The mago nashi gene is required for the polarisation of the oocyte and the formation of perpendicular axes in Drosophila

Micklem, David; DasGupta, Ramanuj; Elliott, Heather; Gergely, Fanni; Davidson, Catherine M.; Brand, Andrea H.; González-Reyes, Acaimo; Johnston, Daniel St

doi:10.1016/s0960-9822(06)00218-1

Cited by 187 publications

(201 citation statements)

References 48 publications

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“…We also describe several GFP markers that allow the rearrangement and dynamic cellular extensions of the cells to be followed in detail for up to 4 hr. Analysis of a Tau-GFP line showed that the MT network in dissected egg chambers is not disturbed by the preparation, when compared with previous descriptions of Tau-GFP in halocarbon mounted egg chambers (Micklem et al, 1997).…”

Section: Discussionsupporting

confidence: 61%

“…To test whether the MT network is affected by our dissection and imaging methods, we analyzed a fly line expressing the MTbinding protein bovine Tau-GFP (Micklem et al, 1997). Tau is not expressed in the border cell cluster, as the expression of the GFP fusion is under the control of the maternal ␣4 tubulin promoter, which drives female germline-specific expression.…”

Section: Border Cell Migration Is Not Influenced By Fluorescent Imagimentioning

confidence: 99%

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Imaging the migrating border cell cluster in living Drosophila egg chambers

Tekotte

Tollervey

Davis

2007

Developmental Dynamics

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Cell migration is a key process in animal development and central to the spread of cancer. Border cell migration in Drosophila egg chambers is an excellent general model for cell migration, but lacks techniques for studying this process in living cells. Here, we describe a simple and effective method of preparing egg chambers in halocarbon oil. The movement and behavior of the migrating border cells can reproducibly be followed in up to 25 egg chambers simultaneously by time-lapse microscopy using a variety of green fluorescent protein markers on a widefield microscope over a period of 4 hr. Our studies reveal a remarkably linear migration route of the border cell cluster and highly dynamic activity within their cluster. Migrating cells rapidly alter their relative positions and generate transient protrusions. These activities are likely to play key roles in the mechanism of migration and cannot readily be analyzed using fixed samples. Developmental Dynamics 236:2818 -2824, 2007.

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Section: Discussionsupporting

confidence: 61%

Section: Border Cell Migration Is Not Influenced By Fluorescent Imagimentioning

confidence: 99%

Imaging the migrating border cell cluster in living Drosophila egg chambers

Tekotte

Tollervey

Davis

2007

Developmental Dynamics

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“…3B). For example, PL04006A1H09, is homologous to mago nashi, a gene required for germ-plasm assembly and axis determination in Drosophila (51)(52)(53). The ovaries in planarians are small, and because of their location in the animal and thickness of the specimen, the ability to detect these structures unambiguously by in situ hybridization typically requires high levels of expression.…”

Section: Generation Of New Molecular Markers For the Planarian Reprodmentioning

confidence: 99%

The planarian Schmidtea mediterranea as a model for epigenetic germ cell specification: Analysis of ESTs from the hermaphroditic strain

Zayas

Hernández

Habermann

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

140

177

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Freshwater planarians have prodigious regenerative abilities that enable them to form complete organisms from tiny body fragments. This plasticity is also exhibited by the planarian germ cell lineage. Unlike many model organisms in which germ cells are specified by localized determinants, planarian germ cells appear to be specified epigenetically, arising postembryonically from stem cells. The planarian Schmidtea mediterranea is well suited for investigating the mechanisms underlying epigenetic germ cell specification. Two strains of S. mediterranea exist: a hermaphroditic strain that reproduces sexually and an asexual strain that reproduces by means of transverse fission. To date, expressed sequence tags (ESTs) have been generated only from the asexual strain. To develop molecular reagents for studying epigenetic germ cell specification, we have sequenced 27,161 ESTs from two developmental stages of the hermaphroditic strain of S. mediterranea ; this collection of ESTs represents ≈10,000 unique transcripts. blast analysis of the assembled ESTs showed that 66% share similarity to sequences in public databases. We annotated the assembled ESTs using Gene Ontology terms as well as conserved protein domains and organized them in a relational database. To validate experimentally the Gene Ontology annotations, we used whole-mount in situ hybridization to examine the expression patterns of transcripts assigned to the biological process “reproduction.” Of the 53 genes in this category, 87% were expressed in the reproductive organs. In addition to its utility for studying germ cell development, this EST collection will be an important resource for annotating the planarian genome and studying this animal's amazing regenerative abilities.

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“…Possibilities include: (1) influences of exonic sequences adjoining the intron, which were preserved in our constructs and which can sometimes affect deposition of the exon junction complex (Le Hir et al, 2000, 2001b; (2) differences among introns in their associations with trans-factors, which can subsequently be transferred to the spliced mRNA to regulate downstream events Ephrussi, 2001, 2004;Le Hir et al, 2001a, 2003Lehmann and Nusslein-Volhard, 1986;Micklem et al, 1997;Mohr et al, 2001); and (3) effects of intron length and tertiary structure on splicing efficiency, which can also affect deposition of crucial trans-factors (Ohno et al, 2002). It seems, at least, that length alone cannot be the whole answer because, although the optimally functional nefm Intron II (2.9 kb) was notably longer than the non-functional nefm Intron I (0.9 kb) and mini spliceable Intron II (0.2 kb), the equally functional hbb2 Intron B (0.6 kb) was actually slightly shorter than nefm Intron I.…”

Section: Discussionmentioning

confidence: 99%

Post-transcriptional regulation mediated by specific neurofilament introns in vivo

Wang

Szaro

2016

Journal of Cell Science

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Neurons regulate genes post-transcriptionally to coordinate the supply of cytoskeletal proteins, such as the medium neurofilament (NEFM), with demand for structural materials in response to extracellular cues encountered by developing axons. By using a method for evaluating functionality of cis-regulatory gene elements in vivo through plasmid injection into Xenopus embryos, we discovered that splicing of a specific nefm intron was required for robust transgene expression, regardless of promoter or cell type. Transgenes utilizing the nefm 3′-UTR but substituting other nefm introns expressed little or no protein owing to defects in handling of the messenger (m)RNA as opposed to transcription or splicing. Post-transcriptional events at multiple steps, but mainly during nucleocytoplasmic export, contributed to these varied levels of protein expression. An intron of the β-globin gene was also able to promote expression in a manner identical to that of the nefm intron, implying a more general preference for certain introns in controlling nefm expression. These results expand our knowledge of intronmediated gene expression to encompass neurofilaments, indicating an additional layer of complexity in the control of a cytoskeletal gene needed for developing and maintaining healthy axons.

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The mago nashi gene is required for the polarisation of the oocyte and the formation of perpendicular axes in Drosophila

Cited by 187 publications

References 48 publications

Imaging the migrating border cell cluster in living Drosophila egg chambers

Imaging the migrating border cell cluster in living Drosophila egg chambers

The planarian Schmidtea mediterranea as a model for epigenetic germ cell specification: Analysis of ESTs from the hermaphroditic strain

Post-transcriptional regulation mediated by specific neurofilament introns in vivo

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