The major protein in the midgut of teneral Glossina morsitans morsitans is a molecular chaperone from the endosymbiotic bacterium Wigglesworthia glossinidia

Haines, Lee R.; Haddow, Jody D.; Aksoy, Serap; Gooding, R. H.; Pearson, Terry W.

doi:10.1016/s0965-1748(02)00063-2

Cited by 19 publications

(18 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As noted previously in the tsetse/ Wigglesworthia [36,37], aphid/ Buchnera [38] and weevil/ Sodalis [39] symbioses, transcripts encoding GroEL/GroES (associated with ‘chaperone and folding catalysts’) are the most abundant in the bacteriome data (figure 1 a ; electronic supplementary material, table S5). Beyond chaperonins, almost 10% of Wigglesworthia reads matched to a non-coding transfer–messenger RNA (tmRNA, or ssrA ) and its unique binding protein ( smpB ) (electronic supplementary material, table S2), which are involved in the degradation of non-functional peptides [40].…”

Section: Resultssupporting

confidence: 52%

Unravelling the relationship between the tsetse fly and its obligate symbiont Wigglesworthia : transcriptomic and metabolomic landscapes reveal highly integrated physiological networks

et al. 2017

View full text Add to dashboard Cite

Insects with restricted diets rely on obligate microbes to fulfil nutritional requirements essential for biological function. Tsetse flies, vectors of African trypanosome parasites, feed exclusively on vertebrate blood and harbour the obligate endosymbiont Wigglesworthia glossinidia. Without Wigglesworthia, tsetse are unable to reproduce. These symbionts are sheltered within specialized cells (bacteriocytes) that form the midgut-associated bacteriome organ. To decipher the core functions of this symbiosis essential for tsetse's survival, we performed dual-RNA-seq analysis of the bacteriome, coupled with metabolomic analysis of bacteriome and haemolymph collected from normal and symbiont-cured (sterile) females. Bacteriocytes produce immune regulatory peptidoglycan recognition protein (pgrp-lb) that protects Wigglesworthia, and a multivitamin transporter (smvt) that can aid in nutrient dissemination. Wigglesworthia overexpress a molecular chaperone (GroEL) to augment their translational/transport machinery and biosynthesize an abundance of B vitamins (specifically B1-, B2-, B3- and B6-associated metabolites) to supplement the host's nutritionally deficient diet. The absence of Wigglesworthia's contributions disrupts multiple metabolic pathways impacting carbohydrate and amino acid metabolism. These disruptions affect the dependent downstream processes of nucleotide biosynthesis and metabolism and biosynthesis of S-adenosyl methionine (SAM), an essential cofactor. This holistic fundamental knowledge of the symbiotic dialogue highlights new biological targets for the development of innovative vector control methods.

show abstract

Section: Resultssupporting

confidence: 52%

Unravelling the relationship between the tsetse fly and its obligate symbiont Wigglesworthia : transcriptomic and metabolomic landscapes reveal highly integrated physiological networks

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Sodalis glossinidius , a secondary symbiont of tsetse flies ( Glossina ) [34], were isolated at the University of Alberta according to the protocol of Welburn and Maudlin [35], modified by changing the method used to maintain asepsis prior to initial culture [36]. In brief, S. glossinidius from tsetse hemolymph were initially grown on monolayers of Aedes albopictus cells and after 20 days in culture were adapted to axenic growth in serum-free medium [36].…”

Section: Methodsmentioning

confidence: 99%

“…In brief, S. glossinidius from tsetse hemolymph were initially grown on monolayers of Aedes albopictus cells and after 20 days in culture were adapted to axenic growth in serum-free medium [36]. The relatively slow growing bacteria were maintained by passaging every 5 days.…”

Section: Methodsmentioning

confidence: 99%

Killing of Trypanosomatid Parasites by a Modified Bovine Host Defense Peptide, BMAP-18

et al. 2009

View full text Add to dashboard Cite

BackgroundTropical diseases caused by parasites continue to cause socioeconomic devastation that reverberates worldwide. There is a growing need for new control measures for many of these diseases due to increasing drug resistance exhibited by the parasites and problems with drug toxicity. One new approach is to apply host defense peptides (HDP; formerly called antimicrobial peptides) to disease control, either to treat infected hosts, or to prevent disease transmission by interfering with parasites in their insect vectors. A potent anti-parasite effector is bovine myeloid antimicrobial peptide-27 (BMAP-27), a member of the cathelicidin family. Although BMAP-27 is a potent inhibitor of microbial growth, at higher concentrations it also exhibits cytotoxicity to mammalian cells. We tested the anti-parasite activity of BMAP-18, a truncated peptide that lacks the hydrophobic C-terminal sequence of the BMAP-27 parent molecule, an alteration that confers reduced toxicity to mammalian cells.Methodology/Principal FindingsBMAP-18 showed strong growth inhibitory activity against several species and life cycle stages of African trypanosomes, fish trypanosomes and Leishmania parasites in vitro. When compared to native BMAP-27, the truncated BMAP-18 peptide showed reduced cytotoxicity on a wide variety of mammalian and insect cells and on Sodalis glossindius, a bacterial symbiont of the tsetse vector. The fluorescent stain rhodamine 123 was used in immunofluorescence microscopy and flow cytometry experiments to show that BMAP-18 at low concentrations rapidly disrupted mitochondrial potential without obvious alteration of parasite plasma membranes, thus inducing death by apoptosis. Scanning electron microscopy revealed that higher concentrations of BMAP-18 induced membrane lesions in the parasites as early as 15 minutes after exposure, thus killing them by necrosis. In addition to direct killing of parasites, BMAP-18 was shown to inhibit LPS-induced secretion of tumour necrosis factor alpha (TNF-α), a cytokine that is associated with inflammation and cachexia (wasting) in sleeping sickness patients. As a prelude to in vivo applications, high affinity antibodies to BMAP-18 were produced in rabbits and used in immuno-mass spectrometry assays to detect the intact peptide in human blood and plasma.Conclusions/SignificanceBMAP-18, a truncated form of the potent antimicrobial BMAP-27, showed low toxicity to mammalian cells, insect cells and the tsetse bacterial symbiont Sodalis glossinidius while retaining an ability to kill a variety of species and life cycle stages of pathogenic kinetoplastid parasites in vitro. BMAP-18 also inhibited secretion of TNF-α, an inflammatory cytokine that plays a role in the cachexia associated with African sleeping sickness. These findings support the idea that BMAP-18 should be explored as a candidate for therapy of economically important trypanosome-infected hosts, such as cattle, fish and humans, and for paratransgenic expression in Sodalis glossinidius, a bacterial symbiont in the tsetse vector, a...

show abstract

“…Thus, GroEL constitutes 10% of the total protein in Buchnera [29] and is also overproduced in other endosymbiotic bacteria of different insects [30][31][32][33] and Amoeba proteus [34].…”

Section: The High Functional Flexibility Of Groelmentioning

confidence: 99%

GroEL and the maintenance of bacterial endosymbiosis

2004

View full text Add to dashboard Cite

The major protein in the midgut of teneral Glossina morsitans morsitans is a molecular chaperone from the endosymbiotic bacterium Wigglesworthia glossinidia

Cited by 19 publications

References 47 publications

Unravelling the relationship between the tsetse fly and its obligate symbiont Wigglesworthia : transcriptomic and metabolomic landscapes reveal highly integrated physiological networks

Unravelling the relationship between the tsetse fly and its obligate symbiont Wigglesworthia : transcriptomic and metabolomic landscapes reveal highly integrated physiological networks

Killing of Trypanosomatid Parasites by a Modified Bovine Host Defense Peptide, BMAP-18

GroEL and the maintenance of bacterial endosymbiosis

Contact Info

Product

Resources

About