The long-term effect of active immunization against inhibin in goats

Wang, X.L.; El-Gayar, M.; Knight, P. G.; Holtz, W.

doi:10.1016/j.theriogenology.2008.07.024

Cited by 18 publications

(8 citation statements)

References 25 publications

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“…The present findings also raise questions about the mode of action of inhibin αC subunit auto-immunization, a vaccination procedure that has been found to enhance ovarian follicle development and raise ovulation rate in several species including cattle 36,37 , sheep 38–40 and goat 41,42 . Previously, it was considered that this action primarily involved neutralization of inhibin negative feedback action on pituitary FSH secretion, resulting in raised FSH levels that, in turn, enhance follicle development.…”

Section: Discussionmentioning

confidence: 82%

‘Free’ inhibin α subunit is expressed by bovine ovarian theca cells and its knockdown suppresses androgen production

Laird

Glister

Cheewasopit

et al. 2019

Sci Rep

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Inhibins are ovarian dimeric glycoprotein hormones that suppress pituitary FSH production. They are synthesised by follicular granulosa cells as α plus βA/βB subunits (encoded by INHA, INHBA, INHBB, respectively). Inhibin concentrations are high in follicular fluid (FF) which is also abundant in ‘free’ α subunit, presumed to be of granulosal origin, but its role(s) remains obscure. Here, we report the unexpected finding that bovine theca cells show abundant INHA expression and ‘free’ inhibin α production. Thus, theca cells may contribute significantly to the inhibin α content of FF and peripheral blood. In vitro, knockdown of thecal INHA inhibited INSL3 and CYP17A1 expression and androgen production while INSL3 knockdown reduced INHA and inhibin α secretion. These findings suggest a positive role of thecal inhibin α on androgen production. However, exogenous inhibin α did not raise androgen production. We hypothesised that inhibin α may modulate the opposing effects of BMP and inhibin on androgen production. However, this was not supported experimentally. Furthermore, neither circulating nor intrafollicular androgen concentrations differed between control and inhibin α-immunized heifers, casting further doubt on thecal inhibin α subunit having a significant role in modulating androgen production. Role(s), if any, played by thecal inhibin α remain elusive.

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Section: Discussionmentioning

confidence: 82%

‘Free’ inhibin α subunit is expressed by bovine ovarian theca cells and its knockdown suppresses androgen production

Laird

Glister

Cheewasopit

et al. 2019

Sci Rep

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“…Besides, previous superovulation studies showed that immunization against inhibin, combined with the conventional superovulation protocol, significantly improved embryo quantity and quality following superovulation and insemination in herbivorous livestock (Holtz et al., 2012; Li et al., 2009, 2011; Wang et al., 2009). Concomitantly, increased concentrations of plasma P4 were observed when embryo recovery during the luteal phase (Li et al., 2011; Liu et al., 2013; Medan, Akagi, et al., 2004; Mei et al., 2009).…”

Section: Discussionmentioning

confidence: 99%

Improving ovulation in gilts using anti‐inhibin serum treatment combined with fixed‐time artificial insemination

Jia

Chen

Zhu³

et al. 2020

Reprod Domestic Animals

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For successful batch farrowing, porcine oestrus and ovulation must be synchronized using fixed‐time artificial insemination (FTAI). However, exogenous gonadotropins, which are currently used in FTAI, negatively affect gilt ovulation. Here, we aimed to improve sexually mature gilt superovulation efficiency using passive immunization against inhibin during FTAI. Altrenogest‐treated gilts were challenged with 10 ml anti‐inhibin serum (AIS group, n = 6), 1,000 IU pregnant mare serum gonadotropin (PMSG group, n = 6), or 10 ml goat serum (control group, n = 6). Gilts in the AIS and PMSG groups were inseminated according to the FTAI protocol, and gilts in the control group were inseminated during natural oestrus. When PMSG was replaced by AIS during FTAI of gilts, ovulation rate and embryos recovered were significantly greater in the AIS group as compared to the other two groups (p < .05). Especially the average number of 6–8‐cell embryos in the AIS group was significantly higher than that in the PMSG group (p < .01). Moreover, the blastocyst number in the AIS group was significantly higher than that in the PMSG group and the control group (p < .05). But there was no significant difference in the blastocyst number between the PMSG group and the control group (p > .05). Besides, plasma levels of estradiol‐β (E2) and progesterone (P4) were significantly greater in the AIS group as compared to the other two groups on Day 23 and D 27, respectively (p < .01). In summary, we devised an improved high‐yield FTAI protocol for sexually mature gilts using AIS; this protocol had a greater superovulation efficiency than the FTAI using PMSG.

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“…DNA vaccines have been widely used for various diseases, including avian influenza virus (Pan et al ., ) and HIV (Tuomela et al ., ). Immunization against the inhibin α‐subunit, which is the functional centre of the heterodimeric protein, increases the serum FSH concentration and, consequently, follicle development in mice (Medan et al ., ), goats (Wang et al ., ), guinea pigs (Shi et al ., ) and heifers (Akagi et al ., ). Therefore, the inhibin α‐subunit may be a potential novel DNA vaccine target for enhancing ovulation in animals.…”

Section: Introductionmentioning

confidence: 99%

The efficacy, biodistribution and safety of an inhibin DNA vaccine delivered by attenuated Salmonella choleraesuis

Chen

Li²,

Xue

et al. 2017

Microbial Biotechnology

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Summary DNA vaccines, the third‐generation vaccines, were extensively studied. The attenuated Salmonella choleraesuis (S. choleraesuis) was widely focused as a carrier to deliver DNA vaccines in the chromosome–plasmid balanced‐lethal system. The efficacy of inhibin DNA vaccine delivered by attenuated S. choleraesuis was proved in mice and cows in our previous studies. In this study, the efficacy of inhibin DNA vaccine was confirmed in rhesus monkeys. To further study the biodistribution and safety, the mice were immunized under laboratory conditions. The results of the rhesus monkeys showed the plasma IgA and IgG titres against inhibin were elevated, and the oestradiol (E2) and progesterone (P4) levels were increased with immunizing inhibin DNA vaccine. The biodistribution and safety assessment displayed the body weight, pathological change and haematology indexes where there is no significant difference between vaccinated mice and control. And the genomics analysis showed there was no integration of the inhibin gene into the mouse genome 2 months after immunization. This study indicated the inhibin DNA vaccine delivered by attenuated S. choleraesuis was safe. And this vaccine was a potential means to improve their reproductive traits in primates and other animals.

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The long-term effect of active immunization against inhibin in goats

Cited by 18 publications

References 25 publications

‘Free’ inhibin α subunit is expressed by bovine ovarian theca cells and its knockdown suppresses androgen production

‘Free’ inhibin α subunit is expressed by bovine ovarian theca cells and its knockdown suppresses androgen production

Improving ovulation in gilts using anti‐inhibin serum treatment combined with fixed‐time artificial insemination

The efficacy, biodistribution and safety of an inhibin DNA vaccine delivered by attenuated Salmonella choleraesuis

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