The main anthocyanin monomer of <i>Lycium ruthenicum</i> Murray induces apoptosis through the ROS/PTEN/PI3K/Akt/caspase 3 signaling pathway in prostate cancer DU-145 cells

Li, Zhanlong; Mi, Jia; Lu, Lu; Luo, Qing; Liu, Xi; Yan, Yamei; Jin, Bo; Cao, Yang; Zeng, Xiaoxiong; Ran, Linwu

doi:10.1039/d0fo02382e

Cited by 30 publications

(20 citation statements)

References 44 publications

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“…The main compound was identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The main anthocyanin compound from the fruits of L. ruthenicum was 3- O -[6- O -(4- O -(trans-p-coumaroyl)-a- l -rhamnopyranosyl)-b- d -glucopyranoside]-5- O -[b- d -glucopyranoside] ( Figure S1 ), which is consistent with previous results [ 13 , 14 ].…”

Section: Methodssupporting

confidence: 91%

“…It has been reported that the fruits of L. ruthenicum are rich in anthocyanins. 3- O -[6- O -(4- O -(trans-p-coumaroyl)-a- l -rhamnopyranosyl)-b- d -glucopyranoside]-5- O - [b- d -glucopyranoside] ( Figure S1 ) is considered to be the main component of anthocyanin from the fruits of L. ruthenicum [ 13 , 14 ]. It has been reported that anthocyanins from the fruits of L. ruthenicum (ACN) possess a wide range of biological functions, including antioxidant and anti-inflammatory properties and can change the gut microbiota and reduce the risk of chronic diseases, such as obesity [ 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

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Preventive Effects of Anthocyanins from Lyciumruthenicum Murray in High-Fat Diet-Induced Obese Mice Are Related to the Regulation of Intestinal Microbiota and Inhibition of Pancreatic Lipase Activity

Liu

Zhang

et al. 2022

Molecules

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Lyciumruthenicum Murray (L. ruthenicum) has been used both as traditional Chinese medicine and food. Recent studies indicated that anthocyanins are the most abundant bioactive compounds in the L. ruthenicum fruits. The purpose of this study was to investigate the preventive effects and the mechanism of the anthocycanins from the fruit of L. ruthenicum (ACN) in high-fat diet-induced obese mice. In total, 24 male C57BL/6J mice were divided into three groups: control group (fed a normal diet), high-fat diet group (fed a high-fat diet, HFD), and HFD +ACN group (fed a high-fat diet and drinking distilled water that contained 0.8% crude extract of ACN). The results showed that ACN could significantly reduce the body weight, inhibit lipid accumulation in liver and white adipose tissue, and lower the serum total cholesterol and low-density lipoprotein cholesterol levels compared to that of mice fed a high-fat diet. 16S rRNA gene sequencing of bacterial DNA demonstrated that ACN prevent obesity by enhancing the diversity of cecal bacterial communities, lowering the Firmicutes-to-Bacteroidota ratio, increasing the genera Akkermansia, and decreasing the genera Faecalibaculum. We also studied the inhibitory effect of ACN on pancreatic lipase. The results showed that ACN has a high affinity for pancreatic lipase and inhibits the activity of pancreatic lipase, with IC50 values of 1.80 (main compound anthocyanin) and 3.03 mg/mL (crude extract), in a competitive way. Furthermore, fluorescence spectroscopy studies showed that ACN can quench the intrinsic fluorescence of pancreatic lipase via a static mechanism. Taken together, these findings suggest that the anthocyanins from L. ruthenicum fruits could have preventive effects in high-fat-diet induced obese mice by regulating the intestinal microbiota and inhibiting the pancreatic lipase activity.

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Section: Methodssupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Preventive Effects of Anthocyanins from Lyciumruthenicum Murray in High-Fat Diet-Induced Obese Mice Are Related to the Regulation of Intestinal Microbiota and Inhibition of Pancreatic Lipase Activity

Liu

Zhang

et al. 2022

Molecules

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“…Moreover, the Akt inhibitor MK-2206 was reported to induce apoptosis in several different types of AML cells (MV-4-11, MOLM-13, OCI/AML3, and U-937). It is well accepted that some apoptotic death-related proteins are downstream of the PI3K/Akt pathway, such as caspase-3 and Bad (Nepstad et al 2020;Li et al 2021). Therefore, our data suggest that inhibition of PI3K/Akt may be involved in the induction of the anti-AML action of leonurine.…”

supporting

confidence: 52%

Anti-leukaemia effects of leonurine in vitro and in vivo

Zhuang

Ruan²,

Jin³

et al. 2021

gpb

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The present study was conducted to explore the anti-acute myeloid leukaemia (AML) effects of leonurine. HL-60 and U-937 cells were used to assess the antileukaemia effect of leonurine in vitro, and HL-60 and U-937 xenograft nude mice were used to evaluate its antitumour effect in vivo. Leonurine inhibited the proliferation of HL-60 and U-937 cells in a time-and dose-dependent manner. Moreover, leonurine therapy prevented the growth of tumours in both xenograft animal models. Leonurine could induce apoptosis in HL-60 and U-937 cells. The cytotoxic effects of leonurine on HL-60 and U-937 cells were associated with an increased ratio of Bax/Bcl-2, activation of caspase-3, caspase-8 and caspase-9, and increased expression of cytochrome c in the cytoplasm. Leonurine inhibited activation of the PI3K/Akt pathway in HL-60 and U-937 cells by lowering the phosphorylation levels of PI3K and Akt. Our results indicate that leonurine is a potential anti-AML agent, and this activity may be associated with its repression of the phosphorylation of PI3K and Akt.

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“…These researchers found that the anthocyanin monomer inhibits the proliferation of cell and induces apoptosis through the ROS/PTEN/PI3K/Akt/caspase 3 signaling pathway. 129 In a similar fashion, Pal et al revealed that delphinidin reduces cancer cell proliferation via inhibiting phosphorylation of Akt and MAPKs, and activation of PI2K. 130 On the other hand, Lim and Song demonstrated that delphinidin prevents the proliferation of ovarian cancer cells by deactivating ERK1/2 mitogen-activated protein kinase and PI3K/Akt signaling cascades.…”

Section: Discussion: Redox Regulation and Therapeutic Perspectives Of...mentioning

confidence: 96%

Redox Activity of Flavonoids: Impact on Human Health, Therapeutics, and Chemical Safety

Sarkar

Chaudhary

Jamaddar

et al. 2022

Chem. Res. Toxicol.

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The cost-effectiveness of presently used therapies is a problem in overall redox-based management, which is posing a significant financial burden on communities across the world. As a result, sophisticated treatment models that provide notions of predictive diagnoses followed by targeted preventive therapies adapted to individual patient profiles are gaining global acclaim as being beneficial to patients, the healthcare sector, and society as a whole. In this context, natural flavonoids were considered due to their multifaceted antioxidant, anti-inflammatory, and anticancer effects as well as their low toxicity and ease of availability. The aim of this review is to focus on the capacity of flavonoids to modulate the responsiveness of various diseases and ailments associated with redox toxicity. The review will also focus on the flavonoids' pathway-based redox activity and the advancement of redox-based therapies as well as flavonoids' antioxidant characteristics and their influence on human health, therapeutics, and chemical safety. Research findings indicated that flavonoids significantly exhibit various redox-based therapeutic responses against several diseases such as inflammatory, neurodegenerative, cardiovascular, and hepatic diseases and various types of cancer by activating the Nrf2/Keap1 transcription system, suppressing the nuclear factor κB (NF-κB)/IκB kinase inflammatory pathway, abrogating the function of the Hsp90/Hsf1 complex, inhibiting the PTEN/PI3K/Akt pathway, and preventing mitochondrial dysfunction. Some flavonoids, especially genistein, apigenin, amentoflavone, baicalein, quercetin, licochalcone A, and biochanin A, play a potential role in redox regulation. Conclusions of this review on the antioxidant aspects of flavonoids highlight the medicinal and folk values of these compounds against oxidative stress and various diseases and ailments. In short, treatment with flavonoids could be a novel therapeutic invention in clinical trials, as we hope.

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The main anthocyanin monomer of Lycium ruthenicum Murray induces apoptosis through the ROS/PTEN/PI3K/Akt/caspase 3 signaling pathway in prostate cancer DU-145 cells

Abstract: Pt3G inhibits DU-145 cell proliferation and induces apoptosis through the ROS/PTEN/PI3K/Akt/caspase-3 signaling pathway.

Cited by 30 publications

References 44 publications

Preventive Effects of Anthocyanins from Lyciumruthenicum Murray in High-Fat Diet-Induced Obese Mice Are Related to the Regulation of Intestinal Microbiota and Inhibition of Pancreatic Lipase Activity

Preventive Effects of Anthocyanins from Lyciumruthenicum Murray in High-Fat Diet-Induced Obese Mice Are Related to the Regulation of Intestinal Microbiota and Inhibition of Pancreatic Lipase Activity

Anti-leukaemia effects of leonurine in vitro and in vivo

Redox Activity of Flavonoids: Impact on Human Health, Therapeutics, and Chemical Safety

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