Within research on antagonistic personality traits, there is vigorous debate about a suitable measurement approach to Machiavellianism. A measure targeting the focal themes of the trait’s historical eponym divides Machiavellianism with respect to motivational aspects (Machiavellian Approach and Avoidance Questionnaire; Blötner & Bergold, 2022, https://doi.org/10.1037/pas0001069). Machiavellian Approach reflects strategic striving for advantages even at others’ expense and Machiavellian Avoidance encompasses misanthropically driven prevention of loss. Extant studies found this distinction to be suitable to model prototypically Machiavellian outcomes, such as deception and manipulation. Using two independent German samples (ntotal = 1,583; 63% self-identified as female), this research provided additional evidence concerning construct validity regarding assumed core features of the two facets. Among others, both facets were connected by low agreeableness, Machiavellian approach was especially characterized by high dominance seeking, and Machiavellian avoidance was associated with high mistrust. Furthermore, cross-cultural validity of the Machiavellian Approach and Avoidance Questionnaire across German (i.e., a prototypical WEIRD culture) and Serbian samples (i.e., an expectedly non-WEIRD culture; ntotal = 478 after propensity score matching for age and gender) was tested via differential item functioning. One item exhibited differential item functioning but its impact on scale and individual scores was negligible, suggesting cross-cultural validity of the Machiavellian Approach and Avoidance Questionnaire.