The long-term protective effects of short-interval postconditioning in testicular ischemia-reperfusion injury in rats

Özkisacik, Sezen; Erdem, Ali Onur; Durmaz, Ozge; Çulhacı, Nil; Gürsoy, Harun; Yazıcı, Mesut

doi:10.1016/j.jpedsurg.2011.10.070

Cited by 15 publications

(13 citation statements)

References 21 publications

(23 reference statements)

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“…()) first demonstrated that IPostC specifically protects both testes after unilateral testicular ischaemia–reperfusion and, accordingly, Ozkisacik et al . () confirmed these observations.…”

Section: Introductionsupporting

confidence: 75%

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Effects of ischaemic post‐conditioning on the early and late testicular damage after experimental testis ischaemia–reperfusion

et al. 2013

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SUMMARYIschaemic post-conditioning (IPostC) might represent an innovative surgical approach to protect organs from ischaemia and reperfusion (I/R) injury. We investigated the molecular mechanisms underlying the contrasting effects of IPostC on the early and late damage induced by testicular I/R injury. Testis I/R was induced by occluding the right testicular vessels using a clip. Male rats were divided into the following groups: sham, I/R and I/R + IPostC. In the I/R group, the clip was removed after 60 min of ischaemia, and reperfusion was allowed for 30 min, 1 and 30 days. In the I/R + IPostC group, three cycles of 30-sec reperfusion and 30-sec ischaemia were performed after 60 min of ischaemia and then reperfusion followed up for 30 min, 1 and 30 days. Following 30-min reperfusion, there was an increase in mitogen-activated protein kinases (MAPKs) in I/R rats; after 1 day of reperfusion, interleukin-6, tumour necrosis factor-a and nuclear factor-jB (NF-jB) expression were significantly increased; IjB-a expression reduced; and a marked damage in both testes was observed. IPostC inhibited MAPKs, cytokines and NF-jB expression, augmented IjB-a expression and decreased histological damage in testes subjected to I/R. After 30 days of reperfusion, I/R injury activated the apoptosis machinery, caused severe histological damage and reduced spermatogenic activity. By contrast, IPostC did not modify the apoptotic markers, the histological alterations as well as spermatogenic activity following 30 days of reperfusion. Our data demonstrate that IPostC protects the testis from the early damage induced by I/R injury, but it does not protect against the late damage.

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Section: Introductionsupporting

confidence: 75%

“…However, a recent study by Ozkisacik et al . () reported beneficial effects on testis still 60 days after the surgery in both testes. However, the authors did not investigate the molecular mechanisms that may substantiate this effect.…”

Section: Discussionmentioning

confidence: 92%

Effects of ischaemic post‐conditioning on the early and late testicular damage after experimental testis ischaemia–reperfusion

et al. 2013

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“…The histologic parameters studied are listed in Table 1, as described by Cosentino and colleagues [11–13]. Each testis was scored from 0 to 4 for each parameter, with the higher numbers indicating a more pathologic condition.…”

Section: Methodsmentioning

confidence: 99%

“…1. Each histologic parameter was compared between the cold ischemia and warm ischemia groups and total mean histology score was compared between the two groups [13]. …”

Section: Methodsmentioning

confidence: 99%

Evaluation of cold ischemia for preservation of testicular function during partial orchiectomy in the rat model

McNamara

Madden‐Fuentes

Routh

et al. 2014

Journal of Pediatric Urology

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Objective We hypothesized that cold ischemia during partial orchiectomy would lead to higher serum testosterone levels and preservation of testicular architecture than warm ischemia in a prepubescent rat model. Materials and methods Eighteen prepubescent male Sprague–Dawley rats were randomized to three different surgical groups: sham surgery, bilateral partial orchiectomy with 30 min of cord compression with cold ischemia, or bilateral partial orchiectomy with 30 min of cord compression with warm ischemia. Animals were killed at puberty, and serum, sperm, and testicles were collected. Histological tissue injury was graded by standardized methodology. Results Mean serum testosterone levels were 1445 ± 590 pg/mL for the sham group, 449 ± 268 pg/mL for the cold ischemia group and 879 ± 631 pg/mL for the warm ischemia group (p = 0.12). Mean sperm counts were 2.1 × 107 for sham, 4.4 × 106 for cold ischemia, and 9.9 × 106 for the warm ischemia groups (p = 0.48). Histological evaluation revealed significant difference in tissue injury grading with more injury in the cold ischemia than in the warm ischemia group (p = 0.01). Conclusions In our preclinical rat model, we found no benefit for cold ischemia over warm ischemia at 30 min.

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“…Ischemic post-conditioning is an innovative surgical technique against reperfusion injury, defined as rapid intermittent interruptions of blood flow at the early phase of reperfusion. Ischemic post-conditioning mechanically alters hydrodynamics of reperfusion and has been demonstrated to have an early beneficial effect in tIR (72). However, its role against late damage in tIR remains controversial (73).…”

Section: Physical Therapiesmentioning

confidence: 99%

Medical perspective in testicular ischemia-reperfusion injury

Arena

Iacona

Antonuccio

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. Testicular torsion or torsion of the spermatic cord is one of the most serious urological conditions. It causes testicular injury, which potentially leads to male subfertility. The turning of the spermatic cord and spermatic structures around themselves results in biochemical and histological changes; however, following testicular detorsion, tissues undergo reperfusion that causes more severe damage than that induced by ischemia. Since the primary causes of testicular damage are reactive oxygen species production, an increase in intra-mitochondrial calcium concentration and an increased rate of cellular apoptosis, different medications may potentially be effective. It seems that several medications, experimentally and sometimes clinically, serve an adjuvant role in the cellular damage that occurs following ischemia-reperfusion. Antioxidants, calcium channel blockers, phytotherapeutical medicinals, anaesthetics, hormones and platelet inhibitors may potentially create a solid basis for an adjuvant restoring therapy and ameliorate testicular function following torsion. The current study aimed to review the relevant literature and discuss the actions of a number of molecules that may protect the testes during ischemia/reperfusion injury.

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The long-term protective effects of short-interval postconditioning in testicular ischemia-reperfusion injury in rats

Cited by 15 publications

References 21 publications

Effects of ischaemic post‐conditioning on the early and late testicular damage after experimental testis ischaemia–reperfusion

Effects of ischaemic post‐conditioning on the early and late testicular damage after experimental testis ischaemia–reperfusion

Evaluation of cold ischemia for preservation of testicular function during partial orchiectomy in the rat model

Medical perspective in testicular ischemia-reperfusion injury

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