The Major Fraction of Deoxyribonuclease Activity from Human Urinary Proteins Purification and Properties

Abstract

The major fraction of deoxyribonuclease activity from human urinary protein was purified 40-fold in about 14% yield. The enzyme shows an isoelectric point at pH 4.2 and has a molecular weight of 33,600 ± 3,000. Optimum activity was shown at pH 6.8 in the presence of 12.5 mmol/1 Mg 2 * plus 1 mmol/1 Ca 2+ .