The Lipid Transfer Protein StarD7: Structure, Function,  and Regulation

Flores-Martín, Jésica; Rena, Viviana; Angeletti, Sofía; Panzetta-Dutari, Graciela M.; Genti-Raimondi, Susana

doi:10.3390/ijms14036170

Cited by 27 publications

(25 citation statements)

References 92 publications

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“…S14). STARD7 has been previously implicated in choriocarcinoma, colorectal cancer, breast, and lung cancers (24). This strong interaction between the amplified regions on chromosomes 2 and 8 had not been recognized before.…”

Section: Structure and Function Of The Hsrmentioning

confidence: 78%

Nucleome Analysis Reveals Structure–Function Relationships for Colon Cancer

Seaman

Chen

Brown

et al. 2017

Molecular Cancer Research

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Chromosomal translocations and aneuploidy are hallmarks of cancer genomes; however, the impact of these aberrations on the nucleome (i.e., nuclear structure and gene expression) is not yet understood. Here, the nucleome of the colorectal cancer cell line HT-29 was analyzed using chromosome conformation capture (Hi-C) to study genome structure, complemented by RNA sequencing (RNA-seq) to determine the consequent changes in genome function. Importantly, translocations and copy number changes were identified at high resolution from Hi-C data and the structure-function relationships present in normal cells were maintained in cancer. In addition, a new copy number-based normalization method for Hi-C data was developed to analyze the effect of chromosomal aberrations on local chromatin structure. The data demonstrate that at the site of translocations, the correlation between chromatin organization and gene expression increases; thus, chromatin accessibility more directly reflects transcription. In addition, the homogeneously staining region of chromosome band 8q24 of HT-29, which includes the MYC oncogene, interacts with various loci throughout the genome and is composed of open chromatin. The methods, described herein, can be applied to the assessment of the nucleome in other cell types with chromosomal aberrations. Findings show that chromosome conformation capture identifies chromosomal abnormalities at high resolution in cancer cells and that these abnormalities alter the relationship between structure and function. .

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Section: Structure and Function Of The Hsrmentioning

confidence: 78%

Nucleome Analysis Reveals Structure–Function Relationships for Colon Cancer

Seaman

Chen

Brown

et al. 2017

Molecular Cancer Research

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“…StarD7 has been shown to interact with membranes (42–44), presumably as a result of its role in transferring phospholipid to membrane structures. To determine whether PFA0210c shares similar characteristics, we next sought to determine whether PFA0210c associates with membranes in the parasitized erythrocyte.…”

Section: Resultsmentioning

confidence: 99%

Identification of a Plasmodium falciparum Phospholipid Transfer Protein

Ooij¹,

Withers‐Martinez²,

Ringel³

et al. 2013

Journal of Biological Chemistry

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Background: PFA0210c is an exported Plasmodium falciparum protein.Results: PFA0210c is a phospholipid transfer protein with similarities to proteins of the family of START domain-containing proteins and can transfer various phospholipids between membranes in vitro.Conclusion: PFA0210c and its orthologs are phospholipid transfer proteins with a broad specificity.Significance: Identification of the function of conserved Plasmodium proteins allows deeper insight into the basis of pathogenesis.

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“…The best characterized human START proteins are STARD2, STARD7, and STARD10, which all transfer phosphatidylcholine (and additionally phosphatidylethanolamine in the case of STARD10) (11–13). Although their exact functions are unclear, the proteins appear to have a role in the transfer of phospholipids from the endoplasmic reticulum to mitochondria and possibly the plasma membrane (14, 15). What is also not understood is whether and how the transfer of phospholipids by these proteins is regulated.…”

Section: Introductionmentioning

confidence: 99%

Regulation and Essentiality of the StAR-related Lipid Transfer (START) Domain-containing Phospholipid Transfer Protein PFA0210c in Malaria Parasites

Hill

Ringel

Knuepfer

et al. 2016

Journal of Biological Chemistry

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StAR-related lipid transfer (START) domains are phospholipid- or sterol-binding modules that are present in many proteins. START domain-containing proteins (START proteins) play important functions in eukaryotic cells, including the redistribution of phospholipids to subcellular compartments and delivering sterols to the mitochondrion for steroid synthesis. How the activity of the START domain is regulated remains unknown for most of these proteins. The Plasmodium falciparum START protein PFA0210c (PF3D7_0104200) is a broad-spectrum phospholipid transfer protein that is conserved in all sequenced Plasmodium species and is most closely related to the mammalian START proteins STARD2 and STARD7. PFA0210c is unusual in that it contains a signal sequence and a PEXEL export motif that together mediate transfer of the protein from the parasite to the host erythrocyte. The protein also contains a C-terminal extension, which is very uncommon among mammalian START proteins. Whereas the biochemical properties of PFA0210c have been characterized, the function of the protein remains unknown. Here, we provide evidence that the unusual C-terminal extension negatively regulates phospholipid transfer activity. Furthermore, we use the genetically tractable Plasmodium knowlesi model and recently developed genetic technology in P. falciparum to show that the protein is essential for growth of the parasite during the clinically relevant asexual blood stage life cycle. Finally, we show that the regulation of phospholipid transfer by PFA0210c is required in vivo, and we identify a potential second regulatory domain. These findings provide insight into a novel mechanism of regulation of phospholipid transfer in vivo and may have important implications for the interaction of the malaria parasite with its host cell.

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The Lipid Transfer Protein StarD7: Structure, Function, and Regulation

Cited by 27 publications

References 92 publications

Nucleome Analysis Reveals Structure–Function Relationships for Colon Cancer

Nucleome Analysis Reveals Structure–Function Relationships for Colon Cancer

Identification of a Plasmodium falciparum Phospholipid Transfer Protein

Regulation and Essentiality of the StAR-related Lipid Transfer (START) Domain-containing Phospholipid Transfer Protein PFA0210c in Malaria Parasites

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