The loss of Halloween gene function seriously affects the development and reproduction of Diaphorina citri (Hemiptera: Liviidae) and increases its susceptibility to pesticides

Zhang, Cong; Wan, Bin; Jin, Mengru; Wang, Jing; Xin, Tianrong; Zou, Zhiwen; Xia, Bing

doi:10.1016/j.pestbp.2023.105361

Cited by 5 publications

(4 citation statements)

References 58 publications

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“…In the supermodel insect, the fruit fly, Drosophila melanogaster, silencing Halloween genes led to a reduction in oviposition [28]. Similar results were also obtained in Diaphorina citri [29]. In the soft tick, Ornithodoros moubata, Spook expression was determined in the ovary from both final instar nymphs and adult females, and shade expression was frequently surged after engorgement and copulation in adult females, suggesting Spook and shade are involved in ovary development [30].…”

Section: Introductionsupporting

confidence: 64%

“…Therefore, we believe that our results demonstrated that the Halloween gene TuSpo is involved in the regulation of vitellogenin biosynthesis to govern the oviposition of the reproduction process in T. urticae. To a similar extent, in S. gregaria and D. citri, the expression levels of Halloween genes decreased after adult molting, then increased gradually during the female reproductive cycle [27,29,48]. In V. destructor, three Halloween genes, Spo, Dib, and Shd, were up-regulated during the reproductive stage [31].…”

Section: Discussionmentioning

confidence: 74%

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Ecdysteroid Biosynthesis Halloween Gene Spook Plays an Important Role in the Oviposition Process of Spider Mite, Tetranychus urticae

Wang,

Li,

et al. 2023

IJMS

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In insects, the ecdysteroid hormone regulates development and reproduction. However, its function in the reproduction process of spider mites is still unclear. In this study, we investigated the effect of the Halloween gene Spook on the oviposition of the reproduction process in a spider mite, Tetranychus urticae. The expression patterns of the ecdysteroid biosynthesis and signaling pathway genes, as analyzed by RT-qPCR, showed that the expression pattern of the Halloween genes was similar to the oviposition pattern of the female mite and the expression patterns of the vitellogenesis-related genes TuVg and TuVgR, suggesting that the Halloween genes are involved in the oviposition of spider mites. To investigate the function of the ecdysteroid hormone on the oviposition of the reproduction process, we carried out an RNAi assay against the Halloween gene Spook by injection in female mites. Effective silencing of TuSpo led to a significant reduction of oviposition. In summary, these results provide an initial study on the effect of Halloween genes on the reproduction in T. urticae and may be a foundation for a new strategy to control spider mites.

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Section: Introductionsupporting

confidence: 64%

Section: Discussionmentioning

confidence: 74%

Ecdysteroid Biosynthesis Halloween Gene Spook Plays an Important Role in the Oviposition Process of Spider Mite, Tetranychus urticae

Wang,

Li,

et al. 2023

IJMS

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“…Insect ecdysone, which regulates insect molting behavior, is crucial for growth in insects. Based on studies of the Halloween gene family, insect ecdysone can regulate reproduction [ 33 ]. The insect ecdysone that regulated molting behavior was found to be a sterol.…”

Section: Discussionmentioning

confidence: 99%

NPC Intracellular Cholesterol Transporter 1 Regulates Ovarian Maturation and Molting in Female Macrobrachium nipponense

Jiang,

Zhang,

Xiong

et al. 2024

IJMS

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NPC intracellular cholesterol transporter 1 (NPC1) plays an important role in sterol metabolism and transport processes and has been studied in many vertebrates and some insects, but rarely in crustaceans. In this study, we characterized NPC1 from Macrobrachium nipponense (Mn-NPC1) and evaluated its functions. Its total cDNA length was 4283 bp, encoding for 1344 amino acids. It contained three conserved domains typical of the NPC family (NPC1_N, SSD, and PTC). In contrast to its role in insects, Mn-NPC1 was mainly expressed in the adult female hepatopancreas, with moderate expression in the ovary and heart. No expression was found in the embryo (stages CS–ZS) and only weak expression in the larval stages from hatching to the post-larval stage (L1–PL15). Mn-NPC1 expression was positively correlated with ovarian maturation. In situ hybridization showed that it was mainly located in the cytoplasmic membrane and nucleus of oocytes. A 25-day RNA interference experiment was employed to illustrate the Mn-NPC1 function in ovary maturation. Experimental knockdown of Mn-NPC1 using dsRNA resulted in a marked reduction in the gonadosomatic index and ecdysone content of M. nipponense females. The experimental group showed a significant delay in ovarian maturation and a reduction in the frequency of molting. These results expand our understanding of NPC1 in crustaceans and of the regulatory mechanism of ovarian maturation in M. nipponense.

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“…In adult females of the locust Schistocerca gregaria , depletion of spo , phm (Marchal et al, 2011), dib , sad and shd (Schellens et al, 2022) expression lowers circulating ecdysteroid levels, which results in changes in the size and morphology of the eggs, reduction of number of oviposited eggs and number of emerging nymphs (Schellens et al, 2022). In the hemipteran Diaphorina citri , inhibition of sha and shd impairs ovarian development (Zhang et al, 2023). In general, knockdown of steroidogenic genes in adult females decreases the 20E pulse, which impairs ovarian development, and prevents oocyte maturation.…”

Section: Introductionmentioning

confidence: 99%

Dual function of the transcription factor Ftz‐f1 on oviposition in the cockroach Blattella germanica

Alborzi

Piulachs

2023

Insect Molecular Biology

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The transcription factor Ftz‐f1 has multiple functions in insect development in a spatial‐temporal line. One of these roles is in the insect ovaries, specifically in the regulation of steroidogenic enzymes production. We studied the function of F in Blattella germanica oogenesis, as it shows two moments of high expression in ovaries: before the imaginal moult, and just before ovulation in the adult. Injecting dsftz‐f1 into adult females, either just after the imaginal moult or just prior to choriogenesis, prevented oviposition, with differences between the two approaches. In 3‐day‐old adult females treated with dsftz‐f1 just after the emergence, the expression of ftz‐f1 was not modified, but the steroidogenic genes increased their expression. ftz‐f1 transcript levels in the ovaries of 5‐day‐old dsftz‐f1‐treated females were significantly depleted, and the expression levels of the same steroidogenic genes began to decrease. These results suggest that Ftz‐f1 regulates the expression of steroidogenic genes in B. germanica, with phm possibly being a key target. Ftz‐f1 has a different temporal function in the cytoskeleton of follicular cells of the basal ovarian follicles. Early in the gonadotrophic cycle, Ftz‐f1 promotes the expression of genes related to the cytoskeleton and muscle proteins, while at the end of the cycle it maintains the expression levels of these genes, thus ensuring correct ovulation.

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The loss of Halloween gene function seriously affects the development and reproduction of Diaphorina citri (Hemiptera: Liviidae) and increases its susceptibility to pesticides

Cited by 5 publications

References 58 publications

Ecdysteroid Biosynthesis Halloween Gene Spook Plays an Important Role in the Oviposition Process of Spider Mite, Tetranychus urticae

Ecdysteroid Biosynthesis Halloween Gene Spook Plays an Important Role in the Oviposition Process of Spider Mite, Tetranychus urticae

NPC Intracellular Cholesterol Transporter 1 Regulates Ovarian Maturation and Molting in Female Macrobrachium nipponense

Dual function of the transcription factor Ftz‐f1 on oviposition in the cockroach Blattella germanica

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