The Long and Short of MicroRNA

Yates, Luke A.; Norbury, Chris J.; Gilbert, Robert J.C.

doi:10.1016/j.cell.2013.04.003

Cited by 636 publications

(482 citation statements)

References 21 publications

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“…M icroRNAs (miRNAs) are small (17-24 bp), noncoding RNAs that regulate different biological functions by modulating expression and stability of target genes posttranscriptionally (1,2). The role of miRNAs in lung development is currently poorly defined.…”

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confidence: 99%

The transcriptome of nitrofen-induced pulmonary hypoplasia in the rat model of congenital diaphragmatic hernia

Mahood

Johar

et al. 2015

Pediatr Res

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Background:We currently do not know how the herbicide nitrofen induces lung hypoplasia and congenital diaphragmatic hernia in rats. Our aim was to compare the differentially expressed transcriptome of nitrofen-induced hypoplastic lungs to control lungs in embryonic day 13 rat embryos before the development of embryonic diaphragmatic defects. Methods: Using next-generation sequencing technology, we identified the expression profile of microRNA (miRNA) and mRNA genes. Once the dataset was validated by both RT-qPCR and digital-PCR, we conducted gene ontology, miRNA target analysis, and orthologous miRNA sequence matching for the deregulated miRNAs in silico. results: Our study identified 186 known mRNA and 100 miRNAs which were differentially expressed in nitrofeninduced hypoplastic lungs. Sixty-four rat miRNAs homologous to known human miRNAs were identified. A subset of these genes may promote lung hypoplasia in rat and/or human, and we discuss their associations. Potential miRNA pathways relevant to nitrofen-induced lung hypoplasia include PI3K, TGF-β, and cell cycle kinases. conclusion: Nitrofen-induced hypoplastic lungs have an abnormal transcriptome that may lead to impaired development.

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confidence: 99%

The transcriptome of nitrofen-induced pulmonary hypoplasia in the rat model of congenital diaphragmatic hernia

Mahood

Johar

et al. 2015

Pediatr Res

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“…24 miRNAs are involved in the regulation of various cell processes including development, apoptosis, proliferation, and differentiation. 1 Further investigations have confirmed that miRNAs can function as tumor suppressors or oncogenes and play essential roles in a wide variety of physiological and pathological processes in various cancers, including glioma.…”

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confidence: 99%

Downregulation of serum microRNA-205 as a potential diagnostic and prognostic biomarker for human glioma

Xiao

Lan

et al. 2016

JNS

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G lioma is the most common primary intracranial tumor in adults, accounting for more than 40% of primary CNS neoplasms. In accordance with WHO classification, which is based on histomorphological criteria, gliomas are categorized as well-differentiated low-grade astrocytoma (WHO Grade I or II), anaplastic astrocytoma (WHO Grade III), or glioblastoma (GBM; WHO Grade IV). 15 The most frequent and malignant glioabbreviatioNs GBM = glioblastoma; KPS = Karnofsky Performance Scale; miR-205 = microRNA 205; miRNA = microRNA; OS = overall survival; PCNSL = primary diffuse large B-cell lymphoma of the CNS; qRT-PCR = quantitative reverse-transcription polymerase chain reaction. obJective Circulating microRNAs (miRNAs) are a new class of highly promising cancer biomarkers. Malignant glioma is one of the most devastating and lethal forms of intrinsic CNS tumor. Here, the authors evaluated serum miRNA 205 (miR-205) levels in patients with glioma. methods Sixty-four patients in whom glioma was diagnosed and 45 healthy controls were recruited between October 2011 and March 2012 and randomly assigned to the screening cohort or the validation cohort. Cohorts of patients with other brain tumors, including meningioma (n = 8), primary diffuse large B-cell lymphoma of the CNS (n = 6), and pituitary adenoma (n = 5), were investigated and compared. miR-205 extraction from serum was detected by real-time quantitative reverse-transcription polymerase chain reaction. The Kaplan-Meier method was applied to perform survival analysis, the risk factors were analyzed by using a Cox regression model, and the receiver operating characteristic working curve was used to analyze the value of miR-205 in the prognostic evaluation of the patients. results The authors first demonstrated that serum miR-205 expression was significantly lower in patients with glioma than in healthy controls (p < 0.001). It is important to note that serum miR-205 expression demonstrated a stepwise decrease with ascending pathological grades. The serum miR-205 biomarker had high sensitivity, specificity, and accuracy in patients with glioma. Serum levels of miR-205 were identified as an individual diagnostic marker and were significantly lower in the glioma cohort than in the other brain tumor cohorts. Serum miR-205 levels were significantly increased in postoperative samples over those in the preoperative samples and were reduced again during glioblastoma recurrences. Statistical analysis revealed a significant correlation between low serum miR-205 expression and both ascending pathological grades (p = 0.002) and low Karnofsky Performance Scale scores (p = 0.01). Patients with glioma at an advanced pathological grade (Grade III or IV) and a higher miR-205 serum level showed longer overall survival than those with a lower miR-205 serum concentration (p < 0.01). Furthermore, Cox regression analysis revealed that miR-205 serum levels were independently associated with overall survival. coNclusioNs These data indicate that serum miR-205 expression is a novel and valuable bioma...

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“…In recent years, many miRNAs targeting complementary sequences in 3 0 UTR have been discovered, and evidence has much increased showing their importance in regulating physiologic and pathologic cellular pathways (36). As camptothecin treatment determines strong modification of transcription of several mRNAs (30,37,38), we questioned if the drug could modulate the miRNAs expression profiles in two human cancer cell lines and, if that was the case, if miRNAs modifications reflect in the HIF-1a protein expression.…”

Section: Discussionmentioning

confidence: 99%

The Natural Inhibitor of DNA Topoisomerase I, Camptothecin, Modulates HIF-1α Activity by Changing miR Expression Patterns in Human Cancer Cells

Bertozzi

Marinello

Manzo

et al. 2014

Molecular Cancer Therapeutics

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DNA topoisomerase I (Top1) inhibition by camptothecin derivatives can impair the hypoxia-induced cell transcriptional response. In the present work, we determined molecular aspects of the mechanism of camptothecin's effects on hypoxia-inducible factor-1a (HIF-1a) activity in human cancer cells. In particular, we provide evidence that low concentrations of camptothecin, without interfering with HIF-1a mRNA levels, can reduce HIF-1a protein expression and activity. As luciferase assays demonstrated the involvement of the HIF-1a mRNA 3 0 untranslated region in camptothecin-induced impairment of HIF-1aprotein regulation, we performed microarray analysis to identify camptothecin-induced modification of microRNAs (miRNA) targeting HIF-1a mRNA under hypoxic-mimetic conditions. The selected miRNAs were then further analyzed, demonstrating a role for miR-17-5p and miR-155 in HIF-1a protein expression after camptothecin treatments. The present findings establish miRNAs as key factors in a molecular pathway connecting Top1 inhibition and human HIF-1a protein regulation and activity, widening the biologic and molecular activity of camptothecin derivatives and the perspective for novel clinical interventions. Mol Cancer Ther; 13(1); 239-48. Ó2013 AACR.

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The Long and Short of MicroRNA

Cited by 636 publications

References 21 publications

The transcriptome of nitrofen-induced pulmonary hypoplasia in the rat model of congenital diaphragmatic hernia

The transcriptome of nitrofen-induced pulmonary hypoplasia in the rat model of congenital diaphragmatic hernia

Downregulation of serum microRNA-205 as a potential diagnostic and prognostic biomarker for human glioma

The Natural Inhibitor of DNA Topoisomerase I, Camptothecin, Modulates HIF-1α Activity by Changing miR Expression Patterns in Human Cancer Cells

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