The long noncoding RNA AATBC promotes breast cancer migration and invasion by interacting with YBX1 and activating the YAP1/Hippo signaling pathway

Abstract

BackgroundLong noncoding RNAs (lncRNAs) play an important role in the regulation of gene expression and are involved in several pathological responses. However, many important lncRNAs in breast cancer have not been identified and their expression levels and functions in breast cancer remain unknown.MethodsWe used the microarray data to identify differentially expressed lncRNAs between breast cancer and adjacent breast epithelial tissues. In vitro and in vivo assays were used to explore the biological effects of the differentially expressed lncRNA Apoptosis-Associated Transcript in Bladder Cancer (AATBC) in breast cancer cells. The mass spectrometry and RNA pulldown were used to screen AATBC interacting proteins. Using the Kaplan-Meier method, survival analysis was performed.ResultsThe expression of AATBC was significantly high in breast cancer samples, and this high AATBC level was tightly correlated with poor prognosis in breast cancer patients. In vitro and in vivo experiments indicated that AATBC promoted breast cancer cells migration and invasion. AATBC specifically interacted with Y-box binding protein 1 (YBX1), which activated the YAP1/Hippo signaling pathway by binding to macrophage stimulating 1 (MST1) and promoting the nuclear translocation of Yes associated protein 1 (YAP1), allowing its function as a nuclear transcriptional regulator. ConclusionsAATBC is highly expressed in breast cancer and contributes to patients’ progression, indicating that it could serve as a novel prognostic marker for the disease. Mechanistically, AATBC affects migration and invasion of breast cancer cells through an AATBC-YBX1-MST1 axis, resulting in activating the YAP1/Hippo signaling pathway. This is also an important supplement to the composition of the YAP1/Hippo signaling pathway. The model of “AATBC-YAP1” may bring a new dawn to the treatment of breast cancer.

LncRNA AATBC indicates development and facilitates cell growth and metastasis of cervical cancer as a sponge of miR‐1245b‐5p

“…For instance, upregulation of AATBC was observed in breast cancer, which accelerates the metastasis of breast cancer by negatively modulating YBX1 and activating the YAP1/ Hippo signaling pathway. 13 Similarly, a promoted effect of AATBC has been observed in the proliferation and migration of prostate cancer cells via the regulation of the miR-1245b-5p/CASK axis. 14 Based on previous findings that AATBC is a dysregulated lncRNA in cervical cancer, its potential functional role in cervical cancer was evaluated in the present study.…”

“…In a recent study, Li et al screened 130 dysregulated lncRNAs in cervical cancer using the lncRNA microarray analysis, wherein the alerting expression of lncRNA AATBC (AATBC) was observed 11 . AATBC was suggested to regulate the progression of various human cancers, such as nasopharyngeal carcinoma, prostate cancer, breast cancer, and bladder cancer; AATBC was also found to be dysregulated in each of these conditions 12–15 …”

“…11 AATBC was suggested to regulate the progression of various human cancers, such as nasopharyngeal carcinoma, prostate cancer, breast cancer, and bladder cancer; AATBC was also found to be dysregulated in each of these conditions. [12][13][14][15] Hence, the present study examined whether AATBC is differentially expressed in cervical cancer and whether it participates in tumor progression. This study aimed to (1) clarify the expression of AATBC in cervical cancer; (2) explore the significance of AATBC in the development of cervical cancer and patients' prognosis; and (3) assess the function of AATBC in regulating cellular processes and disclose the potential molecular mechanism.…”

High Expression of lncRNA HEIH is Helpful in the Diagnosis of Non-Small Cell Lung Cancer and Predicts Poor Prognosis

“…LncRNAs are important in gene regulation. 33 LncRNA interacts with many transcription factors and affects lung cancer growth and spread. 13,34 To date, many lncRNAs have been shown to be associated with the diagnosis and prognosis of NSCLC.…”

Dysregulation of tumor microenvironment promotes malignant progression and predicts risk of metastasis in bladder cancer

“…Cellular level QPCR results showed that six TMErelated prognostic genes were dysregulated in expression between BCa cells and normal bladder epithelium, suggesting that dysregulated expression levels of these genes play an important role in the development of BCa. Zhang et al (44) reported that GATA3-AS1 promoted the development and metastasis of triple-negative breast cancer by stabilizing tumor immune escape, while Wang et al (45) found AATBC was closely associated with breast cancer metastasis. Further, Sun et al (46) found that TGR-AS1 promoted hepatocellular carcinoma invasion and metastasis by regulating the EMT pathway, and Zhang et al (47) found that LINC01833 promoted EMT function.…”