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2. An absorption band was noted in the red just above 600 millimicrons approximately at 620. No other absorption bands were present other than those for oxyhemoglobin.3. The same sample of blood which had been refrigerated in the meantime was reexamined twenty-four hours later. During the intervening time several portions of this blood had been removed for other types of tests. At this examination no absorption spectrum was noted.4. Six hours later (forty-eight hours after autopsy) the same sample was examined the third time. No absorption spectrum was present. At this time air was blown through a sample of the curling solution that had been used on the patient. This air and vaporized solution were led through a tube into a portion of the blood previously referred to. The aeration was carried out for something less than two minutes. This aerated blood was then examined spectroscopically, and a strongly visible absorption band was present between 610 and 620. 5. One of the two remaining original containers which had not been opened since the blood had been collected at autopsy was similarly examined spectroscopically. This blood showed the same absorption spectrum occurring between 610 and 620. The samples of blood from the patient contained little oxygen, as evidenced by the dark, almost purple color. This color could be changed to the bright red of oxyhemoglobin by shaking the undiluted blood in an open test tube for a few seconds.If normal blood is diluted with dilute hydrogen sulfide water, the absorption band at 610 to 620 does not appear until a drop of 10 per cent ammonium hydroxide is added. Once the band does appear, however, it remains despite being shaken in the presence of air and standing overnight. This corresponds to the usual behavior of sulfhemoglobin as given in the literature.It is felt that the disappearance of the band in the sample of the patient's blood was probably due to the lack of oxygénation previously described.From these data there is strong evidence that the same material present in the curling solution was also present in the patient's blood at the time of death. Furthermore, this substance appears to be of a volatile nature, since the absorption band disappeared when the blood was exposed to the open air.Examination of the curling solution used on the patient, labeled "Willat Wave DeLux Curling Solution, Heatless Permanent Wave Company, Distributors, San Francisco, Calif.," revealed the following :1. The solution was composed largely of ammonium sulfide (hydrogen sulfide and ammonia proved by laboratory test).Evaporation of a small portion gave a small residue which looked like elementary sulfur. This was not completely soluble in carbon disulfide and might have contained traces of other materials.2. A fresh bottle from the same shipment as that which was used on the patient gave a strong smell of hydrogen sulfide (by odor) as soon as the cap was removed.3. The curling solution was strongly alkaline to litmus. In fact, an alkaline action occurred on contact of the litmus with the f...
2. An absorption band was noted in the red just above 600 millimicrons approximately at 620. No other absorption bands were present other than those for oxyhemoglobin.3. The same sample of blood which had been refrigerated in the meantime was reexamined twenty-four hours later. During the intervening time several portions of this blood had been removed for other types of tests. At this examination no absorption spectrum was noted.4. Six hours later (forty-eight hours after autopsy) the same sample was examined the third time. No absorption spectrum was present. At this time air was blown through a sample of the curling solution that had been used on the patient. This air and vaporized solution were led through a tube into a portion of the blood previously referred to. The aeration was carried out for something less than two minutes. This aerated blood was then examined spectroscopically, and a strongly visible absorption band was present between 610 and 620. 5. One of the two remaining original containers which had not been opened since the blood had been collected at autopsy was similarly examined spectroscopically. This blood showed the same absorption spectrum occurring between 610 and 620. The samples of blood from the patient contained little oxygen, as evidenced by the dark, almost purple color. This color could be changed to the bright red of oxyhemoglobin by shaking the undiluted blood in an open test tube for a few seconds.If normal blood is diluted with dilute hydrogen sulfide water, the absorption band at 610 to 620 does not appear until a drop of 10 per cent ammonium hydroxide is added. Once the band does appear, however, it remains despite being shaken in the presence of air and standing overnight. This corresponds to the usual behavior of sulfhemoglobin as given in the literature.It is felt that the disappearance of the band in the sample of the patient's blood was probably due to the lack of oxygénation previously described.From these data there is strong evidence that the same material present in the curling solution was also present in the patient's blood at the time of death. Furthermore, this substance appears to be of a volatile nature, since the absorption band disappeared when the blood was exposed to the open air.Examination of the curling solution used on the patient, labeled "Willat Wave DeLux Curling Solution, Heatless Permanent Wave Company, Distributors, San Francisco, Calif.," revealed the following :1. The solution was composed largely of ammonium sulfide (hydrogen sulfide and ammonia proved by laboratory test).Evaporation of a small portion gave a small residue which looked like elementary sulfur. This was not completely soluble in carbon disulfide and might have contained traces of other materials.2. A fresh bottle from the same shipment as that which was used on the patient gave a strong smell of hydrogen sulfide (by odor) as soon as the cap was removed.3. The curling solution was strongly alkaline to litmus. In fact, an alkaline action occurred on contact of the litmus with the f...
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