The lncRNA SNHG15/miR‐18a‐5p axis promotes cell proliferation in ovarian cancer through activating Akt/mTOR signaling pathway

Zhang, Jingru; Jiang, Jing; Qiao, Zhiwei

doi:10.1002/jcb.29474

Cited by 13 publications

(11 citation statements)

References 33 publications

(70 reference statements)

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“…Subsequently, SNHG15 was investigated in tumor, and the SNHG15 expression was upregulated in cancers and high SNHG15 expression predicted poor prognosis, for instance, gastric cancer [ 17 ], lung cancer [ 18 ], colorectal cancer [ 29 ], and glioma [ 24 ]. Consistent with the recent evidence raised by Zhang et al [ 30 ], our results showed that SNHG15 was upregulated in ovarian cancer; it is noteworthy that our results were based on the GEO dataset and verified by the specimen of our own; this makes the results more reliable. In addition, the prognostic role of SNHG15 was identified by Kaplan-Meier plotter database due to our limited sample size and SNHG15 was correlated with poor PFS.…”

Section: Discussionsupporting

confidence: 92%

lncRNA SNHG15 Promotes Ovarian Cancer Progression through Regulated CDK6 via Sponging miR-370-3p

Wang

Ding

Yuan

et al. 2021

BioMed Research International

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Ovarian cancer is a kind of cancer from the female genital tract; the molecular mechanism still needs to be explored. lncRNA plays a vital role in tumorigenesis and development. Our aim was to identify oncogenic lncRNAs in ovarian cancer and explore the potential molecular mechanism. SNHG15 was initially identified by using GEO datasets (GSE135886 and GSE119054) and validated by tumor tissues and the cell line, identifying that SNHG15 was upregulated in ovarian cancer. Besides, high SNHG15 indicated poor prognosis in ovarian cancer. Furthermore, knockdown SNHG15 suppresses ovarian cancer proliferation and promotes apoptosis. Mechanistically, SNHG15 promotes proliferation through upregulated CDK6 via sponging miR-370-3p. Taken together, our findings emphasize the important role of SNHG15 in ovarian cancer, suggesting that SNHG15 may be a promising target for ovarian cancer.

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Section: Discussionsupporting

confidence: 92%

lncRNA SNHG15 Promotes Ovarian Cancer Progression through Regulated CDK6 via Sponging miR-370-3p

Wang

Ding

Yuan

et al. 2021

BioMed Research International

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“…Additionally, OC patients with low miR-18a-5p expression presented short survival. Consistently, miR-18a-5p is reported to be downregulated in OC cells and tissues [ 36 ]. miR-18a exerts repressive roles in OC cells, whose low expression is linked with the poor prognosis and malignant phenotype of OC patients [ 50 ].…”

Section: Discussionmentioning

confidence: 66%

“…miR-18a-5p is abundant in embryonic stem cell-derived small EVs [ 53 ]. miR-18a mimic could relieve OC cell migration and invasion [ 36 ]. Taken together, hMSC-EVs inhibited OC cell proliferation, migration, and invasion by carrying miR-18a-5p.…”

Section: Discussionmentioning

confidence: 99%

“…OC CAOV3/ES2 cells and normal ovarian epithelial cells IOSE80 [ 36 ] (Cell Bank of Chinese Academy of Sciences, Shanghai, China) were cultured in a RPMI-1640 cell culture medium (C11875500BT, Gibco, Grand Island, NY, USA) including 10% fetal bovine serum (FBS) (Gibco, C11108862) and 1% penicillin/streptomycin (Thermo Fisher, Waltham, MA, USA, 10378016). hMSCs (PromoCell, Heidelberg, Germany) were cultured in a Mesenchymal Stem Cell Growth Medium (Promocell).…”

Section: Methodsmentioning

confidence: 99%

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miR-18a-5p derived from mesenchymal stem cells-extracellular vesicles inhibits ovarian cancer cell proliferation, migration, invasion, and chemotherapy resistance

2022

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Objective Ovarian cancer (OC) is a major threat to women’s health. Mesenchymal stem cells (MSCs) are key regulators in cellular communication by secreting extracellular vesicles (EVs) that are involved in OC. This study probed into the mechanism of human MSCs derived-EVs (hMSC-EVs) in regulating OC cell growth and chemotherapy resistance. Methods hMSCs and EVs were isolated and identified. After adding EVs, the uptake of EVs by OC CAOV3/ES2 cells (for in vitro studies), and cell proliferation, migration, and invasion were detected. Downregulated miRNAs in hMSC-EVs were screened and miR-18a-5p expression in OC patients was detected. The prognosis of OC patients was analyzed. Binding sites of miR-18a-5p and NACC1 were predicted and validated. NACC1 expression in OC tissues was measured by RT-qPCR, and its correlation with miR-18a-5p was analyzed by Pearson method. AKT/mTOR pathway activation was assessed by WB. The cisplatin sensitivity of EVs-treated CAOV3 cells was evaluated via MTT assay and tested by tumor formation assay in nude mice. Results hMSC-EVs suppressed OC cell proliferation, migration, and invasion. miR-18a-5p was downregulated in OC and miR-18a-5p low expression was associated with a poor prognosis. EV-encapsulated miR-18a-5p targeted NACC1. NACC1 was upregulated in OC tissues. miR-18a-5p knockdown and NACC1 overexpression both annulled the inhibition of hMSC-EVs on OC cell growth. AKT and mTOR were elevated in OC and NACC1 activated the AKT/mTOR pathway in OC cells. hMSC-EVs promoted cisplatin sensitivity of OC cells by carrying miR-18a-5p. Conclusion hMSC-EVs-derived miR-18a-5p inhibits OC cell proliferation, migration, invasion, and chemotherapy resistance.

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“…SNHG15 is an lncRNA which plays critical roles in the development and progression of various cancers through regulation of Akt/mTOR signaling [153,154]. It has been reported as a prognostic marker in colorectal and liver cancers [155,156].…”

Section: Snhg15mentioning

confidence: 99%

Non-Coding RNAs in Pancreatic Cancer Diagnostics and Therapy: Focus on lncRNAs, circRNAs, and piRNAs

Hallak

Philip

et al. 2021

Cancers

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Pancreatic cancer is an aggressive malignance with high mortality. The lack of early diagnosis and effective therapy contributes to the high mortality of this deadly disease. For a long time being, the alterations in coding RNAs have been considered as major targets for diagnosis and treatment of pancreatic cancer. However, with the advances in high-throughput next generation of sequencing more alterations in non-coding RNAs (ncRNAs) have been discovered in different cancers. Further mechanistic studies have demonstrated that ncRNAs such as long noncoding RNAs (lncRNA), circular RNAs (circRNA) and piwi-interacting RNA (piRNA) play vital roles in the regulation of tumorigenesis, tumor progression and prognosis. In recent years, increasing studies have focused on the roles of ncRNAs in the development and progression of pancreatic cancer. Novel findings have demonstrated that lncRNA, circRNA, and piRNA are critically involved in the regulation of gene expression and cellular signal transduction in pancreatic cancer. In this review, we summarize the current knowledge of roles of lncRNA, circRNA, and piRNA in the diagnosis and prognosis of pancreatic cancer, and molecular mechanisms underlying the regulation of these ncRNAs and related signaling in pancreatic cancer therapy. The information provided here will help to find new strategies for better treatment of pancreatic cancer.

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The lncRNA SNHG15/miR‐18a‐5p axis promotes cell proliferation in ovarian cancer through activating Akt/mTOR signaling pathway

Cited by 13 publications

References 33 publications

lncRNA SNHG15 Promotes Ovarian Cancer Progression through Regulated CDK6 via Sponging miR-370-3p

lncRNA SNHG15 Promotes Ovarian Cancer Progression through Regulated CDK6 via Sponging miR-370-3p

miR-18a-5p derived from mesenchymal stem cells-extracellular vesicles inhibits ovarian cancer cell proliferation, migration, invasion, and chemotherapy resistance

Non-Coding RNAs in Pancreatic Cancer Diagnostics and Therapy: Focus on lncRNAs, circRNAs, and piRNAs

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