The lupeol content in Machaerium species by HPLC-APCI-MS/MS and the allelopathic action

Abstract

The use of extracts from plants and/or allelopathic compounds from natural products can minimize the use of pesticides in agriculture. This is a chemical study of Machaerium eriocarpum branch extract and the evaluation of the allelopathic potential of Machaerium hirtum (Vell.) Stellfed and M. eriocarpum Benth (Fabaceae) leaves and branches extracts. The triterpene lupeol was isolated from the hydroethanolic extract of M. eriocarpum branches. The quantification of this compound in all extracts was performed by HPLC-APCI-MS/MS and the highest amount of lupeol was observed in the leaves extract of M. eriocarpum. Allelopathy tests were carried out against seeds of Sorghum bicolor L. (sorghum), evaluating the final percentage of germination, germination speed index (GSI), root growth and number of lateral roots. The leaves extracts of both species were effective in the inhibition of the root growth as well as of the lateral roots. The branches extract of M. hirtum showed inhibitory effect in the root growth and lateral roots only in 50 and 100 mg.L -1 doses. The lupeol showed mainly a strong inhibition of seed germination, root growth and lateral root appearance at 100 μM. This study showed the potentiality of Machaerium extracts and lupeol as bioherbicides.

Exploring antifungal potential: The flavonoid composition of Machaerium villosum extracts against Cryptococcus neoformans

“…eriocarpum Benth, M. amplum Benth., and M. villosum Vogel (Yhiya et al, 2015;Tahira et al, 2021;Tahira, 2022). This last species is popularly known as "Jacarandá Paulista," and according to the literature, there is a study on the inhibitory action of the heartwood extract against Schistosoma mansoni in mice, an investigation of the genetic structure of the plant, reforestation of the Atlantic Forest, and evaluation of the nutrients in its composition (Garcia et al, 2010;Yhiya et al, 2015;Gilbert et al, 1970;Giudice-Neto et al, 2014;Almeida & Viani, 2020).…”

Estudo químico e fitotóxico do extrato hidroetanólico das folhas de >i<Machaerium amplum>/i< Benth

“…Os extratos de plantas contendo flavonoides em sua constituição podem apresentar as mais variadas atividades biológicas, como antioxidante, anticâncer, antiviral, anti-inflamatória, antibacteriana, antiparasitária, antifúngica, anti-hiperglicêmica, analgésica, anti-hipertensiva, hepatoprotetiva, anti-helmíntica e também a atividade alelopática (SALVADOR et al, 2009;KUMAR & PANDEY, 2013;KIM et al, 2017;MOUFFOUK et al, 2017;PAVANA et al, 2017;TIWARI & HUSAIN, 2017;BENTO et al, 2018;LIU et al, 2018;RAMMOHAN et al, 2019;BELOFSKY et al, 2020;MIN et al, 2020;SHRIVASTAV & SHRIVASTAV, 2020;SANNOMIYA et al, 2020;TAHERI et al, 2020;TAHIRA et al, 2021).…”

“…OLIVEIRA et al, 1968;OLLIS et al, 1968;BRAGA et al, 1968;HIGA et al, 2006;SANTOS et al, 2009 A Tabela 1 denota as potencialidades biológicas envolvendo os extratos e/ou substâncias isoladas de Machaerium, como também confirma o predomínio da ocorrência de flavonoides neste gênero. Dentre as diferentes atividades biológicas dos extratos e/ou substâncias isoladas deste gênero, vale ressaltar seu potencial fitotóxico (BENTO et al, 2018;TAHIRA et al, 2021). Um uso comum de A. salina é na alimentação de algumas espécies de peixes ornamentais, além de ser muito utilizado em experimentos laboratoriais (FARIAS, 2019).…”