The locus for resistance to Asian soybean rust in PI 587855

Abstract

Asian soybean rust (ASR) caused by Phakopsora pachyrhizi is one of the most serious soybean (Glycine max) diseases in tropical and subtropical areas. A soybean line, PI 587855, showed a resistance phenotype against ASR pathogens in Japan and South America at high frequency; however, little is known of the genetic control of this resistance and chromosomal location of the corresponding locus. Therefore, the aim of this study was to study the inheritance of PI 587855 resistance and map the corresponding locus with SSR markers aiming to use the linked markers in marker‐assisted selection. In the segregating population, resistance to ASR appeared to be controlled by a single dominant gene. The ASR resistance locus was mapped near to the chromosomal region where the resistant loci, Rpp1 and Rpp1‐b, were previously mapped. Comparative genetic mapping and disease reaction profiles of other seven lines carrying Rpp1 or Rpp1‐b to four Brazilian ASR isolates revealed that the resistance reaction exhibited by PI 587855 was similar to that of Rpp1‐b‐carrying varieties which have useful resistance to South American ASR strains.

Novel disease resistance gene paralogs created by CRISPR/Cas9 in soy

“…Multiple closely linked ASR resistance genes have been genetically mapped to a region between the markers Satt191 and Sat_372 on chromosome 18 (Hossain et al 2015;Kim et al 2012;Liu et al 2016;Yamanaka et al 2016), which corresponds to 2,577,642 bp in physical distance (http:// www.soyba se.org) in the reference genome Williams 82 (W82). A four-copy NLR family of this region has been selected ( Fig.…”

“…poses a growing threat to global soybean production (Pivonia and Yang 2004). A region on the long arm of soy chromosome 18 harbors multiple closely linked resistance genes (Hossain et al 2015;Kim et al 2012;Liu et al 2016;Yamanaka et al 2016), which corresponds to approximately 2.5 Mbp in physical distance (http://www.soyba se.org). We selected a 4-copy tandem duplicated NLR complex in this region as one of our models and refer to it as Rpp1L (Rpp1-like) throughout this paper.…”

Breeding soybeans with resistance to soybean rust (Phakopsora pachyrhizi)

“…Additional studies have identified PIs with resistance genes mapping to the Rpp1 / Rpp1‐b locus, including PI 417120, PI 423958, PI 518295 and PI 594177 (Harris, Kendrick et al., ; Yamanaka, Hossain, & Yamaoka, ) which were obtained from Japan or Taiwan, and PI 561356, PI 587880A, PI 587886, PI 587905, PI 594760B, PI 594767A and PI 587855 obtained from China in the 1990s (Garcia, Calvo, Kiihl, & Souto, ; Hossain et al., ; Kim et al., ; Ray, Morel, Smith, Frederick, & Miles, ; Yamanaka et al., ). Although Akamatsu et al.…”

Confirmation of Rpp Genes Conferring Resistance to Asian Soybean Rust and Mapping of Rpp1 Allele from PI 594723

“…The Rpp1 (PI 200492) is highly resistant to Japanese and Mexican ASR isolates, but a lack of resistance has been reported to Brazilian ASR isolates (Aoyagi et al 2020;Akamatsu et al 2017;Hossain et al 2015). In contrast, the Rpp1-b (PI 587880A, PI 594538A) is resistant to most Brazilian ASR isolates (Ray et al 2009;Yamanaka et al 2016;Akamatsu et al 2017), highlighting a clear allelic difference between Rpp1 and Rpp1-b. Moreover, the PI 594723 hypothetically present Rpp1-b because of the phenotype similarity to PIs carrying Rpp1-b (Li, 2009).…”