The maize brevis plant1 is a type II inositol polyphosphate 5-phosphatase

Chen, Yu; Cai, Qinan; Shen, Hao

doi:10.1007/s13562-017-0433-7

Cited by 1 publication

(1 citation statement)

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“…Agronomic traits studied in the T 4 transgenics revealed a significant variation in the without much detrimental effects. Plant heights which serve as a general indicator of plant vigor were found to be decreased and might be possibly due to change in intermodal cell length by the role of inositol polyphosphate phosphatases 49 . The phenotypic effect observed on the root growth precludes the possibility of an altered phosphate homeostasis in transgenic lines.…”

Section: Discussionmentioning

confidence: 99%

Seed targeted RNAi-mediated silencing of GmMIPS1 limits phytate accumulation and improves mineral bioavailability in soybean

Kumar

Krishnan

et al. 2019

Sci Rep

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Phytic acid (PA), the major phosphorus reserve in soybean seeds (60–80%), is a potent ion chelator, causing deficiencies that leads to malnutrition. Several forward and reverse genetics approaches have ever since been explored to reduce its phytate levels to improve the micronutrient and phosphorous availability. Transgenic technology has met with success by suppressing the expression of the PA biosynthesis-related genes in several crops for manipulating their phytate content. In our study, we targeted the disruption of the expression of myo- inositol-3-phosphate synthase ( MIPS1 ), the first and the rate limiting enzyme in PA biosynthesis in soybean seeds, by both antisense (AS) and RNAi approaches, using a seed specific promoter, vicilin . PCR and Southern analysis revealed stable integration of transgene in the advanced progenies. The transgenic seeds (T 4 ) of AS (MS14-28-12-29-3-5) and RNAi (MI51-32-22-1-13-6) soybean lines showed 38.75% and 41.34% reduction in phytate levels respectively, compared to non-transgenic (NT) controls without compromised growth and seed development. The electron microscopic examination also revealed reduced globoid crystals in the Protein storage vacoules (PSVs) of mature T 4 seeds compared to NT seed controls. A significant increase in the contents of Fe 2+ (15.4%, 21.7%), Zn 2+ (7.45%, 11.15%) and Ca 2+ (10.4%, 15.35%) were observed in MS14-28-12-29-3-5 and MI51-32-22-1-13-6 transgenic lines, respectively, compared to NT implicating improved mineral bioavailability. This study signifies proof-of-concept demonstration of seed-specific PA reduction and paves the path towards low phytate soybean through pathway engineering using the new and precise editing tools.

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Section: Discussionmentioning

confidence: 99%