THE MAIN TRENDS IN DYNAMICS OF SOIL MICROBIOM DURING A LONG-TERM FIELD EXPERIMENT AS INDICATED BY HIGH THROUGHPUT SEQUENCING THE 16S-rRNA GENE LIBRARIES

Dumova, V.A.; Pershina, Elizaveta; Merzlyakova, Ya.V.; Kruglov, Yu. V.; Andronov, Evgeny

doi:10.15389/agrobiology.2013.5.85eng

Cited by 2 publications

(7 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…В почве Длительного полевого опыта представители этого филума были выявлены только в трех вариантах: монокультура озимой ржи, «вечный пар» и «севооборот во времени». Полученные данные о том, что грибы-микоризообразователи обнаружены в почве «вечного пара», подтверждают, что в почве постоянно сохраняется пул микроорганизмов, который служит источником изменения микробного сообщества [8].…”

Section: результаты и их обсуждениеunclassified

“…Ковриго с соавт. (2016) [8,9], однако она касалась только прокариотической части микробиома почвы и не затрагивала грибную составляющую. Надо отметить, что в целом информация о разнообразии грибов в почвах сельскохозяйственного использования складывается в основном на основании данных классических исследований по культивированию, и только в последние годы стали широко использоваться методы, независимые от культивирования [7,27].…”

Section: Introductionunclassified

See 1 more Smart Citation

Evaluation of the long -term effects of the permanent cropping on soil microbial communities

Alsayed

2024

Izv. Timirâzevsk. s-h. akad.

View full text Add to dashboard Cite

The study of soil microbial communities under different agricultural crops (flax, clover, barley, potato, winter rye) grown in permanent cropping and crop rotation was carried out. The effect of permanent cropping and crop rotation on the taxonomic profile of the prokaryotic and fungal components of the soil microbiome was studied. The effect of permanent cropping and crop rotation on the metabolic intensity and stability of soil biota in the absence of mineral and organic fertilisers was assessed. The subject of the research was the long-term experience established in 1912 by Professor A.G. Doyarenko at the Russian State Agrarian University – Moscow Timiryazev Agricultural Academy, founded in 1912 by Professor A.G. Doyarenko. It was shown that the highest OUT (operational taxonomic units) values for fungi were found in soils under fallow and crop rotation. For prokaryotes, however, the crop rotation variant had the lowest number of OTUs. Ascomycetes were found to be the dominant fungal taxon in all samples studied. Prokaryotes were dominated by Proteobacteria and Acidobacteriota, followed by Actinobacteriota and Firmicutes, and Chloroflexi were represented to a lesser extent. Among the archaea, the Crenarcheota phylum was dominant. It was shown that continuous cultivation of crops generally has a negative effect on the functioning of the microbial community. With an optimal metabolic coefficient of 0.2 in monoculture, they reached 0.6. The sustainability of the soil microbial community is reduced by the continuous cultivation of potatoes, flax and clover without organic and mineral fertilizers, as well as by the “perpetual fallow” option. Particularly unfavorable conditions were found in potato monoculture. Continuous cultivation of cereals, especially winter rye, does not lead to a significant decrease in the activity of soil biota and the stability of soil microbial communities. Crop rotation makes it possible to optimize microbiological processes in the soil and to increase the stability of the soil microbial community.

show abstract

Section: результаты и их обсуждениеunclassified

Section: Introductionunclassified

Evaluation of the long -term effects of the permanent cropping on soil microbial communities

Alsayed

2024

Izv. Timirâzevsk. s-h. akad.

View full text Add to dashboard Cite

show abstract

“…Внедрение в молекулярную экологию методов секвенирования нуклеотидных последовательностей позволило полнее исследовать почвенные микроорганизмы и изучить микробиологические особенности прокариотных сообществ почвы [6,7].…”

unclassified

“…Подтвердилась зональность таксономической структуры микробиомов: преобладание представителей филы Proteobacteria в почвах северных регионов и Actinobacteria -в южных. В почвах с низкими значениями рН отмечено увеличение численности бактерий филы Acidobacteria, а в щелочных почвах -филы Firmicutes [6,7].…”

unclassified

“…Полученные данные иллюстрируют зависимость распространения представителей различных бактериальных фил от экологических условий, из которых основное влияние на структуру почвенного сообщества микроорганизмов оказывают влажность и кислотнощелочной баланс почвы [6,7,10,11].…”

unclassified

See 1 more Smart Citation

Influence of Fresh Biological Chicken Droppings on the Microbiological Indicators of Low-Productive Soddy Podzolic Soil

Shchukin¹,

Pukhova

Sobolev³

et al. 2023

Innov. prodovol. bezop.

View full text Add to dashboard Cite

The article presents fresh biological dropping of caged chickens in crop production. After a thorough study of its environmental friendliness to the environment, this new biological dropping will reduce the cost of poultry farms for the disposal of dropping and use for more efficient cultivation of crops on marginal lands. The experiment used a high dose of organic matter - 120 t/ha, applied by spreading (control - without fertilisers). Sequencing of the metagenomes of soil samples showed that the soil microbiome’s phylogenetic composition was preserved due to the introduction of bacterial-fermented probiotic dropping.The authors also note that its taxonomic structure and functionality changed due to increased bacteria (with the different activity of taxa). Thus, the authors report an increase in the abundance of bacteria in the 1st sampling period from 6.9•107 to 1.3•108 copies/g of the soil of the 16S rRNA gene of bacteria in the plough horizon, and after 30 days - from 1.6•107 to 1.3×108 copies/g. For these purposes, opportunistic and pathogenic microorganisms in the fertilised soil found in all soil samples were also studied. A higher proportion of enterobacteria was noted in the control sample (0.6%), and with dropping, it did not exceed 0.15%. The content of pathogenic bacteria on a fertilised background in the 1st period of soil sampling practically did not change (0.7–0.8%), but in the 2nd period, it decreased sharply (from 2.5 to 1.1%). However, the authors note a decrease in the content of pathogenic bacteria. This decrease was accompanied by an increase in their abundance (from 3.9•105 to 1.4•106 copies/g), mainly Pseudomonas sp bacteria (from 2.1•105 to 9.3•105 copies/g), which is a consequence of a general increase in the abundance of bacteria in the microbiome against the background of enrichment of the soil with organic matter and an increase in soil fertility. The authors did not reveal a significant negative microbiological effect after the action of fresh biological chicken dropping on the soil’s ecological state at the specified research stage.

show abstract

THE MAIN TRENDS IN DYNAMICS OF SOIL MICROBIOM DURING A LONG-TERM FIELD EXPERIMENT AS INDICATED BY HIGH THROUGHPUT SEQUENCING THE 16S-rRNA GENE LIBRARIES

Cited by 2 publications

References 10 publications

Evaluation of the long -term effects of the permanent cropping on soil microbial communities

Evaluation of the long -term effects of the permanent cropping on soil microbial communities

Influence of Fresh Biological Chicken Droppings on the Microbiological Indicators of Low-Productive Soddy Podzolic Soil

Contact Info

Product

Resources

About