The <i>in vitro </i>Specificity of Antilymphocyte Sera Produced by Either Cultured or Noncultured Lymphocytes

Membrane Antigens Characteristic of Human Lymphoid Cells in Established Cultures

“…Bernoco et al ( 1969) have characterbed a lymphocyte in vztro antigen using human bera. Rabbit antisera to whole cells ( Perper et al 1970) and purified membrane components 973, reviJrd, accepted 24 January 1974 (Mann et al 1971) have been employed to detect antigenic differences between fresh and cultured human lymphoid cells. T h e present report describes the preparation of rabbit antisera reacting with antigens specific for human lymphoid cell lines.…”

Lymphocyte Transformation Induced by Autologous Cells: Stimulation by Cultured Lymphoblast Lines

“…The existence of determiinants on lymphoblasts not found on lymphocytes has been suggested by cross-absorption studies usinlg antiserumii raised to lymiiphoblasts and to blood lvmphocytes (21). Recent studies by Mann, Rogentine, Halterman, and Leventhal (22) showed that a non-HL-A determinant found on cultured lymphoblasts is present on leukocytes from leukemic patients and some of their relatives but not on the leukocytes of unrelated normal individuals.…”

Cultured human lymphocytes

“…Several clinics are using heterologous ALG induced with cultured lymphocytes (Starzl, Najarian, Deodhar and Birtch, personal communications); other transplantation centers are developing similar programs. Perper et al (1970) produced antilymphocyte sera in rabbits with pooled fresh human lymphocytes and with a single human lymphoid cell line. The titer of cytotoxicity was approximately the same, but absorption studies revealed differences in specificity.…”