The longissimus thoracis muscle proteome in Alentejana bulls as affected by growth path

Almeida, André M.; Nanni, Paolo; Ferreira, Ana M.; Fortes, Claudia; Grossmann, Jonas; Bessa, Rui J.B.; Costa, P.

doi:10.1016/j.jprot.2016.10.020

Cited by 21 publications

(12 citation statements)

References 72 publications

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“…Our results were corroborated by those reported in the study of Connor et al 11 whereby genes relating to oxidative phosphorylation were not detected or down-regulated on day 14 of re-alimentation of CG compared to the control group. Similarly in the study of Alemida et al 30 several proteins related to energy metabolism had a higher abundance in the bulls on the continuous growth path compared to the bulls on the discontinuous growth trajectory. These results together may suggest an adaptation to reduce energy production from dietary derived nutrients, leading to less energy being produced for maintenance and therefore more energy available for growth processes.…”

Section: Discussionmentioning

confidence: 56%

“…As already mentioned, currently there has been only one previous proteomic study examining the effect of CG on the muscle proteome 30 . This study identified a total of 26 proteins in the longissimus thoracis muscle.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, a study from our own group by Keady et al (2013) has shown differential muscle proteome expression across bovine breeds varying in genetic merit for carcass weight 24 . However, to the knowledge of the authors, to date, there has been only one such proteomic assessment in relation to CG in cattle 30 . Almeida et al 30 examined the effects of continuous and discontinuous growth paths on protein abundance in Alentejana bulls using global proteomics.…”

mentioning

confidence: 99%

“…However, to the knowledge of the authors, to date, there has been only one such proteomic assessment in relation to CG in cattle 30 . Almeida et al 30 examined the effects of continuous and discontinuous growth paths on protein abundance in Alentejana bulls using global proteomics. In this study the bulls were allowed to undergo CG for 9 months, until they reached 24 months of age at which point the tissue was sampled.…”

mentioning

confidence: 99%

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Label-free quantitative proteomic analysis of M. longissimus dorsi from cattle during dietary restriction and subsequent compensatory growth

Mullins

Keogh

Kenny

et al. 2020

Sci Rep

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compensatory growth (cG) is a naturally occurring physiological process whereby an animal has the ability to undergo enhanced growth following a period of restricted feeding. this studies objective was to identify key proteins involved in the expression of cG. forty Holstein friesian bulls were equally assigned to one of four groups. R1 and R2 groups were subjected to restricted feed allowance for 125 days (Period 1). A1 and A2 animals had ad libitum access to feed in Period 1. Following Period 1, all animals from R1 and A1 were slaughtered. Remaining animals (R2 and A2) were slaughtered following ad libitum access to feed for successive 55 days (Period 2). M. longissimus dorsi samples were collected at slaughter from all animals. proteins were isolated from samples and subjected to label-free mass spectrometry proteomic quantification. Proteins which were differentially abundant during CG (n = 39) were involved in cellular binding processes, oxidative phosphorylation and mitochondrial function. there was also evidence for up regulation of three pathways involved in nucleotide biosynthesis. Genetic variants in or regulating genes pertaining to proteins identified in this study may hold potential for use as DNA based biomarkers for genomic selection of animals with a greater ability to undergo CG. Compensatory growth (CG) or catch up growth can be defined as a physiological process through which an animal's growth rate accelerates upon re-alimentation following a period of under nutrition, enabling an animal to reach its genetically predetermined growth potential 1. In beef production systems, feed accounts for up to 80% of the total direct costs incurred 2. Therefore, any method by which these costs can be reduced, without impacting on overall animal performance, would positively influence on farm profitability. The exploitation of the CG phenomenon is a common practice in beef production systems, but particularly in pastoral systems such as in Ireland and has proven useful in reducing over winter animal feed costs 3. This naturally occurring biological process is also utilised in worldwide beef production systems in 'stocker' or 'backgrounding' programs 4. Additionally during CG, animal's exhibit enhanced feed efficiency, making it a highly desired trait 5,6. Due to the usefulness of this trait a number of studies have investigated the physiological 5-10 , as well as the molecular control 11-18 of CG in cattle. Skeletal muscle has a great economic value accounting for up to 50% of the total body weight of an animal and is in the order of 25% of basal metabolic expenditure 19,20. This tissue also plays a major role in energy homeostasis. Therefore skeletal muscle (M.longissimus dorsi) was selected as the target tissue for the current study. Transcriptional profiles have shown that both dietary restriction and subsequent CG are associated with greater expression of genes and pathways involved in metabolism, cellular function, energy production and cellular reorganization 13-15. However, although transcriptomic dat...

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Section: Discussionmentioning

confidence: 56%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Label-free quantitative proteomic analysis of M. longissimus dorsi from cattle during dietary restriction and subsequent compensatory growth

Mullins

Keogh

Kenny

et al. 2020

Sci Rep

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“…Various modern animal management methods have been developed to improve growth efficiency and meat quality, including intensive fattening (10). However, when adequate nutrition is provided to yak by an intensive fattening system during the cold season, the efficiency of the production system is improved, and the final meat product is increased by satisfying the demand of the customer (11).…”

Section: Introductionmentioning

confidence: 99%

Effects of Intensive Fattening With Total Mixed Rations on Carcass Characteristics, Meat Quality, and Meat Chemical Composition of Yak and Mechanism Based on Serum and Transcriptomic Profiles

Liu

Xiong

et al. 2021

Front. Vet. Sci.

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The objective of this study was to investigate the effects of intensive fattening with total mixed rations (TMR) on carcass characteristics, meat quality, and chemical composition of the yak meat. Theoretical data has been provided for evaluating the quality of yak meat during natural grazing and short-term fattening. Based on the analysis, we found that in fattening yak, the carcass weight (CWT) was increased by 106.43%, whereas the cooking loss, tenderness, and drop loss were significantly improved due to higher intramuscular fat content and lower moisture (P < 0.05). Protein, fat, calcium, and amino acids were also much higher (P < 0.01) in fattening yak compared with the grazing yak. The levels of albumin (ALB), lactate dehydrogenase (LDH), triglyceride (TRIG), and amylase (AMYL) in serum indicated better nutritional status for fattening yaks. The transcriptomics analysis showed that the high expression of ACSL1 and ACACB genes improved the synthesis and deposition of fat in fattening yak, whereas the regulation of SLC7A8, ATP1A4, ATP1A1, SLC3A2, and CPA3 gene expression weakened the proteolysis. These results indicated that fattening with TMR improves the yield and quality of the yak meat.

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A novel dynamic proteomics approach for the measurement of broiler chicken protein fractional synthesis rate

Peinado-Izaguerri

Zarzuela

McLaughlin

et al. 2023

Rapid Comm Mass Spectrometry

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Rationale The study of protein synthesis in farm animals is uncommon despite its potential to increase knowledge about metabolism and discover new biomarkers of health and growth status. The present study describes a novel dynamic proteomics approach for the measurement of protein fractional synthesis rate (FSR) in broiler chickens. Methods Chickens received a 10 g/kg oral dose of 2H2O at day 21 of their life. Body water 2H abundance was measured in plasma samples using a portable Fourier transform infrared spectrometer. Free and protein‐bound amino acids (AAs) were isolated and had their 2H enrichment measured by gas chromatography with mass spectrometry (GC/MS). Peptide 2H enrichment was measured by proteomics analysis of plasma and muscle samples. Albumin, fibrinogen and muscle protein FSR were calculated from GC/MS and proteomics data. Results Ala appeared to be more enriched at the site of protein synthesis than in the AA free pools. Glu was found to be the AA closest to isotopic equilibrium between the different AA pools. Glu was used as an anchor to calculate n(AA) values necessary for chicken protein FSR calculation in dynamic proteomics studies. FSR values calculated using proteomics data and GC/MS data showed good agreement as evidenced by a Bland–Altman residual plot. Conclusions A new dynamic proteomics approach for the measurement of broiler chicken individual protein FSR based on the administration of a single 2H2O oral bolus has been developed and validated. The proposed approach could facilitate new immunological and nutritional studies on free‐living animals.

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The longissimus thoracis muscle proteome in Alentejana bulls as affected by growth path

Cited by 21 publications

References 72 publications

Label-free quantitative proteomic analysis of M. longissimus dorsi from cattle during dietary restriction and subsequent compensatory growth

Label-free quantitative proteomic analysis of M. longissimus dorsi from cattle during dietary restriction and subsequent compensatory growth

Effects of Intensive Fattening With Total Mixed Rations on Carcass Characteristics, Meat Quality, and Meat Chemical Composition of Yak and Mechanism Based on Serum and Transcriptomic Profiles

A novel dynamic proteomics approach for the measurement of broiler chicken protein fractional synthesis rate

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