The liquid condensed diffusional transition of dipalmitoylphosphoglycerocholine in monolayers

Denicourt, Normand; Tancrède, Pierre; Brullemans, Marc; Teissié, Justin

doi:10.1016/0301-4622(89)80008-0

Cited by 10 publications

(8 citation statements)

References 35 publications

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“…The present results are consistent with previous simulation results for Langmuir monolayers, where one found that the single-particle diffusion coefficient decreases for decreasing area per lipid (increasing surface pressure). Similar trends have also been observed in experiments. , …”

Section: Resultssupporting

confidence: 89%

“…Similar trends have also been observed in experiments. 33,34 Another interesting observation regards diffusion in the pure PL system. Diffusion of phospholipids in the pure phospholipid system is very high compared to the corresponding tear fluid systems, and for pure PL the diffusion even increases as the pressure is increased, illustrating the unstable nature of the pure PL layer under high surface pressures.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Role of Neutral Lipids in Tear Fluid Lipid Layer: Coarse-Grained Simulation Study

et al. 2012

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Tear fluid lipid layer (TFLL) residing at the air-water interface of tears has been recognized to play an important role in the development of dry eye syndrome. Yet, the composition, structure, and mechanical properties of TFLL are only partly known. Here, we report results of coarse-grained simulations of a lipid layer comprising phospholipids, free fatty acids, cholesteryl esters, and triglycerides at the air-water interface to shed light on the properties of TFLL. We consider structural as well as dynamical properties of the lipid layer as a function of surface pressure. Simulations revealed that neutral lipids reside heterogeneously between phospholipids at relatively low pressures but form a separate hydrophobic phase with increasing surface pressure, transforming the initial lipid monolayer to a two-layered structure. When the model of TFLL was compared to a one-component phospholipid monolayer system, we found drastic differences in both structural and dynamical properties that explain the prominent role of neutral lipids as stabilizers of the TFLL. Based on our results, we suggest that neutral lipids are able to increase the stability of the TFLL by modulating its dynamical and structural behavior, which is important for the proper function of tear film.

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Section: Resultssupporting

confidence: 89%

Section: ■ Results and Discussionmentioning

confidence: 99%

Role of Neutral Lipids in Tear Fluid Lipid Layer: Coarse-Grained Simulation Study

et al. 2012

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“…It is of interest that the increase in I e /I m ratio for PPDPC levels off at approximately X LBPA = 0.05, similarly to the effect of LBPA 18:1 on DPH anisotropy. Based on the above, it seems plausible that the hydration of the membrane interphase is increased at least up to X LBPA = 0.05, thus explaining the increased lateral diffusion (Denicourt et al, 1989;Lehtonen and Kinnunen, 1994). Our data further suggest that exceeding X LBPA = 0.05 does not have any major impact on the dynamics of POPC bilayers in the pH range 3.0-7.4.…”

Section: Discussionmentioning

confidence: 59%

Intermolecular interactions of lysobisphosphatidic acid with phosphatidylcholine in mixed bilayers

Holopainen

Söderlund

Alakoskela

et al. 2005

Chemistry and Physics of Lipids

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“…On one hand, the flip-flop and lateral diffusion of DOPC molecules in the fluidic phase is very rapid. It is reasonable to consider that the heavy TR-DHPE molecules were pushed or motivated synchronously by the more mobile DOPC molecules. , It should be emphasized that DOPC was considered to be kept in the fluidic phase at room temperature for all samples. Since the TR-DHPE molecules were assumed synchronous to DOPC, the TR-DHPE distribution rapidly achieved a homogeneous distribution in the DOPC fluidic phase in both the LB and LS leaflets.…”

Section: Resultsmentioning

confidence: 99%

“…Carafoli has reported intracellular calcium homeostasis, which influences one of the important functions of cell membranes: singaling . Denicourt and co-workers have demonstrated the phase transition of a single phospholipid monolayer on air and sodium chloride interfaces and measured the influence of ionic strength . Transferring the monolayer of binary mixture from air/aqueous interfaces to mica, Hollars and Dunn have characterized the convolution of phase structure in solid-supported lipid bilayers (SLBs) by using atomic force microscopy (AFM) .…”

Section: Introductionmentioning

confidence: 99%

Lipid Reassembly in Asymmetric Langmuir–Blodgett/Langmuir–Schaeffer Bilayers

et al. 2012

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Molecular-reorganization-induced morphology alteration in asymmetric substrate-supported lipid bilayers (SLBs) was directly visualized by means of atomic force microscopy (AFM) and total internal reflection fluorescence (TIRF) microscopy. SLB samples were fabricated on mica-on-glass and glass substrates by Langmuir-Blodgett (LB)/Langmuir-Schaeffer (LS) using binary lipid mixtures, namely, 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)/1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and ternary mixtures DOPC/DPPC/1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS), labeled with 0.2 mol % Texas Red 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine triethylammonium salt (TR-DHPE) dye. Phase segregations were characterized by TIRF imaging, and DPPC-enriched domain structures were also observed. Interestingly for ∼40% (n = 6) of the samples with binary mixtures in the LB leaflet and a single component in the LS leaflet, that is, (DOPC/DPPC)(LB)+DOPC(LS), the contrast of the DPPC domains changed from the original dark (without dye) to bright (more TR dye partitioning) on TIRF images, returning to dark again. This contrast reverse was also correlated to AFM height images, where a DPPC-DPPC gel phase was spotted after the TIRF image contrast returned to dark. The rupture force mapping results measured on these binary mixture samples also confirmed unambiguously the formation of DPPC-DPPC gel domain components during the contrast change. The samples were tracked over 48 h to investigate the lipid molecule movements in both the DPPC domains and the DOPC fluid phase. The fluorescence contrast changes from bright to dark in SLBs indicate that the movement of dye molecules was independent of the movement of lipid molecules. In addition, correlated multimodal imaging using AFM, force mapping, and fluorescence provides a novel route to uncover the reorganization of lipid molecules at the solid-liquid interface, suggesting that the dynamics of dye molecules is highly structure dependent.

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The liquid condensed diffusional transition of dipalmitoylphosphoglycerocholine in monolayers

Cited by 10 publications

References 35 publications

Role of Neutral Lipids in Tear Fluid Lipid Layer: Coarse-Grained Simulation Study

Role of Neutral Lipids in Tear Fluid Lipid Layer: Coarse-Grained Simulation Study

Intermolecular interactions of lysobisphosphatidic acid with phosphatidylcholine in mixed bilayers

Lipid Reassembly in Asymmetric Langmuir–Blodgett/Langmuir–Schaeffer Bilayers

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