THE LIQUID CHROMATOGRAPHIC ANALYSIS OF VITAMIN K₁IN SOY BASED INFANT FORMULA USING MATRIX SOLID PHASE DISPERSION

Abstract

A liquid chromatographic method is described for vitamin K 1 in soy based infant formula. The vitamins are extracted from infant formula by matrix solid phase dispersion (MSPD) and quantitated by reversed phase chromatography with fluorescence detection. Vitamin K 1 is converted to the fluorescent hydroquinone with a post column zinc reductive reactor. The limit of detection is 12 pg and the limit of quantitation is 38 pg on-column. Linear response ranged from 0.70 -11.0 ng/mL (r 2 = 0.998). Recoveries were determined on an analyte-fortified blank material for soy based infant formula and averaged 92.5% (n = 25) for vitamin K 1 . 423

Liquid Chromatographic Analysis of Vitamin K1in Medical Foods Using Matrix Solid-Phase Dispersion

“…The retention time for vitamin K was observed to shift considerably from the earlier work (Chase et al, 1999a, b) from 4 to 8.5 min; however, the total run time only increased by 1 min. The addition of hexane to the mobile phase, standards and samples as previously discussed (Chase et al, 1999a) is essential in order to maintain low column back pressure and good recoveries. There were no observed di$culties with the miscibility of hexane in methanol at levels of 10% (v/v).…”

“…A ZRM originally used for other fat-soluble vitamin method development studies was utilized for vitamin K (Chase et al, 1998). Five pounds of a powdered medical food were obtained from a domestic manufacturer that was formulated without the addition of fat-and water-soluble vitamins.…”

Phytonadione Content in Branded Intravenous Fat Emulsions

“…HPLC has conventionally been the preferred way of analyzing vitamin K in foods and other biological matrices because of its highly sensitive detection and quantification. However, complex pretreatment is required to eliminate interfering components from the sample and/or to improve analytical sensitivity 5 . ‐ 9 In addition, HPLC analysis requires analytical time throughputs of up to 30 minutes per sample, which could lead to vitamin loss.…”

“…High‐performance liquid chromatography (HPLC) is the preferential method of analyzing vitamin K1 content in foods and other biological matrices. However, HPLC can be inaccurate in the presence of complex matrices, while liquid chromatography–mass spectrometry (LC‐MS) with pretreatment may be promising 4 . ‐ 9 Therefore, in this study, we evaluate the phytonadione content of different branded IVFE by an LC‐MS method that we believe is more appropriate to detect FSV trace levels in a lipid matrix.…”

Supplémentation en vitamine K lors de la nutrition parentérale chez l’enfant

“…Différentes méthodes chromatographiques sont décrites dans la littérature et utilisent la spectrométrie de masse [6] ou la fluorescence après réduction en post-colonne [7][8][9][10] comme modes de détection. Cette technique n'étant pas utilisable dans notre milieu hospitalier, une technique de dosage a été mise au point et validée.…”