The long non-coding RNA SNHG5 regulates gefitinib resistance in lung adenocarcinoma cells by targetting <i>miR-377</i>/CASP1 axis

Wang, Zhenxing; Pan, Liming; Yu, Haixiang; Wang, Yue

doi:10.1042/bsr20180400

Cited by 25 publications

(10 citation statements)

References 19 publications

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“…However, the responsiveness of different HCC patients to gefitinib varies greatly and most patients even develop gefitinib resistance [ 60 ]. Multiple non-coding RNAs including miRNAs and lncRNAs have been reported to contribute to gefitinib resistance and sensitivity in NSCLC [ 61 – 63 ]. We speculate that gefitinib share the similar resistance mechanisms in HCC and lung cancer.…”

Section: Discussionmentioning

confidence: 99%

A circRNA–miRNA–mRNA network identification for exploring underlying pathogenesis and therapy strategy of hepatocellular carcinoma

et al. 2018

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BackgroundCircular RNAs (circRNAs) have received increasing attention in human tumor research. However, there are still a large number of unknown circRNAs that need to be deciphered. The aim of this study is to unearth novel circRNAs as well as their action mechanisms in hepatocellular carcinoma (HCC).MethodsA combinative strategy of big data mining, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and computational biology was employed to dig HCC-related circRNAs and to explore their potential action mechanisms. A connectivity map (CMap) analysis was conducted to identify potential therapeutic agents for HCC.ResultsSix differently expressed circRNAs were obtained from three Gene Expression Omnibus microarray datasets (GSE78520, GSE94508 and GSE97332) using the RobustRankAggreg method. Following the RT-qPCR corroboration, three circRNAs (hsa_circRNA_102166, hsa_circRNA_100291 and hsa_circRNA_104515) were selected for further analysis. miRNA response elements of the three circRNAs were predicted. Five circRNA–miRNA interactions including two circRNAs (hsa_circRNA_104515 and hsa_circRNA_100291) and five miRNAs (hsa-miR-1303, hsa-miR-142-5p, hsa-miR-877-5p, hsa-miR-583 and hsa-miR-1276) were identified. Then, 1424 target genes of the above five miRNAs and 3278 differently expressed genes (DEGs) on HCC were collected. By intersecting the miRNA target genes and the DEGs, we acquired 172 overlapped genes. A protein–protein interaction network based on the 172 genes was established, with seven hubgenes (JUN, MYCN, AR, ESR1, FOXO1, IGF1 and CD34) determined from the network. The Gene Oncology, Kyoto Encyclopedia of Genes and Genomes and Reactome enrichment analyses revealed that the seven hubgenes were linked with some cancer-related biological functions and pathways. Additionally, three bioactive chemicals (decitabine, BW-B70C and gefitinib) based on the seven hubgenes were identified as therapeutic options for HCC by the CMap analysis.ConclusionsOur study provides a novel insight into the pathogenesis and therapy of HCC from the circRNA–miRNA–mRNA network view.

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Section: Discussionmentioning

confidence: 99%

A circRNA–miRNA–mRNA network identification for exploring underlying pathogenesis and therapy strategy of hepatocellular carcinoma

et al. 2018

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“…lncRNAs are a type of ncRNA and play an important role in the tumorigenesis process. 9 lncRNA ANRIL is overexpressed in osteosarcoma and lung cancer; however, the role and biological mechanism of lncRNA ANRIL in glioma have not been fully understood. We found that there was an increased expression of lncRNA ANRIL in glioma tissue samples, positively correlated with grade.…”

Section: Discussionmentioning

confidence: 99%

Effect of lncRNA ANRIL silencing on anoikis and cell cycle in human glioma via microRNA-203a

Dai¹,

Tian²,

Jin³

2018

OTT

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BackgroundGlioma is a deadly nervous system tumor with a poor prognosis. Although there have been many efforts to overcome glioma, the molecular mechanism of its pathogenesis remains unclear.MethodsWe used human glioma U251 cells silenced for the oncogenic lncRNA ANRIL or overexpressing the anti-oncogene miR-203a to examine the role of lncRNA ANRIL silencing on anoikis and cell cycle arrest by flow cytometry. Meanwhile, the activity of caspase-3/8/9 was measured by fluorometric assay, the expression of tumor-related genes and activity of AKT signaling pathway was measured by Western blotting, real-time PCR, and dual luciferase reporter gene assay.ResultslncRNA ANRIL was positively correlated with glioma grade and negatively correlated with miR-203a. lncRNA ANRIL silencing could induce anoikis and cell cycle arrest in G0/G1 phase, while regulating the activity of caspase-3/8/9 and the AKT signaling pathway, and the expression of tumor-related genes in the U251 cell line. miR-203a mimics could partially reverse these functions.ConclusionWe consider that lncRNA ANRIL is a potential therapeutic and diagnostic target for glioma, and miR-203a plays an important role in the biological function of lncRNA ANRIL in glioma.

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“…It was formerly reported that SNHG5 is functionally related to many kinds of tumors, including chronic myeloid leukemia [ 8 ], gastric cancer [ 7 , 9 ], colorectal cancer [ 10 ], melanoma [ 11 ], and bladder cancer [ 12 ]. In addition, a recent document proclaimed that the overexpression of SNHG5 sensitized LAD cells to gefitinib treatment by interacting with miR-377 to modulate its downstream target, CASP1 [ 5 ]. However, the functions and molecular basis of SNHG5 in LAD cells and their correlation with LAD pathogenesis have not been further explored.…”

Section: Introductionmentioning

confidence: 99%

LncRNA SNHG5 Suppresses Cell Migration and Invasion of Human Lung Adenocarcinoma via Regulation of Epithelial-Mesenchymal Transition

Zhang

et al. 2023

Journal of Oncology

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Long noncoding RNAs (lncRNAs) are gradually being annotated as important regulators of multiple cellular processes. The goal of our study was to investigate the effects of the lncRNA small nucleolar RNA host gene 5 (SNHG5) in lung adenocarcinoma (LAD) and its underlying mechanisms. The findings revealed a substantial drop in SNHG5 expression in LAD tissues, which correlated with clinical-pathological parameters. Transcriptome sequencing analysis demonstrated that the inhibitory effect of SNHG5 was associated with cell adhesion molecules. Moreover, the expression of SNHG5 was shown to be correlated with epithelial–mesenchymal transition (EMT) markers in western blots and immunofluorescence. SNHG5 also had significant effects of antimigration and anti-invasion on LAD cells in vitro. Furthermore, the migration and invasion of A549 cells were suppressed by overexpressed SNHG5 in the EMT progress induced by transforming growth factor β1 (TGF-β1), and this might be due to the inhibition of the expression of EMT-associated transcription factors involving Snail, SLUG, and ZEB1. In LAD tissues, the expression of SNHG5 exhibited a positive association with E-cadherin protein expression but a negative correlation with N-cadherin and vimentin, according to the results of quantitative real-time PCR (qRT-PCR). In summary, the current work demonstrated that the lncRNA SNHG5 might limit cell migration and invasion of LAD cancer via decreasing the EMT process, indicating that SNHG5 might be used as a target for LAD therapeutic methods.

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The long non-coding RNA SNHG5 regulates gefitinib resistance in lung adenocarcinoma cells by targetting miR-377/CASP1 axis

Cited by 25 publications

References 19 publications

A circRNA–miRNA–mRNA network identification for exploring underlying pathogenesis and therapy strategy of hepatocellular carcinoma

A circRNA–miRNA–mRNA network identification for exploring underlying pathogenesis and therapy strategy of hepatocellular carcinoma

Effect of lncRNA ANRIL silencing on anoikis and cell cycle in human glioma via microRNA-203a

LncRNA SNHG5 Suppresses Cell Migration and Invasion of Human Lung Adenocarcinoma via Regulation of Epithelial-Mesenchymal Transition

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