The &lt;em&gt;In ovo&lt;/em&gt; CAM-assay as a Xenograft Model for Sarcoma

Sys, Gwen; Lapeire, Lore; Stevens, Nikita; Favoreel, Herman; Forsyth, Ramses; Bracke, Marc; Wever, Olivier De

doi:10.3791/50522

Cited by 49 publications

(49 citation statements)

References 25 publications

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“…In previous studies, several osteosarcoma cell lines have been found to form solid tumors on CAMs, with a rich vascular response3637. In this study, to assess the anti-cancer activity of ELH in vivo , we examined the ability of HT1080 cells to form solid tumors on CAMs with or without ELH treatment.…”

Section: Resultsmentioning

confidence: 99%

Ethanol extract of Lophatheri Herba exhibits anti-cancer activity in human cancer cells by suppression of metastatic and angiogenic potential

Kim

et al. 2016

Sci Rep

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Lophatheri Herba (LH), dried leaf of Lophatherum gracile Brongn, has long been used to reduce thirst and treat fever and inflammation in Chinese medicine. Recent studies have shown that LH has anti-viral, anti-bacterial, anti-cancer, anti-oxidant, diuretic, and hyperglycemic properties. However, the effects of an ethanol extract of L. herba (ELH), at non-cytotoxic doses, on the metastatic and angiogenic abilities of malignant tumor cells have not been reported. We found that ELH significantly suppressed p38, JNK, and NF-κB activation and proteolytic activities under phorbol 12-myristate 13-acetate (PMA) stimulation, thus leading to a decrease in metastatic potential, including migration and invasion. In addition, ELH suppressed tumor-induced angiogenesis, including migration and tube formation in human umbilical vein endothelial cells (HUVECs) and microvessel sprouting from aortic rings via decreasing the pro-angiogenic factors in tumors. Interestingly, in ovo xenografts ELH-treated HT1080 cells did not increase in volume and eventually disappeared, owing to a lack of angiogenesis. Daily oral administration of ELH at 50 and 100 mg/kg markedly inhibited metastatic colonization of B16F10 cells in the lungs of C57BL/6J mice and caused no apparent side effects. These data collectively indicate that ELH is safe and may be useful for managing metastasis and growth of malignant cancers.

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Section: Resultsmentioning

confidence: 99%

Ethanol extract of Lophatheri Herba exhibits anti-cancer activity in human cancer cells by suppression of metastatic and angiogenic potential

Kim

et al. 2016

Sci Rep

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“…These include detailed observations of organ (e.g., eye, brain etc) or tissue development (e.g., blood vessel growth) or application of teratogenic agents. Manipulations include grafting of tissues onto the chorioallantoic membranes to study the effect of combining different tissue layers or studying tumor growth and metastasis 14 .…”

Section: Representative Resultsmentioning

confidence: 99%

Optimized <em>Ex-ovo</em> Culturing of Chick Embryos to Advanced Stages of Development

Cloney

Franz‐Odendaal

2015

JoVE

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Research in anatomy, embryology, and developmental biology has largely relied on the use of model organisms. In order to study development in live embryos model organisms, such as the chicken, are often used. The chicken is an excellent model organism due to its low cost and minimal maintenance, however they present observational challenges because they are enclosed in an opaque eggshell. In order to properly view the embryo as it develops, the shell must be windowed or removed. Both windowing and ex ovo techniques have been developed to assist researchers in the study of embryonic development. However, each of the methods has limitations and challenges. Here, we present a simple, optimized ex ovo culture technique for chicken embryos that enables the observation of embryonic development from stage HH 19 into late stages of development (HH 40), when many organs have developed. This technique is easy to adopt in both undergraduate classes and more advanced research laboratories where embryo manipulations are conducted. Video LinkThe video component of this article can be found at

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“…The mechanism of this transport seems to rely on the sequestration of calcium within endosome-like vesicles upon the initial uptake phase [94]. Interestingly, in vitro CAM ectodermal cells exhibit faster calcium influx rates when compared to human erythrocytes suggesting alternative calcium entry mechanisms [175].…”

Section: Cam As a Screening Platformmentioning

confidence: 99%

The chicken chorioallantoic membrane model in biology, medicine and bioengineering

2014

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The chicken chorioallantoic membrane (CAM) is a simple, highly vascularized extraembryonic membrane, which performs multiple functions during embryonic development, including but not restricted to gas exchange. Over the last two decades, interest in the CAM as a robust experimental platform to study blood vessels has been shared by specialists working in bioengineering, development, morphology, biochemistry, transplant biology, cancer research and drug development. The tissue composition and accessibility of the CAM for experimental manipulation, makes it an attractive preclinical in vivo model for drug screening and / or for studies of vascular growth. In this article we provide a detailed review of the use of the CAM to study vascular biology and response of blood vessels to a variety of agonists. We also present distinct cultivation protocols discussing their advantages and limitations and provide a summarized update on the use of the CAM in vascular imaging, drug delivery, pharmacokinetics and toxicology.

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The <em>In ovo</em> CAM-assay as a Xenograft Model for Sarcoma

Cited by 49 publications

References 25 publications

Ethanol extract of Lophatheri Herba exhibits anti-cancer activity in human cancer cells by suppression of metastatic and angiogenic potential

Ethanol extract of Lophatheri Herba exhibits anti-cancer activity in human cancer cells by suppression of metastatic and angiogenic potential

Optimized <em>Ex-ovo</em> Culturing of Chick Embryos to Advanced Stages of Development

The chicken chorioallantoic membrane model in biology, medicine and bioengineering

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