The lncRNA Gm15622 stimulates SREBP-1c expression and hepatic lipid accumulation by sponging the miR-742-3p in mice

Ma, Minjuan; Duan, Rui; Shen, Lulu; Liu, Mengting; Ji, Yaya; Zhou, Hao; Li, Changxian; Liang, Tingming; Li, Xiangcheng; Guo, Li

doi:10.1194/jlr.ra120000664

Cited by 40 publications

(23 citation statements)

References 41 publications

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“…Studies indicated that miR-495 participated in the regulation of inflammatory response, which acted as a negative modulator of inflammation-motivating pathways [ 55 , 56 ]. miR-742-3p was only reported to be involved in excessive lipid deposition [ 57 ], and miR-126b was essential for lymphatic system development in mammals [ 58 ]. In addition, no article reported the relationship between miR-672-3p and diseases.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis Exploring the Molecular Mechanisms of Hanchuan Zupa Granules in Alleviating Asthma in Rat

Yin

Fan

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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Context. Hanchuan Zupa granule (HCZP), as a Chinese traditional medicine, is used to treat asthma. Objective. To investigate the molecular mechanisms of HCZP treatment of asthma. Materials and Methods. Thirty Sprague Dawley (SD) rats were divided into normal, asthma, and HCZP groups (n = 10). The asthma model was sensitized by 1 mg ovalbumin (OVA)/aluminum hydroxide Al(OH)3mixture and then challenged with 1% aerosolized OVA for four weeks. Rats in the HCZP group received 10.08 g/kg/d HCZP for four weeks during OVA challenge. Then, lung tissues of rats in each group were collected for RNA sequencing. Moreover, the expression level of some core genes was detected by using western blotting and immunohistochemistry. Results. Inflammatory cell infiltration and pathological damage of the lungs improved in the HCZP group. Compared with the asthma group (0.049 ± 0.002 mm2/mm; 0.036 ± 0.006 mm2/mm; and 0.014 ± 0.001 mm2/mm), total wall thickness (0.042 ± 0.001 mm2/mm), inner wall thickness (0.013 ± 0.001 mm2/mm), and smooth muscle layer thickness (0.012 ± 0.001 mm2/mm) significantly decreased in the HCZP group. Bioinformatics analysis showed that hub genes such as bradykinin receptor B2 (Bdkrb2) and CD4 molecule (Cd4) had different expression patterns between model and HCZP groups. Two transcription factors, forkhead box Q1 (Foxq1) and nuclear factor of activated T cells 2 (Nfatc2), served important regulatory roles in asthma. Compared with the model group, Bdkrb2 protein expression increased and Nfatc2 protein expression decreased in the HCZP group. Discussion and Conclusion. HCZP could alleviate asthma via regulating the expression of several hub genes, which might serve as therapeutic targets for asthma. However, the mechanism of these genes will be studied in the future.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis Exploring the Molecular Mechanisms of Hanchuan Zupa Granules in Alleviating Asthma in Rat

Yin

Fan

et al. 2021

Evidence-Based Complementary and Alternative Medicine

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“…However, most of these studies focused on screening differentially expressed genes (DEGs) using RNA-seq without sufficient exploration of the function and mechanisms of the DEGs. In previous papers, a number of lncRNAs have been well characterized to be involved in adipose tissue accumulation in cells or rodents [50,64]. These studies could provide insights into the important role and application of lncRNAs in regulating the development of adipose tissue in farm animals.…”

Section: A C C E P T E D a R T I C L Ementioning

confidence: 96%

The role of long noncoding RNAs in livestock adipose tissue deposition

et al. 2021

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With the development of sequencing technology, numerous , long noncoding RNAs (lncRNAs) have been discovered and annotated. Increasing evidence has shown that lncRNAs play an essential role in regulating many biological and pathological processes, especially in cancer. However, there have been few studies on the roles of lncRNAs in livestock production. In animal products, meat quality and lean percentage are vital economic traits closely related to adipose tissue deposition. However, adipose tissue accumulation is also a pivotal contributor to obesity, diabetes, atherosclerosis, and many other diseases, as demonstrated by human studies. In livestock production, the mechanism by which lncRNAs regulate adipose tissue deposition is still unclear. In addition, the phenomenon that different animal species have different adipose tissue accumulation abilities is not well understood. In this review, we summarize the characteristics of lncRNAs and their four functional archetypes and review the current knowledge about lncRNA functions in adipose tissue deposition in livestock species. This review could provide theoretical significance to explore the functional mechanisms of lncRNAs in adipose tissue accumulation in animals.

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“…Indeed, the lncRNA Blnc1, implicated in adipogenesis and obesity, was found upregulated in obese and NAFLD mice, activating SREBP1c and hepatic lipogenesis, thus aggravating disease progression 249 . Gm15622 was also found upregulated in the liver of obese mice fed a HFD, exerting its mechanism of action sponging miR‐742‐3p, subsequently upregulating SREBP1c 250 . Moreover, its inactivation abrogates HFD‐induced hepatic steatosis, suggesting also in this case a therapeutic window 249 .…”

Section: Lncrnas In Obesity‐associated Diseasesmentioning

confidence: 98%

Role of long non‐coding RNAs in adipogenesis: State of the art and implications in obesity and obesity‐associated diseases

et al. 2021

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Summary Obesity is an evolutionary, chronic, and relapsing disease that consists of a pathological accumulation of adipose tissue able to increase morbidity for high blood pressure, type 2 diabetes, metabolic syndrome, and obstructive sleep apnea in adults, children, and adolescents. Despite intense research over the last 20 years, obesity remains today a disease with a complex and multifactorial etiology. Recently, long non‐coding RNAs (lncRNAs) are emerging as interesting new regulators as different lncRNAs have been found to play a role in early and late phases of adipogenesis and to be implicated in obesity‐associated complications onset. In this review, we discuss the most recent advances on the role of lncRNAs in adipocyte biology and in obesity‐associated complications. Indeed, more and more researchers are focusing on investigating the underlying roles that these molecular modulators could play. Even if a significant number of evidence is correlation‐based, with lncRNAs being differentially expressed in a specific disease, recent works are now focused on deeply analyzing how lncRNAs can effectively modulate the disease pathogenesis onset and progression. LncRNAs possibly represent new molecular markers useful in the future for both the early diagnosis and a prompt clinical management of patients with obesity.

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The lncRNA Gm15622 stimulates SREBP-1c expression and hepatic lipid accumulation by sponging the miR-742-3p in mice

Cited by 40 publications

References 41 publications

Transcriptomic Analysis Exploring the Molecular Mechanisms of Hanchuan Zupa Granules in Alleviating Asthma in Rat

Transcriptomic Analysis Exploring the Molecular Mechanisms of Hanchuan Zupa Granules in Alleviating Asthma in Rat

The role of long noncoding RNAs in livestock adipose tissue deposition

Role of long non‐coding RNAs in adipogenesis: State of the art and implications in obesity and obesity‐associated diseases

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