The major endocannabinoid anandamide (AEA) induces apoptosis of human granulosa cells

Costa, Liliana M.; Moreia-Pinto, B.; Felgueira, Eduarda; Ribeiro, Ana S. F.; Rebelo, Irene; Fonseca, Bruno M.

doi:10.1016/j.plefa.2021.102311

Cited by 7 publications

(3 citation statements)

References 46 publications

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“…The present results showed that the anti-CB1R antibody revealed a band of 70 kDa, while the anti-CB2R antibody revealed a band of 55 kDa (Figure 1). The authors have recently demonstrated that anti-CB1R and anti-CB2R in the canine small intestine recognised slightly different bands; however, it should be emphasised that the present results in the canine synovial membrane were aligned with previous reports regarding the detection of CB1R (molecular weight between 35 and ~70 kDa) and CB2R (molecular weight of ~35 and ~60 kDa) using Western blot analysis [43][44][45][46]. The anti-GPR55 antibody recognised a major band around 35 kDa and its dimer at 70 kDa, as previously described [4,39], in the canine gastrointestinal tract (Figure 1, right panel).…”

Section: Western Blot Analysissupporting

confidence: 90%

Endocannabinoid System Receptors at the Hip and Stifle Joints of Middle-Aged Dogs: A Novel Target for the Therapeutic Use of Cannabis sativa Extract in Canine Arthropathies

Zamith Cunha,

Salamanca,

Mille

et al. 2023

Animals

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The endocannabinoid system (ECS) has emerged as a potential therapeutic target in veterinary medicine due to its involvement in a wide range of physiological processes including pain, inflammation, immune function, and neurological function. Modulation of the ECS receptors has been shown to have anti-inflammatory, analgesic, and immunomodulatory effects in various animal models of disease, including dogs with osteoarthritis. The goal of this study was to identify and compare the cellular expression and distribution of cannabinoid receptor type 1 (CB1R) and type 2 (CB2R) and the cannabinoid-related G protein-coupled receptor 55 (GPR55) on the synovial cells of hip and stifle joints of seven dogs of different breeds without overt signs of osteoarthritis (OA). The synovial membranes of seven hips and seven stifle joints were harvested post mortem. The expression of the CB1R, CB2R, and GPR55 present in the synovial tissues was investigated using qualitative and quantitative immunofluorescence and Western blot (Wb) analysis. Synoviocytes of the stifle and hip joints expressed CB1R, CB2R, and GPR55 immunoreactivity (IR); no significant differences were observed for each different joint. Cannabinoid receptor 2- and GPR55-IR were also expressed by macrophages, neutrophils, and vascular cells. The ECS receptors were widely expressed by the synovial elements of dogs without overt signs of OA. It suggests that the ECS could be a target for the therapeutic use of Cannabis sativa extract in canine arthropathies.

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Section: Western Blot Analysissupporting

confidence: 90%

Endocannabinoid System Receptors at the Hip and Stifle Joints of Middle-Aged Dogs: A Novel Target for the Therapeutic Use of Cannabis sativa Extract in Canine Arthropathies

Zamith Cunha,

Salamanca,

Mille

et al. 2023

Animals

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“…Cannabinoids were also described in human granulosa cells (hGCs) and the COV434 granulosa cell line. AEA treatment in hGCs and in the COV434 cell line decreased cell viability and induced apoptosis, causing an increase in caspase 2/7 activities, and in hGCs, it also increased caspase 8 activity (Costa et al 2021).…”

Section: Cannabinoid In Early Development: Pregancymentioning

confidence: 92%

The Role of Cannabinoids in CNS Development: Focus on Proliferation and Cell Death

Bockmann

Brito²,

Fragel-Madeira³

et al. 2022

Cell Mol Neurobiol

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The active principles of Cannabis sativa are potential treatments for several diseases, such as pain, seizures and anorexia. With the increase in the use of cannabis for medicinal purposes, a more careful assessment of the possible impacts on embryonic development becomes necessary. Surveys indicate that approximately 3.9% of pregnant women use cannabis in a recreational and/or medicinal manner. However, although the literature has already described the presence of endocannabinoid system components since the early stages of CNS development, many of their physiological effects during this stage have not yet been established. Moreover, it is still uncertain how the endocannabinoid system can be altered in terms of cell proliferation and cell fate, neural migration, neural differentiation, synaptogenesis and particularly cell death. In relation to cell death in the CNS, knowledge about the effects of cannabinoids is scarce. Thus, the present work aims to review the role of the endocannabinoid system in different aspects of CNS development and discuss possible side effects or even opportunities for treating some conditions in the development of this tissue.

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“…In addition, eCBs have been implicated in many behavioral processes, including memory [ 77 , 78 ], emotional state [ 79 , 80 ], feeding [ 81 ], inflammation [ 82 , 83 ], hemodynamic response [ 84 , 85 ], energy metabolism [ 86 , 87 ], pregnancy [ 88 , 89 ], and nociception [ 90 , 91 , 92 ]. Moreover, they also modulate the proliferation, motility, adhesion, and apoptosis of cells [ 93 , 94 ].…”

Section: Cannabinoids and Therapeutic Potential In Pain Reliefmentioning

confidence: 99%

Cannabinoid Analgesia in Postoperative Pain Management: From Molecular Mechanisms to Clinical Reality

Carrascosa,

Navarrete,

Saldaña

et al. 2024

IJMS

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Postoperative pain (POP) is a challenging clinical phenomenon that affects the majority of surgical patients and demands effective management to mitigate adverse outcomes such as persistent pain. The primary goal of POP management is to alleviate suffering and facilitate a seamless return to normal function for the patient. Despite compelling evidence of its drawbacks, opioid analgesia remains the basis of POP treatment. Novel therapeutic approaches rely on multimodal analgesia, integrating different pharmacological strategies to optimize efficacy while minimizing adverse effects. The recognition of the imperative role of the endocannabinoid system in pain regulation has prompted the investigation of cannabinoid compounds as a new therapeutic avenue. Cannabinoids may serve as adjuvants, enhancing the analgesic effects of other drugs and potentially replacing or at least reducing the dependence on other long-term analgesics in pain management. This narrative review succinctly summarizes pertinent information on the molecular mechanisms, clinical therapeutic benefits, and considerations associated with the plausible use of various cannabinoid compounds in treating POP. According to the available evidence, cannabinoid compounds modulate specific molecular mechanisms intimately involved in POP. However, only two of the eleven clinical trials that evaluated the efficacy of different cannabinoid interventions showed positive results.

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The major endocannabinoid anandamide (AEA) induces apoptosis of human granulosa cells

Cited by 7 publications

References 46 publications

Endocannabinoid System Receptors at the Hip and Stifle Joints of Middle-Aged Dogs: A Novel Target for the Therapeutic Use of Cannabis sativa Extract in Canine Arthropathies

Endocannabinoid System Receptors at the Hip and Stifle Joints of Middle-Aged Dogs: A Novel Target for the Therapeutic Use of Cannabis sativa Extract in Canine Arthropathies

The Role of Cannabinoids in CNS Development: Focus on Proliferation and Cell Death

Cannabinoid Analgesia in Postoperative Pain Management: From Molecular Mechanisms to Clinical Reality

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