The lncRNA H19 interacts with miR-140 to modulate glioma growth by targeting iASPP

Zhao, Hong; Peng, Renjun; Liu, Qing; Liu, Dingyang; Du, Peng; Yuan, Jian; Peng, Gang; Liao, Yixiang

doi:10.1016/j.abb.2016.09.014

Cited by 75 publications

(63 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Jia P et al found that knockdown of H19 suppressed glioma induced angiogenesis by inhibiting miR-29a [43]. Similarly, Zhao H et al found that H19 might regulate the tumor growth and metastasis via miR-140 dependent iASPP regulation [44]. Our group previously stated the oncogenic role of H19 on promoting glioma cell invasion by inducing miR-675 expression [22].…”

Section: Discussionmentioning

confidence: 99%

H19 Functions as a Competing Endogenous RNA to Regulate EMT by Sponging miR-130a-3p in Glioma

Yin

Zeng

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: Glioma is one of the most devasting tumors and confers dismal prognosis. Long noncoding RNAs(lncRNAs) have emerged as important regulators in various tumors including glioma. A classic lncRNA-H19, which is found to be highly expressed in human glioma tissues and cell lines, and is associated with tumor progression thus predicating clinical outcomes in glioma patients. However, the overall biological functions and their mechanism of H19 in glioma are not fully understood. Methods: Firstly, we analyzed H19 alterations in different grades of glioma tissues through an analysis of 5 sequencing datasets and qRT-PCR was performed to confirm the results. Next, we evaluated the effect of H19 on glioma cells migration, invasion and EMT process. Luciferase assays and RIP assays were employed to figure out the correlation of H19 and SOX4. Results: H19 was overexpressed in glioma tissues. Down-regulation of H19 led to the inhibition of migration, invasion and EMT process with a reduction in N-cadherin and Vimentin. H19 and SOX4 are both direct target of miR-130a-3p. H19 could compete with SOX4 via sponging miR-130a-3p. Conclusion: Taken together, these results provide a possible function of H19 as an oncogene in glioma tissues and provide a potential new therapeutic strategy for human glioma.

show abstract

Section: Discussionmentioning

confidence: 99%

H19 Functions as a Competing Endogenous RNA to Regulate EMT by Sponging miR-130a-3p in Glioma

Yin

Zeng

et al. 2018

Cell Physiol Biochem

View full text Add to dashboard Cite

show abstract

“…Of these five groups, antisense lncRNA, a type of endogenous lncRNA complementary to the corresponding transcription sequence, comprises 50–70% of all lncRNA . The oncogenic role of lncRNAs has been identified in various human cancers . Numerous antisense lncRNAs have been identified as crucial regulators in cancerous cellular processes dependent on relative endogenous genes .…”

Section: Introductionmentioning

confidence: 99%

“…16 The oncogenic role of lncRNAs has been identified in various human cancers. 17,18 Numerous antisense lncRNAs have been identified as crucial regulators in cancerous cellular processes dependent on relative endogenous genes. [19][20][21] However, antisense lncRNAs modulating CC progression in this way require further investigation.…”

Section: Introductionmentioning

confidence: 99%

ELK1‐activated GPC3‐AS1/GPC3 axis promotes the proliferation and migration of cervical cancer cells

Zhu

2019

The Journal of Gene Medicine

View full text Add to dashboard Cite

Background Long non‐coding RNA (lncRNA) is reported to be involved in multiple biological processes in numerous human tumors. Furthermore, an increasing number of studies have confirmed the involvement of lncRNA in the initiation and development of human cancers, including cervical cancer (CC). Methods The present study explored the potential role of lncRNA glypican 3 antisense transcript 1 (GPC3‐AS1) with respect to regulating CC cell proliferation and migration. Results A quantitative reverse transcriptase‐polymerase chain reaction confirmed that GPC3‐AS1 was up‐regulated in CC cells compared to normal CRL‐2614 cells. Loss‐of‐function assays demonstrated the negative effect of GPC3‐AS1 depletion on CC cell proliferation and migration. GPC3‐AS1 positively regulated its nearby gene glypican 3 (GPC3). Significant up‐regulation of GPC3 was also observed in CC cells, consistently with GPC3‐AS1. In addition, GPC3‐AS1 and GPC3 synergistically promoted the proliferative and migratory abilities of CC cells. Mechanistic investigation showed that ELK1 acted as the transcription activator of GPC3‐AS1 and GPC3, thus contributing to their up‐regulation in CC cells. Rescue assays indicated that the ELK1‐induced GPC3‐AS1/GPC3 axis promoted cell proliferation and migration in CC. Conclusions The results of the present study have revealed a novel molecular pathway that can regulate CC cell proliferation and migration, thus providing a new basis for investigating the molecular mechanism associated with CC progression.

show abstract

“…IASPP is an evolutionarily conserved inhibitor of p53; inhibition of iASPP by RNAmediated interference or antisense RNA in human cells, induces p53dependent apoptosis [Bergamaschi et al, 2003]. According to previous studies, targeting iASPP to inhibit its expression so that to suppress tumor cell proliferation and induce tumor cell apoptosis present a promising strategy against tumors Cai et al, 2012;Zhao et al, 2016]. In the present study, overexpressing miR-184 could downregulate iASPP protein expression, while miR-184 knockdown could promote iASPP protein expression.…”

Section: Discussionmentioning

confidence: 51%

MicroRNA-184 Modulates Human Central Nervous System Lymphoma Cells Growth and Invasion by Targeting iASPP

Liang

Wei

et al. 2017

J. Cell. Biochem.

View full text Add to dashboard Cite

Central nervous system lymphoma (CNSL) remains a diagnostical and therapeutical challenge. MiRNAs post-transcriptionally regulate expression of targeted mRNAs through binding to their 3' UTR to inhibit their translation or promote their degradation. Oncoprotein inhibitory member of the ASPP family (iASPP), a key inhibitor of tumor suppressor p53, has been reported to play oncogenic role in cancers. Our present study was aimed to determine whether the miR-184/iASPP axis is involved in the proliferation and invasion of CNSL. A reduced level of miR-184 was observed in CNSL tissues. Exogenous miR-184 inhibited cell survival and invasion, as well as the tumor volumes, while miR-184 inhibition could reverse this process. The RNA and protein levels of iASPP were significantly inhibited by miR-184, and the 3' UTR of iASPP was shown to be a target of miR-184. The expression of iASPP was up-regulated in CNSL tissues, compared to that of the normal brain tissues. The inhibition of iASPP by shRNA iASPP significantly repressed CNSL cells' proliferation and invasion, and reduced the volume of the tumor. Besides, iASPP overexpression could partly restore the suppressive effect of miR-184 on CNSL cell proliferation and invasive capability. We also revealed that miR-184/iASPP axis regulated the proliferation and invasion via PI3K/Akt signaling pathway, which presents a novel potential therapy for intervention of CNSL. Taken together, our findings revealed the detailed role of the miR-184/iASPP axis in CNSL and this axis might modulate the proliferation and invasion of CNSL via regulating the PI3K/Akt signaling pathway. J. Cell. Biochem. 118: 2645-2653, 2017. © 2017 Wiley Periodicals, Inc.

show abstract

The lncRNA H19 interacts with miR-140 to modulate glioma growth by targeting iASPP

Cited by 75 publications

References 31 publications

H19 Functions as a Competing Endogenous RNA to Regulate EMT by Sponging miR-130a-3p in Glioma

H19 Functions as a Competing Endogenous RNA to Regulate EMT by Sponging miR-130a-3p in Glioma

ELK1‐activated GPC3‐AS1/GPC3 axis promotes the proliferation and migration of cervical cancer cells

MicroRNA-184 Modulates Human Central Nervous System Lymphoma Cells Growth and Invasion by Targeting iASPP

Contact Info

Product

Resources

About