The local administration of parathyroid hormone encourages the healing of bone defects in the rat calvaria: Micro-computed tomography, histological and histomorphometric evaluation

Auersvald, Caroline Moreira; Santos, Felipe Rychuv; Nakano, Mayara Mytie; Leoni, Graziela Bianchi; Sousa‐Neto, Manoel Damião; Scariot, Rafaela; Giovanini, Allan Fernando; Deliberador, Tatiana Miranda

doi:10.1016/j.archoralbio.2017.02.016

Cited by 18 publications

(18 citation statements)

References 24 publications

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“…The local administration of PTH has several advantages, such as convenience, without the need for repeated injections, and fewer side effects than systemic PTH injection. Although no study has evaluated the effect of tendon‐to‐bone healing by local PTH administration, its positive effects on bone healing processes have been previously reported . Auersvald et al showed that the local administration of PTH led to a good result in the healing of rat calvaria bone defects on micro‐computed tomography, histological, and histomorphometric evaluations.…”

Section: Discussionmentioning

confidence: 99%

“…Although no study has evaluated the effect of tendon‐to‐bone healing by local PTH administration, its positive effects on bone healing processes have been previously reported . Auersvald et al showed that the local administration of PTH led to a good result in the healing of rat calvaria bone defects on micro‐computed tomography, histological, and histomorphometric evaluations. Tokunaga et al reported that the topical and intermittent application of PTH recovered the alveolar bone loss in a rat experimental periodontitis.…”

Section: Discussionmentioning

confidence: 99%

“…The amount of PTH to be used in the experiment was determined based on the previous studies . Sodium alginate was dissolved in distilled water to prepare a 3% (w/v) solution, which was then added dropwise to a 5 w/v % CaCl 2 solution using low‐density polyethylene pipet.…”

Section: Methodsmentioning

confidence: 99%

“…In addition, the cost of repeated injection is high, and adverse events related with the systemic PTH injection include nausea, headaches, and leg cramps . The local administration of PTH was recently attempted to encourage bone defect healing in the rat model and was reported to promote fracture healing, increase bone mineral density, and the mechanical strength of new bone‐forming cells . Local PTH administration might be more convenient for patients as it can be conducted during surgery without repetition and may feature fewer side effects than the systemic injection.…”

mentioning

confidence: 99%

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Is a Local Administration of Parathyroid Hormone Effective to Tendon‐to‐Bone Healing in a Rat Rotator Cuff Repair Model?

Yoon

Chung

Jung

et al. 2019

Journal Orthopaedic Research

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To evaluate the effect of local parathyroid hormone (PTH) administration on rotator cuff tendon‐to‐bone healing in a rat model compared with systemic PTH injection and untreated controls. PTH‐alginate scaffold was prepared and sustained release of PTH was confirmed. Bilateral supraspinatus tendon repairs were performed in 39 rats (group 1, supraspinatus repair only; group 2, supraspinatus repair with systemic PTH injection; group 3, supraspinatus repair with local PTH administration via an absorbable scaffold; n = 13 each). Biomechanical (cross‐sectional area, mode of failure, load to failure, and ultimate stress: right side) and histological analyses (hematoxylin and eosin stain, Masson's Trichrome stain Picrosirius red stain, Immunohistochemistry for BMP2, PTH1R, ColI, and ColIII: Left side) were performed to evaluate tendon‐to‐bone healing quality at 8 weeks after repair, and blood test (osteocalcin and procollagen type I N‐terminal pro‐peptide [PINP] levels) was performed in all rats. There was no intergroup difference in the healing failure rate (p = 0.910) or failure mode (p = 0.585). Biomechanically, subjects in groups 2 and 3 exhibited significantly larger cross‐sectional areas and higher ultimate failure loads and ultimate stress than those in group 1 (all p < 0.05); however, no differences were noted between groups 2 and 3 (all p > 0.05). Histologically, groups 2 and 3 exhibited more organized tendon‐to‐bone interface structures with higher density, parallel orientation, and collagen fiber continuity than group 1 (all p < 0.05 except collagen fiber continuity in group 1 vs. 2); however, no differences in histological parameters between groups 2 and 3 (all p > 0.05). The protein levels of bone morphogenic protein 2, PTH 1 receptor, and collagen I and III and the serum level of PINP were increased in groups 2 and 3 versus group 1 (all p < 0.05) without showing differences between groups 2 and 3 (all p > 0.05). Local PTH administration using an absorbable scaffold improved the biomechanical and histological outcomes of rotator cuff tendon‐to‐bone healing comparable with systemic PTH injection at 8 weeks after repair in a rat model. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 38:82–91, 2020

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Is a Local Administration of Parathyroid Hormone Effective to Tendon‐to‐Bone Healing in a Rat Rotator Cuff Repair Model?

Yoon

Chung

Jung

et al. 2019

Journal Orthopaedic Research

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“…Recently, it was reported that the local single administration of TPTD (20 µg) with a collagen sponge is effective for repair of a rat calvarial defect (Auersvald et al, ). It was revealed that OCPcol with a local single administration of TPTD (1 or 0.1 µg) which is a low dosage similar to that for the treatment for osteoporosis, enhanced bone repair of the critical‐sized bone defect (Kajii et al, ), and its modality might be practical for improving effective bone regeneration safely.…”

Section: Introductionmentioning

confidence: 99%

Bone regeneration by freeze‐dried composite of octacalcium phosphate collagen and teriparatide

et al. 2018

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Objective Octacalcium phosphate (OCP) and collagen (col) composite (OCPcol) demonstrated superior bone regeneration properties, and its commercialization appears to be forthcoming. As a practical medical material for new combination products, we developed a freeze‐dried composite with OCPcol and teriparatide (TPTD) (OCPcolTPTDf), and investigated its bone regenerative properties. Materials and Methods A disk of OCPcol was made by mixing OCP granules and atelocollagen for medical use. Then, OCPcolTPTDf was prepared by impregnation of the OCPcol disk with 1.0 or 0.1 µg of TPTD solution (OCPcolTPTDf 1.0 and OCPcolTPTDf 0.1, respectively) followed by lyophilization. In vitro release profiles of TPTD from OCPcolTPTDf were determined using an enzyme‐linked immunosorbent assay. Implantation of OCPcolTPTDf or OCPcol was carried out for a rat critical‐sized calvarial defect. And five defects in each group were collected after 12 weeks of implantation. Results The retention–release profiles of TPTD from OCPcolTPTDf supported a higher degree of retention of TPTD. Radiographic, histological, and histomorphometric examinations indicated that regenerated bone was filled in most of the defects of the OCPcolTPTDf. Additionally, the OCPcolTPTDf groups showed significantly enhanced bone regeneration compared with the OCPcol group. Conclusions These results suggested that this newly developed bone regenerative composite could be a practical medical material.

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Effects of local single dose administration of parathormone on the early stages of osseointegration: A pre‐clinical study

et al. 2022

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The present study aimed to evaluate the effect of parathormone (PTH) administered directly to the implant's surface prior to insertion, using a large translational animal model. Sixty titanium implants were divided into four groups: (i) Collagen, control group, where implants were coated with Type‐I Bovine‐collagen, and three experimental groups, where implants received varying doses of PTH: (ii) 12.5, (iii) 25, and (iv) 50 μg, prior to placement. Fifteen female sheep (~2 years old, weighing ~65 kg) received four implants in an interpolated fashion in C3, C4 or C5 vertebral bodies. After 3‐, 6‐ and 12‐weeks, samples were harvested, histologically processed, qualitatively and quantitatively assessed for bone‐to‐implant contact (BIC) and bone area fraction occupancy (BAFO). BIC yielded lower values at 6‐weeks for 50 μg relative to the control group, with no significant differences, when compared to the 12.5‐ and 25‐μg. No significant differences were detected at 6‐weeks between collagen, 12.5‐ and 25‐μg groups. At 3‐ and 12‐weeks, no differences were detected for BIC among PTH groups. With respect to BAFO, no significant differences were observed between the control and experimental groups independent of PTH concentration and time in vivo. Qualitative observations at 3‐weeks indicated the presence of a more mature bone near the implant's surface with the application of PTH, however, no significant differences in new bone formation or healing patterns were observed at 6‐ and 12‐weeks. Single local application of different concentrations of PTH on titanium implant's surface did not influence the osseointegration at any time‐point evaluation in low‐density bone.

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The local administration of parathyroid hormone encourages the healing of bone defects in the rat calvaria: Micro-computed tomography, histological and histomorphometric evaluation

Cited by 18 publications

References 24 publications

Is a Local Administration of Parathyroid Hormone Effective to Tendon‐to‐Bone Healing in a Rat Rotator Cuff Repair Model?

Is a Local Administration of Parathyroid Hormone Effective to Tendon‐to‐Bone Healing in a Rat Rotator Cuff Repair Model?

Bone regeneration by freeze‐dried composite of octacalcium phosphate collagen and teriparatide

Effects of local single dose administration of parathormone on the early stages of osseointegration: A pre‐clinical study

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