The long-term administration of calcineurin inhibitors decreases antioxidant enzyme activity in the rat parotid and submandibular salivary glands

Spolidório, Luís Carlos; Herrera, Bruno S.; Coimbra, Leila S.; Andrade, Cléverton Roberto de; Spolidório, Denise Madalena Palomari; Rossa, Carlos; Muscará, Marcelo Nícolas

doi:10.1016/j.lfs.2015.04.022

Cited by 10 publications

(17 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the liver transplant recipients, one in three patients with chronic liver disease had reduced salivary flow, and more than half of all patients positive for Candida . Although this risk was higher with steroids, we did not assess the use of other medications that might have interfered with this outcome. The high frequency of these oral manifestations among renal transplant recipients can be explained not only by immunosuppressive drugs but also by other medications.…”

Section: Discussionsupporting

confidence: 89%

“…Tacrolimus is a calcineurin inhibitor widely used for the prevention of graft rejection, resulting in increased longevity and enhanced quality of life for transplant patients. The systemic long‐term administration of calcineurin inhibitor cyclosporine A (CsA) or tacrolimus induces an impairment of the antioxidant enzymatic defense in the major salivary glands of rats, which could lead to altered saliva composition . To the best of our knowledge, there are no reports on the association between decreased salivary flow and the use of tacrolimus.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Salivary shedding of herpesviruses in renal transplant recipients

Sarmento

Caliento

Souza

et al. 2018

J of Invest & Clin Dent

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Salivary shedding of herpesviruses in renal transplant recipients

Sarmento

Caliento

Souza

et al. 2018

J of Invest & Clin Dent

View full text Add to dashboard Cite

show abstract

“…TBARS assay was used as a rapid screening procedure [34], since it is one of the most classical methods for determining oxidative damage in tissues and homogenates [35,36], plasma [37], salivary glands [38], and also in saliva [12,25]. This study demonstrated that salivary TBARS concentrations gradually decreased in children with cavitated lesions (enamel and dentin) as compared to caries-free children or those with initial lesions.…”

Section: Discussionmentioning

confidence: 90%

Carious Lesion Severity Induces Higher Antioxidant System Activity and Consequently Reduces Oxidative Damage in Children’s Saliva

Araujo

Nakamune

Garcia

et al. 2020

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Oxidative stress biomarkers can be found at detectable concentrations in saliva. These salivary biomarkers reflect specific oxidation pathways associated with caries and periodontitis. Our study evaluated the influence of dental caries severity (assessed using the ICCMS™ criteria) on the levels of oxidative stress biomarkers in saliva from children. Unstimulated saliva samples were collected from patients (from one to three years old) in a day care center in Birigui, SP, Brazil, two hours after fasting. Children were divided into four groups (n=30/group), according to caries severity: caries free (group A), early carious lesions (group B), moderate carious lesions (group C), and advanced carious lesions (group D). The following salivary biomarkers were determined: total proteins (TP), measured by the Lowry method; oxidative damage, measured by the TBARS method; total antioxidant capacity (TAC); superoxide dismutase (SOD) enzymatic antioxidant activity; and uric acid (UA) non-enzymatic antioxidant activity. Data were analyzed by ANOVA, followed by the Student-Newman-Keuls test, Pearson and Spearman correlation coefficients, and multivariable linear regression (p<0.05). TP, TAC, SOD enzymatic antioxidant activity, and UA non-enzymatic antioxidant activity increased with caries severity, consequently reducing salivary oxidative damage. It was concluded that higher caries severity increases salivary antioxidant system activity, with consequent reduction in salivary oxidative damage.

show abstract

“…Additionally, we cannot rule out longer treatment of rolipram. It has been proposed that long-term administration up to 2 months of calcineurin inhibitors decreases antioxidant enzyme activity and alters saliva composition [ 38 ]. Similarly, the administration of high dose of PDE inhibitor ICI 153,110 for up to 6 months influenced the epithelial cell proliferation including salivary glands [ 39 ].…”

Section: Discussionmentioning

confidence: 99%

The Regulatory Role of Rolipram on Inflammatory Mediators and Cholinergic/Adrenergic Stimulation-Induced Signals in Isolated Primary Mouse Submandibular Gland Cells

Lee

Shin

Hong

2016

Mediators of Inflammation

View full text Add to dashboard Cite

Exposure to bacterial lipopolysaccharides (LPS) induces inflammatory signals in salivary glands. We investigated the regulatory role of phosphodiesterase 4 (PDE4) inhibitor rolipram on inflammatory mediators and cholinergic/adrenergic stimulation-induced intracellular Ca2+ signaling in salivary acinar and ductal cells. Submandibular gland (SMG) expressed PDE4A through 4D mRNA and PDE4 was localized in the luminal membrane of SMG. LPS induced Ca2+ signaling and ROS production in SMG. Treatment with rolipram blocked LPS-induced Ca2+ increase and ROS production. The application of histamine evoked Ca2+ signals and ROS production, which were attenuated by rolipram in SMG cells. Moreover, LPS-induced NLRP3 inflammasome and cleaved caspase-1 were inhibited by rolipram. The inhibitory role of rolipram in ROS-induced Ca2+ signaling was mainly observed in acinar cells and not in ductal cells. Rolipram also protected SMG acinar but not ductal cells from LPS-induced cell membrane damage. In the case of cholinergic/adrenergic stimulation, carbachol/isoproterenol-induced Ca2+ signals were upregulated by the treatment of rolipram in SMG. In the case of cAMP-dependent ductal bicarbonate secretion by rolipram, no effect was observed on the modulation of ductal chloride/bicarbonate exchange activity. Rolipram could suppress the inflammatory signals and could be a potential therapeutic strategy against LPS-induced inflammation to protect the salivary gland cells.

show abstract

The long-term administration of calcineurin inhibitors decreases antioxidant enzyme activity in the rat parotid and submandibular salivary glands

Cited by 10 publications

References 54 publications

Salivary shedding of herpesviruses in renal transplant recipients

Salivary shedding of herpesviruses in renal transplant recipients

Carious Lesion Severity Induces Higher Antioxidant System Activity and Consequently Reduces Oxidative Damage in Children’s Saliva

The Regulatory Role of Rolipram on Inflammatory Mediators and Cholinergic/Adrenergic Stimulation-Induced Signals in Isolated Primary Mouse Submandibular Gland Cells

Contact Info

Product

Resources

About