The malate sensing two-component system MaeKR is a non-canonical class of sensory complex for C4-dicarboxylates

Miguel-Romero, Laura; Casino, Patricia; Landete, José María; Monedero, Vicente; Zúñiga, Manuel; Marina, Alberto

doi:10.1038/s41598-017-02900-z

Cited by 6 publications

(7 citation statements)

References 67 publications

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“…3b). The presence of sulfate ions at the active center of RRs lacking the phosphorylatable Asp has been previously reported for MaeR triggering its phosphorylated conformation 25 . Indeed, comparing the structure of RR468 D53A with RR468 bound to BeF 3 − (PDB 3GL9) 10 confirmed that the former presented the phosphorylated conformation ( Supplementary Fig.…”

Section: Hk853-rr468 Structures Show a Ph Independentsupporting

confidence: 54%

Revisiting the pH-gated conformational switch on the activities of HisKA-family histidine kinases

et al. 2020

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Histidine is a versatile residue playing key roles in enzyme catalysis thanks to the chemistry of its imidazole group that can serve as nucleophile, general acid or base depending on its protonation state. In bacteria, signal transduction relies on two-component systems (TCS) which comprise a sensor histidine kinase (HK) containing a phosphorylatable catalytic His with phosphotransfer and phosphatase activities over an effector response regulator. Recently, a pH-gated model has been postulated to regulate the phosphatase activity of HisKA HKs based on the pH-dependent rotamer switch of the phosphorylatable His. Here, we have revisited this model from a structural and functional perspective on HK853-RR468 and EnvZ-OmpR TCS, the prototypical HisKA HKs. We have found that the rotamer of His is not influenced by the environmental pH, ruling out a pH-gated model and confirming that the chemistry of the His is responsible for the decrease in the phosphatase activity at acidic pH.

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Section: Hk853-rr468 Structures Show a Ph Independentsupporting

confidence: 54%

Revisiting the pH-gated conformational switch on the activities of HisKA-family histidine kinases

et al. 2020

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“…IBR07 also contains the malolactic (MLE) and malate oxidative decarboxylase (ME) pathways, both involved in malate fermentation (Figure 3). MLE is responsible for the degradation of malate to lactate and it is encoded in a bicistronic operon regulated by the LysR‐family regulator MleR (Landete et al, 2013; Miguel‐Romero et al, 2017; Papadimitriou et al, 2016). The malolactic enzyme (MleS) is an NADP‐dependent enzyme involved in the decarboxylation of malate in a redox neutral process.…”

Section: Resultsmentioning

confidence: 99%

Genomic analysis revealed conserved acid tolerance mechanisms from native micro‐organisms in fermented feed

Terán

Mortera

Tubío

et al. 2021

J of Applied Microbiology

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Aims: Fermented feed is an agricultural practice used in many regions of the world to improve the growth performance of farm animals. This study aimed to identify and evaluate the lactic acid bacteria and yeast involved in the production of fermented feed. Methods and Results:We isolated and described two micro-organisms from autochthonous microbiota origin present in a regional feed product, Lactobacillus paracasei IBR07 (Lacticaseibacillus paracasei) and Kazachstania unispora IBR014 (Saccharomyces unisporum). Genome sequence analyses were performed to characterize both micro-organisms. Potential pathways involved in the acid response, tolerance and persistence were predicted in both genomes. Although L. paracasei and K. unispora are considered safe for animal feed, we analysed the presence of virulence factors, antibiotic resistance and pathogenicity islands. Furthermore, the Galleria mellonella model was used to support the safety of both isolates. Conclusions:We conclude that IBR07 and IBR014 strains are good candidates to be used as starter cultures for feed fermentation. Significance and Impact of the Study:The data presented here will be helpful to explore other biotechnological aspects and constitute a starting point for further studies to establish the consumption benefit of fermented feed in farm animal production.

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“…The genes of the GAS malic enzyme pathway are highly conserved and arranged as two diverging operons. MaeKR encodes the maeKR TCS, while maePE encodes a putative L-malate transporter (MaeP), and malic enzyme (MaeE) [ 88 ]. MaeKR is required for the expression of the maePE , in vitro .…”

Section: Discussionmentioning

confidence: 99%

In silico characterisation of the two-component system regulators of Streptococcus pyogenes

2018

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Bacteria respond to environmental changes through the co-ordinated regulation of gene expression, often mediated by two-component regulatory systems (TCS). Group A Streptococcus (GAS), a bacterium which infects multiple human body sites and causes multiple diseases, possesses up to 14 TCS. In this study we examined genetic variation in the coding sequences and non-coding DNA upstream of these TCS as a method for evaluating relationships between different GAS emm-types, and potential associations with GAS disease. Twelve of the 14 TCS were present in 90% of the genomes examined. The length of the intergenic regions (IGRs) upstream of TCS coding regions varied from 39 to 345 nucleotides, with an average nucleotide diversity of 0.0064. Overall, IGR allelic variation was generally conserved with an emm-type. Subsequent phylogenetic analysis of concatenated sequences based on all TCS IGR sequences grouped genomes of the same emm-type together. However grouping with emm-pattern and emm-cluster-types was much weaker, suggesting epidemiological and functional properties associated with the latter are not due to evolutionary relatedness of emm-types. All emm5, emm6 and most of the emm18 genomes, all historically considered rheumatogenic emm-types clustered together, suggesting a shared evolutionary history. However emm1, emm3 and several emm18 genomes did not cluster within this group. These latter emm18 isolates were epidemiologically distinct from other emm18 genomes in study, providing evidence for local variation. emm-types associated with invasive disease or nephritogenicity also did not cluster together. Considering the TCS coding sequences (cds), correlation with emm-type was weaker than for the IGRs, and no strong correlation with disease was observed. Deletion of the malate transporter, maeP, was identified that serves as a putative marker for the emm89.0 subtype, which has been implicated in invasive outbreaks. A recombination-related, subclade-forming DNA motif was identified in the putative receiver domain of the Spy1556 response regulator that correlated with throat-associated emm-pattern-type A-C strains.

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The malate sensing two-component system MaeKR is a non-canonical class of sensory complex for C4-dicarboxylates

Cited by 6 publications

References 67 publications

Revisiting the pH-gated conformational switch on the activities of HisKA-family histidine kinases

Revisiting the pH-gated conformational switch on the activities of HisKA-family histidine kinases

Genomic analysis revealed conserved acid tolerance mechanisms from native micro‐organisms in fermented feed

In silico characterisation of the two-component system regulators of Streptococcus pyogenes

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