The Main Parameters of Cellular Immunity in Patients With Triple-Negative Breast Cancer: Relationship With Efficiency of Chemotherapy

Черткова, А. И.; Славина, Е. Г.; Шоуа, Э. К.; Жукова, Л. Г.; Окружнова, М А; Нуртдинова, В А; Борунова, А А; Dzhgamadze, N. Т.; Кадагидзе, З. Г.

doi:10.15789/1563-0625-2018-5-667-680

Cited by 3 publications

(1 citation statement)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Основными критериями оценки функциональной активности эффекторных лимфоцитов стали: внутриклеточная экспрессия перфорина (порообразующего белка цитолитических гранул) в CD8 + -и CD16 +клетках и показатель цитотоксического потенциала, вычисленный как доля перфорин-содержащих лимфоцитов в структуре CD8 + -и CD16 + -клеток. При онкологических заболеваниях часто встречается нормальное количество клеток эффекторов с выраженной функциональной анергией (уменьшением доли перфорин-позитивных клеток), что является неблагоприятным фактором [21,22].…”

Section: результатыunclassified

Subpopulation Structure of Peripheral Blood Lymphocytes of Donors

Борунова

Заботина

Шоуа

et al. 2020

Rossijskij bioterapevtičeskij žurnal

Self Cite

View full text Add to dashboard Cite

Introduction. Long-term monitoring of immune system parameters in cancer patient in FSBI “N.N. Blokhin Medical Research Center of Oncology” of the Ministry of Health of Russia, as well as the absence of an immunological research algorithm and a spectrum of significant markers, served as the basis for this study.Purpose. To present reference values and determine the normal range for subpopulations of systemic immunity lymphocytes.Materials and methods. The phenotype of peripheral blood lymphocytes was studied in 186 healthy donors (86 men and 100 women), mean age 41,9 ± 12,5 years. To assess the multivariate phenotype by flow cytometry, monoclonal antibodies to CD45, CD3, CD4, CD8, CD16, CD56, CD19, CD25, CD28, CD11b, CD127, HLA-Dr, TCR-γ / δ, Perforin and Granzime B labeled various fluorochromes.Results. A panel of markers of immunocompetent cells of systemic immunity was developed as a basis for assessing the state of the immune system of cancer patients. Reference values and normal boundaries are given for characterizing the linearity of cells with detailing of the phenotypic and functional heterogeneity of the population of CD8+-lymphocytes, activation markers and the pool of naive cells; characteristics of various types of regulatory cells, functional activity of cells of the effector link of immunity. A comparative analysis of immunoregulatory indices was carried out and the vulnerability of the formula CD3+CD4+/CD3+CD8+ to the CD4+/CD8+ index was proved.Conclusion. The spectrum of the proposed indicators is the product of many years of research carried out in the laboratory of clinical immunology of the Federal State Budgetary Institution “N.N. Blokhin National Medical Research Center of Oncology” оf the Ministry of Health of the Russian Federation, in order to search for significant criteria for assessing the state of the immune system in cancer.

show abstract

Section: результатыunclassified

Subpopulation Structure of Peripheral Blood Lymphocytes of Donors

Борунова

Заботина

Шоуа

et al. 2020

Rossijskij bioterapevtičeskij žurnal

Self Cite

View full text Add to dashboard Cite

show abstract

Age-Related Features of Systemic Antitumor Immune Response in Patients With Primary Operable Breast Cancer and Cancer of the Oral Mucosa

Черткова

Заботина

Циклаури

et al. 2020

Rossijskij bioterapevtičeskij žurnal

Self Cite

View full text Add to dashboard Cite

Introduction. Age is considered as an important clinical and pathological factor in cancer patients. Malignant tumors are more likely to develop in older people, but the disease is less aggressive than in young patients. According to various authors, the influence of age on the development of tumors largely depends on the age-related features of the immune system.The aim of the present study was to determine the relationship of indicators of systemic antitumor immune response with the age of patients with primary operable breast cancer and cancer of the oral mucosa.Materials and methods. The study included patients with all subtypes of primary-operable breast cancer (n = 145) and patients with cancer of the oral mucosa (n = 29). Immunophenotyping of peripheral blood lymphocytes was performed using a wide panel of monoclonal antibodies to markers of adaptive and innate immunity cells.Results. In elder patients (40 years and older) with primary-operable breast cancer, the percentage of activated CD25+ lymphocytes and CD4+CD25+ and CD3+CD4+ T cells, NKT cells, activated HLA-DR+ lymphocytes, including activated CD3+HLA-DR+ T cells before treatment, was statistically significantly higher than in patients younger than 40 years. Patients of this group showed increase of CD8+CD - 11b+CD28– CTLs and a decrease in the number of naive lymphocytes (CD4 – CD62L+ and CD8+CD11b – CD28+) in comparison with control percentage, and the downward trend in CD4+CD25+CD127– Treg, with increased numbers of CD4+CD25+ T cells. In patients with cancer of the oral mucosa, an increase in the number of cells of some populations of the immune effector link and a decrease in the number of suppressor lymphocytes were revealed with age.Conclusion. The results suggest that age-related differences in the state of systemic antitumor immune response contribute to a more favorable course of breast cancer and some other malignancies in older persons. It is obvious that the features of age differences in the immune response to the tumor should be taken into account when prescribing systemic therapy, including immunotherapy.All patients gave written informed consent to participate in the study

show abstract

ASSOCIATION OF NKT- AND ACTIVATED CD25<sup>+</sup> PERIPHERAL BLOOD LYMPHOCYTES WITH DISEASE FREE AND OVERALL SURVIVAL OF TRIPLE NEGATIVE BREAST CANCER PATIENTS

Черткова¹,

Славина²,

Заботина³

et al. 2020

Sib. onkol. ž.

Self Cite

View full text Add to dashboard Cite

Background. We previously found that a decrease in the number of NKT cells and activated CD 25+ peripheral blood lymphocytes (PBLs) before neoadjuvant chemotherapy was associated with an increased likelihood of disease progression in patients with locally advanced triple-negative breast cancer (TN BC).The purpose of this study was to determine the relationship between the initial number of NKT-and CD 25+ PBLs and relapsefree survival (RFS)/overall survival (OS ) in patients with TN BC who received neoadjuvant chemotherapy with cisplatin and paclitaxel followed by surgery.Material and Methods. The study included patients with stage II and III TN BC. The follow-up time was 36 and 66.9 months. Immediately before chemotherapy, the percentage of CD 3+CD 16+CD 56+ (NKT) -, CD 25+- and CD 8+ PBLs was determined by flow cytometry. Statistical analysis of the data was carried out using the Statistics 7 software package. The Kaplan-Meier method was used to determine the relationship between immunological parameters and RFS/ OS .Results. The decreased level of NKT cells before treatment was associated with a decrease in the 3-year RFS [Me: 20.1 (0.533 and 39.7) months] compared to that observed in patients with higher percentage of these cells than in the control (Me was not achieved). There were no statistically significant differences in the 3-year OS between the groups. The initially reduced number of CD 25+ lymphocytes in comparison with the control was associated with decreased rates of both RFS and OS . The difference in DFS and OS was more significant between the groups of patients who simultaneously had an increased initial number of both NKT and CD 25+ cells and patients in whom both cell populations were below normal levels.Conclusion. The initial (prior to chemotherapy) number of NKT and activated CD 25+ PBLs can apparently be a predictive factor in TN BC patients, who received neoadjuvant chemotherapy with cisplatin and paclitaxel.

show abstract

The Main Parameters of Cellular Immunity in Patients With Triple-Negative Breast Cancer: Relationship With Efficiency of Chemotherapy

Cited by 3 publications

References 26 publications

Subpopulation Structure of Peripheral Blood Lymphocytes of Donors

Subpopulation Structure of Peripheral Blood Lymphocytes of Donors

Age-Related Features of Systemic Antitumor Immune Response in Patients With Primary Operable Breast Cancer and Cancer of the Oral Mucosa

ASSOCIATION OF NKT- AND ACTIVATED CD25<sup>+</sup> PERIPHERAL BLOOD LYMPHOCYTES WITH DISEASE FREE AND OVERALL SURVIVAL OF TRIPLE NEGATIVE BREAST CANCER PATIENTS

Contact Info

Product

Resources

About