The MADS box gene AOM1 is expressed in reproductive meristems and flowers of the dioecious species Asparagus officinalis

Caporali, Elisabetta; Spada, Alberto; Losa, Alessia; Marziani, Giovanna

doi:10.1007/s004970000049

Cited by 23 publications

(32 citation statements)

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“…With the aim of understanding the possible involvement of MADS box genes in shoot apex transition and in sex differentiation, we are isolating MADS box genes involved in reproductive phenomena. In a previous work we isolated AOM1, a MADS box gene expressed in reproductive meristems and in male and female flowers (Caporali et al 2000). Here we describe the isolation and characterisation of two other asparagus MADS box genes, AOM3 and AOM4.…”

Section: Introductionmentioning

confidence: 93%

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AOM3 and AOM4 : two MADS box genes expressed in reproductive structures of Asparagus officinalis

Losa

Caporali

Spada

et al. 2004

Sexual Plant Reproduction

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The MADS box genes participate in different steps of vegetative and reproductive plant development, including the most important phases of the reproductive process. Here we describe the isolation and characterisation of two Asparagus officinalis MADS box genes, AOM3 and AOM4. The deduced AOM3 protein shows the highest degree of similarity with ZAG3 and ZAG5 of maize, OsMADS6 of rice and AGL6 of Arabidopsis thaliana. The deduced AOM4 protein shows the highest degree of similarity with AOM1 of asparagus, the SEP proteins of Arabidopsis and the rice proteins OsMADS8, OsMADS45 and OsMADS7. The high level of identity between AOM1 and AOM4 made impossible the preparation of probes specific for one single gene, so the hybridisation signal previously described for AOM1 is probably due to the expression of both genes. The expression profile of AOM3 and AOM1/AOM4 during flower development is identical, and similar to that of the SEP genes. Asparagus genes, however, are expressed not only in flower organs, but also in the different meristem present on the apical region of the shoot during the flowering season: the apical meristem and the three lateral meristems emerging from the leaf axillary region that will give rise to flowers and lateral inflorescences during flowering season, and to phylloclades and branches during the subsequent vegetative phase. The expression of AOM3 and AOM1/AOM4 in these meristems appears to be correlated with the reproductive function of the apex as the hybridisation signal disappears when the apex switches to vegetative function.

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Section: Introductionmentioning

confidence: 93%

“…The female-flower-specific cDNA library (Clontech, Palo Alto, Calif.) previously utilised for isolating the asparagus gene AOM1, was screened with the MADS box domain of this gene (Caporali et al 2000). Radioactive probes were prepared using a random primed labelling kit (Roche Molecular Biochemicals).…”

Section: Screening Of a Female-specific Cdna Librarymentioning

confidence: 99%

AOM3 and AOM4 : two MADS box genes expressed in reproductive structures of Asparagus officinalis

Losa

Caporali

Spada

et al. 2004

Sexual Plant Reproduction

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“…In the genus Asparagus, MADS-box genes correlating with the development of flower organs have been isolated and characterized: AGAMOUS-like genes (AVAG1 [12], AVAG2 [13]), DEFICIENS-like genes (AODEF [9] [14]), GLOBOSA-like genes (AOGLOA and AOGLOB [10]), and SEPALLATA-like genes (AOM1 [8], AOM3 and AOM4 [11], AOMADS1, AOMADS2 and AOMADS3 [15], and AVMADS1, AVMADS2 and AVMADS3 [15]). Ectopic expression experiments that employed floral homeotic mutants would provide information regarding the molecular dynamics that depend on these genes.…”

Section: Discussionmentioning

confidence: 99%

“…The ABC model became more complex, and now includes another class of genes referred to as the D-function genes [4] [5] and the E-function genes [6] [7]. The study of the molecular genetic mechanisms that control flower development in asparagus has advanced recently [8]- [16]. Toward a confirmation of the functional analyses of isolates, it was hoped that the transgenic asparagus plant could be brought out to early flowering for estimation of its morphological and histological aspects, since asparagus usually requires several years from seed to flower.…”

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Precocious <i>In-Vitro</i> Flowering of Perennial Asparagus (<i>Asparagus officinalis</i> L.) Regenerants with a Chemical Inducer

Komai¹,

Watanabe²,

Kanno³

et al. 2016

AJPS

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A precocious flowering system of regenerants in asparagus (Asparagus officinalis) was achieved by treatment with a chemical inducer. Somatic embryos withered completely by being processed for 8 -12 days with 200 μM n-propyl N-(3,4-dichlorophenyl)carbamate that had been dissolved in distilled water. In contrast, precocious flowering occurred at an extremely low rate (3.4%) when somatic embryos were processed in carbamate dissolved in Murashige and Skoog's liquid medium. To encapsulate the female and male embryos, we surveyed the optimum conditions of viscosity and concentration of sodium alginate for encapsulating the seeds, and we screened the values of 80 -120 cps and 2% -3%, respectively. The synthetic seeds produced also withered when they were processed with the carbamate dissolved in distilled water. However, when Murashige and Skoog's liquid medium was used for the solvent, the flowering frequency of the synthetic seeds was enhanced (13.3%). Based on our morphological and histological observations, female and male regenerants that were processed with the carbamate solution produced individual flower organs. The conversion of sex expression did not occur. A precocious flowering system would allow a significant reduction in the time required for perennial seedlings to flower and can, therefore, save time required for further experiments that employ floral homeotic mutants.

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“…Thus, studies in petaloid monocots should give a better idea of the molecular evolution of monocot flowers, as the homologies of the different whorls are unquestionable, even in the highly specialized orchid flower. Unfortunately, MADSbox gene expression has been studied in very few petaloid monocots (Caporali et al 2000;Kramer and Irish 2000;Tzeng and Yang 2001;Li et al 2002;Kanno et al 2003), including a few orchids (Lu et al 1993;Yu and Goh 2000;Hsu and Yang 2002;Johansen and Frederiksen 2002;Yu et al 2002). Orchids could be suitable objects for studying both general expression of the A-, B-, and C-class genes, and the SEPALLATA genes as well as for studying the influence of these genes on the development of specialized structures such as gynostemium, stigma, viscidium, and pollinium stalk (Johansen and Frederiksen 2002).…”

Section: Introductionmentioning

confidence: 99%

Molecular Basis of Development in Petaloid Monocot Flowers

Johansen¹,

Frederikson²

2006

aliso

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The molecular background of flower development has been intensively studied within core eudicots, and several studies have confirmed the extended ABC model as the molecular background of flower development in this plant group. The core eudicots are characterized as having one copy of each of the B-class genes and at least two copies of A-class genes: one is expressed in floral meristems, the other in inflorescence meristems. In monocots and non-core eudicots the validity of the ABC model is under discussion. Generally, more than one functional copy is found of at least one of the B-class genes. The A-class genes apparently are expressed in meristems of both flower and inflorescence. Morphologically petaloid stamens and styles are well known within the petaloid monocots, whereas the phenomenon is rare in core eudicots. A simple model based on the extra copies of B-class genes can explain the molecular background of petaloid stamens in the monocots; the only requirement is that two copies of the same gene have different expression patterns and are responsible for development of petals and stamens, respectively. The formation of petaloid styles can be explained in the same way, but this hypothesis requires that A-and C-class gene expression is not mutually exclusive in monocots. The difference in expression of the A-class genes outside the floral organs shows a fundamental difference between monocot and core eudicot flowers.

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The MADS box gene AOM1 is expressed in reproductive meristems and flowers of the dioecious species Asparagus officinalis

Cited by 23 publications

References 20 publications

AOM3 and AOM4 : two MADS box genes expressed in reproductive structures of Asparagus officinalis

AOM3 and AOM4 : two MADS box genes expressed in reproductive structures of Asparagus officinalis

Precocious <i>In-Vitro</i> Flowering of Perennial Asparagus (<i>Asparagus officinalis</i> L.) Regenerants with a Chemical Inducer

Molecular Basis of Development in Petaloid Monocot Flowers

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The MADS box gene AOM1 is expressed in reproductive meristems and flowers of the dioecious species Asparagus officinalis

Cited by 23 publications

References 20 publications

AOM3 and AOM4 : two MADS box genes expressed in reproductive structures of Asparagus officinalis

AOM3 and AOM4 : two MADS box genes expressed in reproductive structures of Asparagus officinalis

Precocious &lt;i&gt;In-Vitro&lt;/i&gt; Flowering of Perennial Asparagus (&lt;i&gt;Asparagus officinalis&lt;/i&gt; L.) Regenerants with a Chemical Inducer

Molecular Basis of Development in Petaloid Monocot Flowers

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Precocious <i>In-Vitro</i> Flowering of Perennial Asparagus (<i>Asparagus officinalis</i> L.) Regenerants with a Chemical Inducer