The long non-coding RNA HOXA11-AS promotes epithelial mesenchymal transition by sponging miR-149-3p in Colorectal Cancer

Chen, Dong; Zhang, Min; Ruan, Jian; Li, Xin; Wang, Saisai; Cheng, Xiaofei; Zhao, Huiying; Zeng, Ying; Liu, Jingjing; He, Kangxin; Zhao, Peng

doi:10.7150/jca.49809

Cited by 20 publications

(12 citation statements)

References 50 publications

(65 reference statements)

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“…23 Furthermore, miR-149-3p could serve as an oncogene, thereby promoting the proliferation of tumor cells and limiting the apoptosis of tumor cells. 24,25 In another study, the suppressive effect of tumors was documented in other cancer types, such as bladder cancer, 9 colorectal cancer 26 and oral squamous cell carcinoma. 27 It thus appears that miR-149-5p and miR-149-3p may exert neither antergic or synergetic function in the progression of a cancer.…”

Section: Discussionmentioning

confidence: 98%

Hsa-miR-149-5p Suppresses Prostate Carcinoma Malignancy by Suppressing RGS17

Ma¹,

Wei²,

Li³

et al. 2021

CMAR

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Background MicroRNAs (miRNAs) are key players in the progression of human cancers. While several miRNAs have been reported to regulate the development of tumors, the molecular mechanisms and roles of miR-149-5p in prostate carcinoma (PCa) remain unclear. Our aim was to investigate the interaction and functions of miR-149-5p and RGS17 in PCa. Methods Microarray analysis was performed to identify the key miRNA and gene involved in PCa progression. The expression levels of miRNA and mRNA in PCa tissues and cells were verified by qRT-PCR. MTT assay, BrdU proliferation assay and wound-healing assay were applied to assess the effect of miR-149-5p and RGS17 on PCa cells’ viability, proliferation, and migration ability. The association between RGS17 and miR-149-5p was identify using dual-luciferase reporter assay and Western blot assay. Results Data analysis indicated the reduction of miR-149-5p expression in PCa tissues and cells. Experimental investigations also showed that this miRNA suppressed the viability, proliferation and migration ability of PCa cells. RGS17 was found to be the target of miR-149-5p, and the low expression of miR-149-5p upregulated RGS17 in PCa tissues and cells. The results of the cell-function assays showed that RGS17 acted as an oncogene in PCa even though its promotive effect could be reversed by miR-149-5p. Conclusion This research confirmed that by targeting and inhibiting RGS17, miR-149-5p could suppress PCa development.

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Section: Discussionmentioning

confidence: 98%

Hsa-miR-149-5p Suppresses Prostate Carcinoma Malignancy by Suppressing RGS17

Ma¹,

Wei²,

Li³

et al. 2021

CMAR

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“…There are even fewer studies analyzing methylation patterns of HOXA11 in GI cancers. Several overexpression and knockdown studies have shown that HOXA11 is involved in regulating the liver metastasis in colorectal cancer cell lines [ 21 ]. In some previous studies, several pathways of HOXA11 functions in the development and progression of hepatocellular carcinoma (HCC) were analyzed.…”

Section: Discussionmentioning

confidence: 99%

DNA Methylation of HOXA11 Gene as Prognostic Molecular Marker in Human Gastric Adenocarcinoma

et al. 2022

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Hypermethylation of tumor suppressor genes and hypomethylation of oncogenes might be identified as possible biomarkers in gastric cancer (GC). We aimed to assess the DNA methylation status of selected genes in GC tissue samples and evaluate these genes’ prognostic importance on patient survival. Patients (99) diagnosed with GC and who underwent gastrectomy were included. We selected a group of genes (RAD51B, GFRA3, AKR7A3, HOXA11, TUSC3, FLI1, SEZ6L, GLDC, NDRG) which may be considered as potential tumor suppressor genes and oncogenes. Methylation of the HOXA11 gene promoter was significantly more frequent in GC tumor tissue (p = 0.006) than in healthy gastric mucosa. The probability of surviving longer (71.2 months (95% CI 57–85.3) vs. 44.3 months (95% CI 34.8–53.9)) was observed with unmethylated HOXA11 promoter in cancer tissues. Survival in patients with a methylation of HOXA11 promoter either in healthy gastric mucosa or gastric cancer tissue was twice as high as in patients with a methylation of HOXA11 promoter in both healthy gastric mucosa and cancer tissue (61.2 months (95% CI 50.9–71.4) vs. 28.5 months (95% CI 20.8–36.2)). Multivariate Cox analysis revealed the HOXA11 methylation as significantly associated with patients’ survival (HR = 2.4, 95% CI 1.19–4.86). Our results suggest that the HOXA11 gene might be a potential prognostic molecular marker in patients with gastric adenocarcinoma.

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“…Besides, miR-124-3p was defined as another molecular targeted by HOXA11-AS to promote malignant progression of glioma [ 28 ]. In osteosarcoma, hepatocellular carcinoma and colorectal cancer (CRC) studies, HOXA11-AS has been identified as a ceRNA to regulate gene expression by sponging specific miRNA to promote tumor progression [ 8 , 29 – 31 ]. In the fracture healing studies, lncRNA HOXA11-AS can inhibit osteoblast proliferation and promote osteoblast apoptosis [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…A preliminary mechanism study by database analysis and western blot analysis showed SRSF1 may be the target of HOXA11-AS in GC [ 23 ]. In addition, effective mechanisms of HOXA11-AS in other tumors were far more complicated, such as miR-149-3p/epithelial mesenchymal transition (EMT) pathway in CRC [ 29 ] and miR-a24-3p/ROCK1 in osteosarcoma [ 8 ]. In fact, cancer initiation and progression are indeed the complex biological processes concerning the activating or inhibiting of a series of molecules and pathways.…”

Section: Discussionmentioning

confidence: 99%

The long non-coding RNA HOXA11-AS activates ITGB3 expression to promote the migration and invasion of gastric cancer by sponging miR-124-3p

You

Xin

et al. 2021

Cancer Cell Int

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Background miR-124-3p can inhibit integrin β3 (ITGB3) expression to suppress the migration and invasion of gastric cancer (GC), and in the process lncRNA HOXA11-AS may act as a molecular sponge. Methods Luciferase reporter assay was conducted to verify the binding of miR-124-3p and HOXA11-AS. RT-PCR and western blot were performed to detect the expression of HOXA11-AS, miR-124-3p and ITGB3 in GC tissues and cells. Gene silence and overexpression experiments as well as cell migration and invasion assays on GC cell lines were performed to determine the regulation of molecular pathways, HOXA11-AS/miR-124-3p/ITGB3. Furthermore, the role of HOXA11-AS in GC was confirmed in mice models. Results We found HOXA11-AS is up-regulated in GC tissues and can bind with miR-124-3p. Through overexpression/knockdown experiments and function tests in vitro, we demonstrated HOXA11-AS can promote ITGB3 expression by sponging miR-124-3p, consequently enhance the proliferation, migration, and invasion of GC cells. Meanwhile, we validated that HOXA11-AS promotes migration and invasion of GC cells via down-regulating miR-124-3p and up-regulating ITGB3 in vivo. Conclusions We demonstrated that lncRNA HOXA11-AS can increase ITGB3 expression to promote the migration and invasion of gastric cancer by sponging miR-124-3p. Our results suggested that HOXA11-AS may reasonably serve as a promising diagnostic biomarker and a potential therapeutic target of GC.

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The long non-coding RNA HOXA11-AS promotes epithelial mesenchymal transition by sponging miR-149-3p in Colorectal Cancer

Cited by 20 publications

References 50 publications

Hsa-miR-149-5p Suppresses Prostate Carcinoma Malignancy by Suppressing RGS17

Hsa-miR-149-5p Suppresses Prostate Carcinoma Malignancy by Suppressing RGS17

DNA Methylation of HOXA11 Gene as Prognostic Molecular Marker in Human Gastric Adenocarcinoma

The long non-coding RNA HOXA11-AS activates ITGB3 expression to promote the migration and invasion of gastric cancer by sponging miR-124-3p

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