The Making of a Competent Oocyte – A Review of Oocyte Development and Its Regulation

Tukur, Hammed A.; Aljumaah, Riyadh S.; Swelum, Ayman A.; Alowaimer, Abdullah N.; Saadeldin, Islam M.

doi:10.12750/jarb.35.1.2

Cited by 11 publications

(8 citation statements)

References 62 publications

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“…Using the in vitro bovine oocyte model, we sought to elucidate the interplay between these two biomolecules through studying the effect of NAM administration on the different steps of embryo development, in the presence and absence of melatonin. Oocyte maturation, the progression from GV stage to MII, is an essential step for successful fertilization and embryo development [39]. The distribution of actin was reported to influence oocyte maturation [40,41].…”

Section: Discussionmentioning

confidence: 99%

Melatonin Alleviates the Toxicity of High Nicotinamide Concentrations in Oocytes: Potential Interaction with Nicotinamide Methylation Signaling

Sheikh

Mesalam

Song

et al. 2021

Oxidative Medicine and Cellular Longevity

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Despite the numerous studies on melatonin and nicotinamide (NAM, the active form of vitamin B3), the linkage between these two biomolecules in the context of signaling pathways regulating preimplantation embryo development has not yet been investigated. In this study, we used bovine oocyte model to elucidate the effect of melatonin on the developmental competence of oocytes under the stress of high NAM concentrations. Results showed that NAM (20 mM) administration during in vitro maturation (IVM) significantly reduced oocyte maturation and actin distribution, while induced reactive oxygen species (ROS) accumulation and mitochondrial dysfunction, the multiple deleterious effects that were alleviated by melatonin (10−7 M). The RT-qPCR and/or immunofluorescence showed upregulation of the apoptosis (Caspase-3, Caspase-9, and BAX), autophagy (Beclin-1, LC3A, LC3B, ATG7, LAMP1, and LAMP2), cell cycle (P21, P27, and P53), and DNA damage (COX2 and 8-OxoG) specific markers in oocytes matured under NAM treatment, compared to NAM-melatonin dual-treated and the untreated ones. In addition, the total cleavage and blastocyst development rate, as well as the total number of cells and the inner cell mass (ICM) per blastocyst, were reduced, while DNA fragmentation was induced, in the group of NAM sole treatment than NAM-melatonin cotreatment and control. Inspecting the underlying mechanisms behind NAM-associated toxicity revealed an increase in transcription pattern of NAM methylation (NNMT and AHCY) genes in NAM-treated oocytes while the opposite profile was observed upon melatonin supplementation. In conclusion, to our knowledge, this is the first study reporting that melatonin can protect oocytes and embryos from NAM-induced injury through its ROS-scavenging activity together with potential interaction with NAM methylation signaling.

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Section: Discussionmentioning

confidence: 99%

Melatonin Alleviates the Toxicity of High Nicotinamide Concentrations in Oocytes: Potential Interaction with Nicotinamide Methylation Signaling

Sheikh

Mesalam

Song

et al. 2021

Oxidative Medicine and Cellular Longevity

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“…Esta evaluación fue realizada dentro de los 30 min luego de la aspiración folicular. Estos resultados son respaldados por estudios realizados en ovocitos de ratones, donde el ooplasma y la membrana nuclear inician a descomponerse recién alrededor de 29 min después de la aspiración de ovocitos del folículo ovárico (Otsuki y Nagai, 2007); sin embargo, en otros mamíferos tarda aún mayor tiempo, alrededor del 10 % de la reanudación de meiosis como vesícula germinal rota ocurre entre 90 a 115 min, posiblemente por tener insuficientes moléculas necesarias en el ciclo celular (Eppig et al, 1996); en este proceso varias moléculas están involucradas en secuencias complejas que conllevan a la maduración nuclear que es la progresión del ovocito desde la etapa de núcleo intacto (vesícula germinal) en detención de la profase I a la metafase II (Tukur et al, 2020).…”

Section: Discussionunclassified

“…Está evidenciado que los ovocitos inmaduros se detienen en el primer proceso meiótico, cuya reanudación inicia con la eliminación del contacto entre ovocito y su envoltura folicular, donde participan moléculas involucradas ya identificadas (Khajeh et al, 2017;Roelen, 2019). Este proceso requiere un determinado tiempo de lo contrario morfológicamente la vesícula germinal aún permanece intacta (Tukur et al, 2020). El sistema de comunicación celular a través de varios factores modula la detención y el reinicio de la meiosis de los ovocitos (Pioltine et al, 2020;Idrees et al, 2021).…”

Section: Discussionunclassified

“…La condición corporal con efecto del desequilibrio de nutrientes se asocia con el crecimiento de los folículos ováricos que pueden afectar la competencia de los ovocitos; parte de esta alteración parece ser el resultado de cambios moleculares que modifican el microambiente del ovocito en crecimiento y maduración (Bezdícek et al, 2020;Tukur et al, 2020). En general, la condición corporal depende de la nutrición y alimentación, las que tienen impacto sobre la competencia de los ovocitos, fertilidad y eficiencia reproductiva, es por dichas razones, las vacas con buena condición corporal tienen un buen rendimiento reproductivo (López-Gatius et al, 2003).…”

Section: Discussionunclassified

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Asociación entre calidad de ovocitos recuperados y condición corporal en vacas criollas

Tinco-Salcedo

Quispe-Gutiérrez

Zea-Gonzales

2021

Rev. Investig. Altoandin.

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Criollo high Andean cattle are of family economic importance for rural dwellers and contribute to food security. The objective of this study was to evaluate the quantity, quality, and nuclear status of ovarian oocytes according to the body condition of Criollo cows. Groups of cows were formed according to the body condition (1=emaciated, 5=obese): low (≤1.5), moderate (2 to 2.5) and high (≥3). Ovaries (n=212) were obtained from Criollo cows from the slaughterhouse. Oocytes were recovered by follicular aspiration classifying them into categories A, B, and C, placing a portion with ethanol-acetic acid (3:1) for 24 h, then stained with 2% Lacmoid. The nuclear status of the oocyte was evaluated, categorized as intact germinal vesicle (GV) and broken germinal vesicle (GVBD). The number of oocytes recovered per ovary among low, moderate, and high body condition cows were similar (p>0.05). There was an association between body condition and oocyte quality of category A (rs=0.49; P=0.001), B (rs=0.16; P=0.018); C (rs=-0.16; P=0.016). Higher percentages (p≤0.05) of oocytes of categories A and B were obtained in those of moderate and high body condition, as category C in low body condition. Body condition did not influence (p>0.05) oocyte nuclear status. No association was found (p=0.073) between body condition and oocytes with intact GV, nor with GVBD (p=0.737). It is concluded that body condition is associated with the quality of oocytes of category A, B and C, but not with the nuclear status of GV and GVBD in Criollo cows from the slaughterhouse.

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“…In mammals, oocytes remain arrested at the first prophase of meiosis in meiotic maturation ( Adhikari and Liu, 2014 ; Tukur et al, 2020 ). Abnormal spindle assembly connected to DNA damage likely occurs in oocytes during this extended period of arrest.…”

Section: Introductionmentioning

confidence: 99%

Melatonin Protects Against Mdivi-1-Induced Abnormal Spindle Assembly and Mitochondrial Superoxide Production During Porcine Oocyte Maturation

Yang

Joe

Bae

et al. 2021

Front. Cell Dev. Biol.

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Mitochondrial division inhibitor 1 (Mdivi-1) reportedly provides a close connection between oocyte maturation and mitochondrial function in pigs. N-acetyl-5-methoxy-tryptamine (melatonin) is known to be a representative antioxidant with the ability to rehabilitate meiotic maturation of porcine oocytes. However, the ability of melatonin to recover Mdivi-1-mediated disruption of spindle formation during meiotic maturation of porcine oocytes during in vitro maturation (IVM) has not been studied. Here, we first investigated changes in mitochondrial length, such as fragmentation and elongation form, in mature porcine oocytes during IVM. Mature oocytes require appropriate mitochondrial fission for porcine oocyte maturation. We identified a dose-dependent reduction in meiotic maturation in porcine oocytes following Mdivi-1 treatment (50, 75, and 100 μM). We also confirmed changes in mitochondrial fission protein levels [dynamin-related protein 1 phosphorylation at serine 616 (pDRP1-Ser616) and dynamin-related protein 1 (DRP1)], mitochondrial membrane potential, and ATP production in 75 μM Mdivi-1-treated oocytes. As expected, Mdivi-1 significantly reduced mitochondrial function and DRP1 protein levels and increased spindle abnormalities in porcine oocytes. In addition, we confirmed that melatonin restores abnormal spindle assembly and reduces meiotic maturation rates by Mdivi-1 during porcine oocyte maturation. Interestingly, the expression levels of genes that reduce DNA damage and improve tubulin formation were enhanced during porcine meiotic maturation. Taken together, these results suggest that melatonin has direct beneficial effects on meiotic maturation through tubulin formation factors during porcine oocyte maturation.

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The Making of a Competent Oocyte – A Review of Oocyte Development and Its Regulation

Cited by 11 publications

References 62 publications

Melatonin Alleviates the Toxicity of High Nicotinamide Concentrations in Oocytes: Potential Interaction with Nicotinamide Methylation Signaling

Melatonin Alleviates the Toxicity of High Nicotinamide Concentrations in Oocytes: Potential Interaction with Nicotinamide Methylation Signaling

Asociación entre calidad de ovocitos recuperados y condición corporal en vacas criollas

Melatonin Protects Against Mdivi-1-Induced Abnormal Spindle Assembly and Mitochondrial Superoxide Production During Porcine Oocyte Maturation

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