulation during G2 phase [61]. Among its targets, there was downregulated DMP9/DAU2 (At5g39650), which is responsible for double fertilization and its silencing leads to seed abortion [62]. Last but not least, three zinc finger transcription factors DAZ1 (At2g17180), DAZ2 (At4g35280), DAZ3 (At4g35700) or vacuolar aquaporin TIP5;1 (At3g35700) important for pollen tube growth were included.…”

Section: Flower Bud Transcriptome and Proteomementioning

confidence: 99%

The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana

Fíla

Klodová

Potěšil

et al. 2020

IJMS

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The nascent polypeptide-associated (NAC) complex was described in yeast as a heterodimer composed of two subunits, α and β, and was shown to bind to the nascent polypeptides newly emerging from the ribosomes. NAC function was widely described in yeast and several information are also available about its role in plants. The knock down of individual NAC subunit(s) led usually to a higher sensitivity to stress. In Arabidopsis thaliana genome, there are five genes encoding NACα subunit, and two genes encoding NACβ. Double homozygous mutant in both genes coding for NACβ was acquired, which showed a delayed development compared to the wild type, had abnormal number of flower organs, shorter siliques and greatly reduced seed set. Both NACβ genes were characterized in more detail—the phenotype of the double homozygous mutant was complemented by a functional NACβ copy. Then, both NACβ genes were localized to nuclei and cytoplasm and their promoters were active in many organs (leaves, cauline leaves, flowers, pollen grains, and siliques together with seeds). Since flowers were the most affected organs by nacβ mutation, the flower buds’ transcriptome was identified by RNA sequencing, and their proteome by gel-free approach. The differential expression analyses of transcriptomic and proteomic datasets suggest the involvement of NACβ subunits in stress responses, male gametophyte development, and photosynthesis.

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The male gamete membrane protein DMP9/DAU2 is required for double fertilization in flowering plants

Cited by 68 publications

References 31 publications

TALE homeobox heterodimer GSM1/GSP1 is a molecular switch that prevents unwarranted genetic recombination in Chlamydomonas

TALE homeobox heterodimer GSM1/GSP1 is a molecular switch that prevents unwarranted genetic recombination in Chlamydomonas

Genome‐wide identification of EMBRYO‐DEFECTIVE (EMB) genes required for growth and development in Arabidopsis

The Beta Subunit of Nascent Polypeptide Associated Complex Plays A Role in Flowers and Siliques Development of Arabidopsis thaliana

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