The Major Floral Promoter NtFT5 in Tobacco (Nicotiana tabacum) Is a Promising Target for Crop Improvement

Schmidt, Florentin J.; Zimmermann, Marius M; Wiedmann, David R.; Lichtenauer, Sophie; Grundmann, Lena; Muth, Jost; Twyman, Richard M.; Prüfer, Dirk; Noll, Gundula A.

doi:10.3389/fpls.2019.01666

“…Examining FT's immediate suspects in meristematic flowering processes did not reveal an expression pattern that is similar to FT mRNA (Table S1 and S2 ). Thirteen FT-like genes were identified in tobacco 34 ; out of these 13 genes, NtFT4 and NtFT5 were shown to function as florigen 35 , 36 . RNA–seq of leaf tissue from mature or juvenile leaves showed that NtFT4 is expressed in mature leaves and not in juvenile leaves, while NtFT5 is not expressed in the leaves.…”

Section: Discussionmentioning

confidence: 99%

“…We, therefore, conclude that there are tissue-specific florigen in tobacco and maybe other plants. Some florigen genes are expressed in the leaves, and some in the stem 35 .…”

Section: Discussionmentioning

confidence: 99%

Florigen governs shoot regeneration

Kutsher

¹

,

Fisler

²

,

Faigenboim

³

et al. 2021

Sci Rep

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It is widely known that during the reproductive stage (flowering), plants do not root well. Most protocols of shoot regeneration in plants utilize juvenile tissue. Adding these two realities together encouraged us to study the role of florigen in shoot regeneration. Mature tobacco tissue that expresses the endogenous tobacco florigen mRNA regenerates poorly, while juvenile tissue that does not express the florigen regenerates shoots well. Inhibition of Nitric Oxide (NO) synthesis reduced shoot regeneration as well as promoted flowering and increased tobacco florigen level. In contrast, the addition of NO (by way of NO donor) to the tissue increased regeneration, delayed flowering, reduced tobacco florigen mRNA. Ectopic expression of florigen genes in tobacco or tomato decreased regeneration capacity significantly. Overexpression pear PcFT2 gene increased regeneration capacity. During regeneration, florigen mRNA was not changed. We conclude that florigen presence in mature tobacco leaves reduces roots and shoots regeneration and is the possible reason for the age-related decrease in regeneration capacity.

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“…We showed that NtFT4 is functional as a signaling protein in mammalian cells, and our comprehensive transcriptome analysis in MCF‐7 cells identified a number of potential NtFT4‐specific targets. Although NtFT4 belongs to the specialized FT‐like subclade that has only evolved in flowering plants (Karlgren et al, 2011), tobacco PEBPs can not only be used to modify floral transition and thereby improve yield in tobacco crops (Beinecke et al, 2018; Schmidt et al, 2020), but can also regulate the growth and survival pathways of human cells. The specific and precise nature of NtFT4 activity in human cells is particularly intriguing, and the ability of NtFT4 to regulate intercellular communication, the cell cycle, and their links to protein synthesis should allow for the further modification of this cell line.…”

Section: Discussionmentioning

confidence: 99%

The tobacco phosphatidylethanolamine‐binding protein NtFT4 simultaneously improves vitality, growth, and protein yield in human cells

Kronenberg

¹

,

Schrödter

²

,

Noll

³

et al. 2021

Biotech & Bioengineering

Self Cite

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The production of biopharmaceutical proteins in mammalian cells by transient expression or stable transformation requires robust and viable cells. Cell line engineering must therefore balance improved cell growth and viability with high productivity. We tested the ability of nonmammalian phosphatidylethanolamine‐binding proteins to enhance cell proliferation in monolayers and suspension cultures. The tobacco protein NtFT4 improved the proliferation of multiple human cell lines. Viable cell density is usually impaired by efficient transfection, but we found that the number of HEK‐293TNtFT4 cells at the peak of protein expression was twice that of standard HEK‐293T cells, and the antibody yield increased by approximately one‐third. Improved growth and viability were observed in different cell lines, in different culture media, and also after transient transfection, suggesting the beneficial trait is consistent and transferable. Additional modifications could boost the productivity of high‐density HEK‐293TNtFT4 cells even further as we showed for a fluorescent marker protein and recombinant antibody expressed in monolayer cultures. The HEK‐293TNtFT4 cell line provides a new human model platform that increases cell proliferation, also achieving a fundamental improvement in recombinant protein expression.

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“…Examining FT's immediate suspects in meristematic owering processes did not reveal an expression pattern that is similar to FT mRNA (Table S1 and S2). Thirteen FT-like genes were identi ed in tobacco 34 ; out of these 13 genes, NtFT4 and NtFT5 were shown to function as origen 35,36 . RNA-seq of leaf tissue from mature or juvenile leaves showed that NtFT4 is expressed in mature leaves and not in juvenile leaves, while NtFT5 is not expressed in the leaves.…”

Section: Discussionmentioning

confidence: 99%

Florigen Governs Shoot Regeneration

Kutsher

¹

,

Fisler

²

,

Doron‐Faigenboim

³

et al. 2021

Preprint

0

View full text Add to dashboard Cite

It is widely known that during the reproductive stage (flowering), plants do not root well. Most protocols of shoot regeneration in plants utilize juvenile tissue. Adding these two realities together encouraged us to study the role of florigen in shoot regeneration. Mature tobacco tissue that expresses the endogenous tobacco florigen mRNA regenerates poorly, while juvenile tissue that does not express the florigen regenerates shoots well. Inhibition of Nitric Oxide (NO) synthesis reduced shoot regeneration as well as promoted flowering and increased tobacco florigen level. In contrast, the addition of NO (by way of NO donor) to the tissue increased regeneration, delayed flowering, reduced tobacco florigen mRNA. Ectopic expression of florigen genes in tobacco or tomato decreased regeneration capacity significantly. Overexpression pear PcFT2 gene increased regeneration capacity. During regeneration, florigen mRNA was not changed. We conclude that florigen presence in mature tobacco leaves reduces roots and shoots regeneration and is the possible reason for the age-related decrease in regeneration capacity.

show abstract

The Major Floral Promoter NtFT5 in Tobacco (Nicotiana tabacum) Is a Promising Target for Crop Improvement

Cited by 23 publications

References 65 publications

Florigen governs shoot regeneration

Florigen governs shoot regeneration

The tobacco phosphatidylethanolamine‐binding protein NtFT4 simultaneously improves vitality, growth, and protein yield in human cells

Florigen Governs Shoot Regeneration

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